Complex genetic interactions in a quantitative trait locus.
Whether in natural populations or between two unrelated members of a species, most phenotypic variation is quantitative. To analyze such quantitative traits, one must first map the underlying quantitative trait loci. Next, and far more difficult, one must identify the quantitative trait genes (QTGs)...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2006-02-01
|
| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC1359075?pdf=render |
| _version_ | 1818391281316921344 |
|---|---|
| author | Himanshu Sinha Bradly P Nicholson Lars M Steinmetz John H McCusker |
| author_facet | Himanshu Sinha Bradly P Nicholson Lars M Steinmetz John H McCusker |
| author_sort | Himanshu Sinha |
| collection | DOAJ |
| description | Whether in natural populations or between two unrelated members of a species, most phenotypic variation is quantitative. To analyze such quantitative traits, one must first map the underlying quantitative trait loci. Next, and far more difficult, one must identify the quantitative trait genes (QTGs), characterize QTG interactions, and identify the phenotypically relevant polymorphisms to determine how QTGs contribute to phenotype. In this work, we analyzed three Saccharomyces cerevisiae high-temperature growth (Htg) QTGs (MKT1, END3, and RHO2). We observed a high level of genetic interactions among QTGs and strain background. Interestingly, while the MKT1 and END3 coding polymorphisms contribute to phenotype, it is the RHO2 3'UTR polymorphisms that are phenotypically relevant. Reciprocal hemizygosity analysis of the Htg QTGs in hybrids between S288c and ten unrelated S. cerevisiae strains reveals that the contributions of the Htg QTGs are not conserved in nine other hybrids, which has implications for QTG identification by marker-trait association. Our findings demonstrate the variety and complexity of QTG contributions to phenotype, the impact of genetic background, and the value of quantitative genetic studies in S. cerevisiae. |
| first_indexed | 2024-12-14T05:11:02Z |
| format | Article |
| id | doaj.art-1ae478d625a64f1da12a99f5dc68f558 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-14T05:11:02Z |
| publishDate | 2006-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-1ae478d625a64f1da12a99f5dc68f5582022-12-21T23:15:58ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042006-02-0122e1310.1371/journal.pgen.0020013Complex genetic interactions in a quantitative trait locus.Himanshu SinhaBradly P NicholsonLars M SteinmetzJohn H McCuskerWhether in natural populations or between two unrelated members of a species, most phenotypic variation is quantitative. To analyze such quantitative traits, one must first map the underlying quantitative trait loci. Next, and far more difficult, one must identify the quantitative trait genes (QTGs), characterize QTG interactions, and identify the phenotypically relevant polymorphisms to determine how QTGs contribute to phenotype. In this work, we analyzed three Saccharomyces cerevisiae high-temperature growth (Htg) QTGs (MKT1, END3, and RHO2). We observed a high level of genetic interactions among QTGs and strain background. Interestingly, while the MKT1 and END3 coding polymorphisms contribute to phenotype, it is the RHO2 3'UTR polymorphisms that are phenotypically relevant. Reciprocal hemizygosity analysis of the Htg QTGs in hybrids between S288c and ten unrelated S. cerevisiae strains reveals that the contributions of the Htg QTGs are not conserved in nine other hybrids, which has implications for QTG identification by marker-trait association. Our findings demonstrate the variety and complexity of QTG contributions to phenotype, the impact of genetic background, and the value of quantitative genetic studies in S. cerevisiae.http://europepmc.org/articles/PMC1359075?pdf=render |
| spellingShingle | Himanshu Sinha Bradly P Nicholson Lars M Steinmetz John H McCusker Complex genetic interactions in a quantitative trait locus. PLoS Genetics |
| title | Complex genetic interactions in a quantitative trait locus. |
| title_full | Complex genetic interactions in a quantitative trait locus. |
| title_fullStr | Complex genetic interactions in a quantitative trait locus. |
| title_full_unstemmed | Complex genetic interactions in a quantitative trait locus. |
| title_short | Complex genetic interactions in a quantitative trait locus. |
| title_sort | complex genetic interactions in a quantitative trait locus |
| url | http://europepmc.org/articles/PMC1359075?pdf=render |
| work_keys_str_mv | AT himanshusinha complexgeneticinteractionsinaquantitativetraitlocus AT bradlypnicholson complexgeneticinteractionsinaquantitativetraitlocus AT larsmsteinmetz complexgeneticinteractionsinaquantitativetraitlocus AT johnhmccusker complexgeneticinteractionsinaquantitativetraitlocus |