The Release of Substance P From Cultured Dorsal Root Ganglion Neurons Requires the Non-neuronal Cells Around These Neurons
Substance P is known to be released from dorsal root ganglion (DRG) neurons, but the possible involvement of non-neuronal cells in the process of substance P release is not known. During the culture period, the number of surviving DRG neurons, the total substance P content from the culture medium an...
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| Format: | Article |
| Language: | English |
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Elsevier
2007-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S134786131934191X |
| _version_ | 1828390726852935680 |
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| author | He-Bin Tang Yu-Sang Li Yoshihiro Nakata |
| author_facet | He-Bin Tang Yu-Sang Li Yoshihiro Nakata |
| author_sort | He-Bin Tang |
| collection | DOAJ |
| description | Substance P is known to be released from dorsal root ganglion (DRG) neurons, but the possible involvement of non-neuronal cells in the process of substance P release is not known. During the culture period, the number of surviving DRG neurons, the total substance P content from the culture medium and DRG cells, and the total protein of DRG cells were monitored. Both the number of surviving neurons and the total substance P content decreased in a time-dependent manner, whereas the total protein synthesis was increased. The localization of substance P in small-to-medium–sized neurons was further confirmed by immunocytochemistry. Finally, the substance P release levels from the neuronal and non-neuronal enrichments were investigated by radioimmunoassay. Both partially purified DRG neurons and non-neuronal cells exhibited a weaker substance P release response to capsaicin or KCl, relative to unpurified DRG cells. The total substance P content from the partially purified DRG neurons was almost the same as that from the unpurified DRG cells, but much more than that from the partially purified DRG non-neuronal cells. These findings suggest that substance P is released from DRG neurons, and this process should require the coexistence of neurons and non-neuronal cells. Keywords:: capsaicin, dorsal root ganglion (DRG) neuron, non-neuronal cell, substance P content, substance P release |
| first_indexed | 2024-12-10T06:51:01Z |
| format | Article |
| id | doaj.art-1ae8cd152b1e41e2a39e255e87b142f0 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-10T06:51:01Z |
| publishDate | 2007-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-1ae8cd152b1e41e2a39e255e87b142f02022-12-22T01:58:33ZengElsevierJournal of Pharmacological Sciences1347-86132007-01-011053264271The Release of Substance P From Cultured Dorsal Root Ganglion Neurons Requires the Non-neuronal Cells Around These NeuronsHe-Bin Tang0Yu-Sang Li1Yoshihiro Nakata2Department of Pharmacology, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8553, JapanDepartment of Anatomical Pathology, Hiroshima University Hospital, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8551, JapanDepartment of Pharmacology, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8553, Japan; Corresponding author. ynakata@hiroshima-u.ac.jpSubstance P is known to be released from dorsal root ganglion (DRG) neurons, but the possible involvement of non-neuronal cells in the process of substance P release is not known. During the culture period, the number of surviving DRG neurons, the total substance P content from the culture medium and DRG cells, and the total protein of DRG cells were monitored. Both the number of surviving neurons and the total substance P content decreased in a time-dependent manner, whereas the total protein synthesis was increased. The localization of substance P in small-to-medium–sized neurons was further confirmed by immunocytochemistry. Finally, the substance P release levels from the neuronal and non-neuronal enrichments were investigated by radioimmunoassay. Both partially purified DRG neurons and non-neuronal cells exhibited a weaker substance P release response to capsaicin or KCl, relative to unpurified DRG cells. The total substance P content from the partially purified DRG neurons was almost the same as that from the unpurified DRG cells, but much more than that from the partially purified DRG non-neuronal cells. These findings suggest that substance P is released from DRG neurons, and this process should require the coexistence of neurons and non-neuronal cells. Keywords:: capsaicin, dorsal root ganglion (DRG) neuron, non-neuronal cell, substance P content, substance P releasehttp://www.sciencedirect.com/science/article/pii/S134786131934191X |
| spellingShingle | He-Bin Tang Yu-Sang Li Yoshihiro Nakata The Release of Substance P From Cultured Dorsal Root Ganglion Neurons Requires the Non-neuronal Cells Around These Neurons Journal of Pharmacological Sciences |
| title | The Release of Substance P From Cultured Dorsal Root Ganglion Neurons Requires the Non-neuronal Cells Around These Neurons |
| title_full | The Release of Substance P From Cultured Dorsal Root Ganglion Neurons Requires the Non-neuronal Cells Around These Neurons |
| title_fullStr | The Release of Substance P From Cultured Dorsal Root Ganglion Neurons Requires the Non-neuronal Cells Around These Neurons |
| title_full_unstemmed | The Release of Substance P From Cultured Dorsal Root Ganglion Neurons Requires the Non-neuronal Cells Around These Neurons |
| title_short | The Release of Substance P From Cultured Dorsal Root Ganglion Neurons Requires the Non-neuronal Cells Around These Neurons |
| title_sort | release of substance p from cultured dorsal root ganglion neurons requires the non neuronal cells around these neurons |
| url | http://www.sciencedirect.com/science/article/pii/S134786131934191X |
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