Isolation, immunochemical demonstration of field strains of porcine group A rotaviruses and electrophoretic analysis of RNA segments of group A and C rotaviruses
Rotaviruses are major cause of acute diarrhea in animals and humans which can result in huge economic losses in farm animals including pigs. We collected 195 samples of feces of diarrhoeic animals. Rotavirus was demonstrated by electron microscopy using the method of negative staining in 27 samples...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2006-05-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200605-0013_isolation-immunochemical-demonstration-of-field-strains-of-porcine-group-a-rotaviruses-and-electrophoretic-ana.php |
| _version_ | 1797897708619431936 |
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| author | R. Smitalova L. Rodak I. Psikal B. Smid |
| author_facet | R. Smitalova L. Rodak I. Psikal B. Smid |
| author_sort | R. Smitalova |
| collection | DOAJ |
| description | Rotaviruses are major cause of acute diarrhea in animals and humans which can result in huge economic losses in farm animals including pigs. We collected 195 samples of feces of diarrhoeic animals. Rotavirus was demonstrated by electron microscopy using the method of negative staining in 27 samples and by ELISA test using monoclonal antibodies to the group antigen VP6 in 44 samples. Nine samples were selected for virus isolation. Three virus isolates (P375/4, P410/4 and P646/1) were successfully adapted to growth in cell line MA-104. These isolates were allocated to group A rotaviruses based on ELISA, immunoperoxidase test and electropherotype analysis. Electropherotype analysis demonstrated changes during passage in cell line in two of the three isolates. The selected sample P543/1 proved negative in ELISA in a fecal sample. Electropherotype analysis of this sample revealed a "longer" electropherotype profile. The profile was suggestive of group C rotavirus. Rotavirus group C was confirmed by RT-PCR and by sequence analysis in this sample. |
| first_indexed | 2024-04-10T08:02:37Z |
| format | Article |
| id | doaj.art-1ae2e55e473748698cea99f5b39c986c |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:02:37Z |
| publishDate | 2006-05-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-1ae2e55e473748698cea99f5b39c986c2023-02-23T03:49:21ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922006-05-0151528829510.17221/5548-VETMEDvet-200605-0013Isolation, immunochemical demonstration of field strains of porcine group A rotaviruses and electrophoretic analysis of RNA segments of group A and C rotavirusesR. Smitalova0L. Rodak1I. Psikal2B. Smid3Veterinary Research Institute, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicVeterinary Research Institute, Brno, Czech RepublicRotaviruses are major cause of acute diarrhea in animals and humans which can result in huge economic losses in farm animals including pigs. We collected 195 samples of feces of diarrhoeic animals. Rotavirus was demonstrated by electron microscopy using the method of negative staining in 27 samples and by ELISA test using monoclonal antibodies to the group antigen VP6 in 44 samples. Nine samples were selected for virus isolation. Three virus isolates (P375/4, P410/4 and P646/1) were successfully adapted to growth in cell line MA-104. These isolates were allocated to group A rotaviruses based on ELISA, immunoperoxidase test and electropherotype analysis. Electropherotype analysis demonstrated changes during passage in cell line in two of the three isolates. The selected sample P543/1 proved negative in ELISA in a fecal sample. Electropherotype analysis of this sample revealed a "longer" electropherotype profile. The profile was suggestive of group C rotavirus. Rotavirus group C was confirmed by RT-PCR and by sequence analysis in this sample.https://vetmed.agriculturejournals.cz/artkey/vet-200605-0013_isolation-immunochemical-demonstration-of-field-strains-of-porcine-group-a-rotaviruses-and-electrophoretic-ana.phpcell line ma-104electron microscopyimmunoperoxidase testelisamonoclonal antibodyelectropherotypegroup a and c rotavirusrt-pcr |
| spellingShingle | R. Smitalova L. Rodak I. Psikal B. Smid Isolation, immunochemical demonstration of field strains of porcine group A rotaviruses and electrophoretic analysis of RNA segments of group A and C rotaviruses Veterinární Medicína cell line ma-104 electron microscopy immunoperoxidase test elisa monoclonal antibody electropherotype group a and c rotavirus rt-pcr |
| title | Isolation, immunochemical demonstration of field strains of porcine group A rotaviruses and electrophoretic analysis of RNA segments of group A and C rotaviruses |
| title_full | Isolation, immunochemical demonstration of field strains of porcine group A rotaviruses and electrophoretic analysis of RNA segments of group A and C rotaviruses |
| title_fullStr | Isolation, immunochemical demonstration of field strains of porcine group A rotaviruses and electrophoretic analysis of RNA segments of group A and C rotaviruses |
| title_full_unstemmed | Isolation, immunochemical demonstration of field strains of porcine group A rotaviruses and electrophoretic analysis of RNA segments of group A and C rotaviruses |
| title_short | Isolation, immunochemical demonstration of field strains of porcine group A rotaviruses and electrophoretic analysis of RNA segments of group A and C rotaviruses |
| title_sort | isolation immunochemical demonstration of field strains of porcine group a rotaviruses and electrophoretic analysis of rna segments of group a and c rotaviruses |
| topic | cell line ma-104 electron microscopy immunoperoxidase test elisa monoclonal antibody electropherotype group a and c rotavirus rt-pcr |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200605-0013_isolation-immunochemical-demonstration-of-field-strains-of-porcine-group-a-rotaviruses-and-electrophoretic-ana.php |
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