Study of Single-dose Toxicity of Guseonwangdo-go Glucose Intramuscular Injection in Sprague-Dawley Rats
Objectives: This study was performed to analyze single-dose intramuscular toxicity of Guseonwangdo-go glucose pharmacopuncture. Methods: Eighty six-week-old Sprague-Dawley rats were divided into two large groups of forty rats; Guseonwangdo-go glucose 5% and Guseonwangdo-go glucose 20% groups. Each...
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| Format: | Article |
| Language: | English |
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Korean Pharmacopuncture Institute
2014-03-01
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| Series: | Journal of Pharmacopuncture |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3831/KPI.2014.17.007 |
| _version_ | 1818147461366022144 |
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| author | Jo Su-jeong Kim Sung-chul Kim Yu-jong Kim Kap-sung Lee Seung-deok |
| author_facet | Jo Su-jeong Kim Sung-chul Kim Yu-jong Kim Kap-sung Lee Seung-deok |
| author_sort | Jo Su-jeong |
| collection | DOAJ |
| description | Objectives: This study was performed to analyze single-dose intramuscular toxicity of Guseonwangdo-go glucose pharmacopuncture.
Methods: Eighty six-week-old Sprague-Dawley rats were divided into two large groups of forty rats; Guseonwangdo-go glucose 5% and Guseonwangdo-go glucose 20% groups. Each group was sub-divided into four smaller groups of five males and five females, with the following dosages of pharmacopuncture being administered by intramuscular (IM) injection in each group: group 1 (G1, control group): 1.0 mL of normal saline solution, group 2 (G2, low-dose group): 0.1 mL, group 3 (G3, mid-dose group): 0.5 mL, and group 4 (G4, high-dose group): 1.0 mL.
Results: No mortalities or clinical signs were observed in any group. Also, no significant changes in body weights or in hematological/biochemical analyses were observed between the control and the experimental groups during necropsy or histopathology.
Conclusion: The above findings suggest that the lethal dose of Guseonwangdo-go glucose 5% and 20% pharmacopuncture administered via IM injection is more than 1.0 mL per animal in both male and female rats. Further studies on the repeated-dose toxicity of Guseonwangdo-go glucose should be conducted to yield more concrete data. |
| first_indexed | 2024-12-11T12:35:37Z |
| format | Article |
| id | doaj.art-1ae17caff3474309821927918a85aab1 |
| institution | Directory Open Access Journal |
| issn | 2093-6966 2234-6856 |
| language | English |
| last_indexed | 2024-12-11T12:35:37Z |
| publishDate | 2014-03-01 |
| publisher | Korean Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Pharmacopuncture |
| spelling | doaj.art-1ae17caff3474309821927918a85aab12022-12-22T01:07:08ZengKorean Pharmacopuncture InstituteJournal of Pharmacopuncture2093-69662234-68562014-03-01171515810.3831/KPI.2014.17.007Study of Single-dose Toxicity of Guseonwangdo-go Glucose Intramuscular Injection in Sprague-Dawley RatsJo Su-jeong 0Kim Sung-chul 1Kim Yu-jong 2Kim Kap-sung 3Lee Seung-deok 4Department of Acupuncture & Moxibustion Medicine, Dongguk University College of Oriental Medicine, Goyang, KoreaDepartment of Acupuncture & Moxibustion Medicine, Wonkwang University College of Oriental Medicine, Gwangju, KoreaDepartment of Acupuncture & Moxibustion Medicine, Dongguk University College of Oriental Medicine, Goyang, KoreaDepartment of Acupuncture & Moxibustion Medicine, Dongguk University College of Oriental Medicine, Goyang, KoreaDepartment of Acupuncture & Moxibustion Medicine, Dongguk University College of Oriental Medicine, Goyang, KoreaObjectives: This study was performed to analyze single-dose intramuscular toxicity of Guseonwangdo-go glucose pharmacopuncture. Methods: Eighty six-week-old Sprague-Dawley rats were divided into two large groups of forty rats; Guseonwangdo-go glucose 5% and Guseonwangdo-go glucose 20% groups. Each group was sub-divided into four smaller groups of five males and five females, with the following dosages of pharmacopuncture being administered by intramuscular (IM) injection in each group: group 1 (G1, control group): 1.0 mL of normal saline solution, group 2 (G2, low-dose group): 0.1 mL, group 3 (G3, mid-dose group): 0.5 mL, and group 4 (G4, high-dose group): 1.0 mL. Results: No mortalities or clinical signs were observed in any group. Also, no significant changes in body weights or in hematological/biochemical analyses were observed between the control and the experimental groups during necropsy or histopathology. Conclusion: The above findings suggest that the lethal dose of Guseonwangdo-go glucose 5% and 20% pharmacopuncture administered via IM injection is more than 1.0 mL per animal in both male and female rats. Further studies on the repeated-dose toxicity of Guseonwangdo-go glucose should be conducted to yield more concrete data.http://dx.doi.org/10.3831/KPI.2014.17.007Guseonwangdo-gointramuscular toxicitypharmacopuncturesingle-dose toxicity |
| spellingShingle | Jo Su-jeong Kim Sung-chul Kim Yu-jong Kim Kap-sung Lee Seung-deok Study of Single-dose Toxicity of Guseonwangdo-go Glucose Intramuscular Injection in Sprague-Dawley Rats Journal of Pharmacopuncture Guseonwangdo-go intramuscular toxicity pharmacopuncture single-dose toxicity |
| title | Study of Single-dose Toxicity of Guseonwangdo-go Glucose Intramuscular Injection in Sprague-Dawley Rats |
| title_full | Study of Single-dose Toxicity of Guseonwangdo-go Glucose Intramuscular Injection in Sprague-Dawley Rats |
| title_fullStr | Study of Single-dose Toxicity of Guseonwangdo-go Glucose Intramuscular Injection in Sprague-Dawley Rats |
| title_full_unstemmed | Study of Single-dose Toxicity of Guseonwangdo-go Glucose Intramuscular Injection in Sprague-Dawley Rats |
| title_short | Study of Single-dose Toxicity of Guseonwangdo-go Glucose Intramuscular Injection in Sprague-Dawley Rats |
| title_sort | study of single dose toxicity of guseonwangdo go glucose intramuscular injection in sprague dawley rats |
| topic | Guseonwangdo-go intramuscular toxicity pharmacopuncture single-dose toxicity |
| url | http://dx.doi.org/10.3831/KPI.2014.17.007 |
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