Biodegradable Cell Microcarriers Based on Chitosan/Polyester Graft-Copolymers
Self-stabilizing biodegradable microcarriers were produced via an oil/water solvent evaporation technique using amphiphilic chitosan-g-polyester copolymers as a core material in oil phase without the addition of any emulsifier in aqueous phase. The total yield of the copolymer-based microparticles r...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/25/8/1949 |
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| author | Tatiana S. Demina Maria G. Drozdova Chantal Sevrin Philippe Compère Tatiana A. Akopova Elena Markvicheva Christian Grandfils |
| author_facet | Tatiana S. Demina Maria G. Drozdova Chantal Sevrin Philippe Compère Tatiana A. Akopova Elena Markvicheva Christian Grandfils |
| author_sort | Tatiana S. Demina |
| collection | DOAJ |
| description | Self-stabilizing biodegradable microcarriers were produced via an oil/water solvent evaporation technique using amphiphilic chitosan-g-polyester copolymers as a core material in oil phase without the addition of any emulsifier in aqueous phase. The total yield of the copolymer-based microparticles reached up to 79 wt. %, which is comparable to a yield achievable using traditional emulsifiers. The kinetics of microparticle self-stabilization, monitored during their process, were correlated to the migration of hydrophilic copolymer’s moieties to the oil/water interface. With a favorable surface/volume ratio and the presence of bioadhesive natural fragments anchored to their surface, the performance of these novel microcarriers has been highlighted by evaluating cell morphology and proliferation within a week of cell cultivation in vitro. |
| first_indexed | 2024-03-10T20:18:02Z |
| format | Article |
| id | doaj.art-1aecb7c88ae841a4b3ee6be0eb6335b4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:18:02Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1aecb7c88ae841a4b3ee6be0eb6335b42023-11-19T22:25:11ZengMDPI AGMolecules1420-30492020-04-01258194910.3390/molecules25081949Biodegradable Cell Microcarriers Based on Chitosan/Polyester Graft-CopolymersTatiana S. Demina0Maria G. Drozdova1Chantal Sevrin2Philippe Compère3Tatiana A. Akopova4Elena Markvicheva5Christian Grandfils6Enikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences (ISPM RAS), 70 Profsoyuznaya str., 117393 Moscow, RussiaShemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya str., 117997 Moscow, RussiaInterfaculty Research Centre on Biomaterials (CEIB), University of Liège, Chemistry Institute, B6C, 11 Allée du 6 août, B-4000 Liege (Sart-Tilman), BelgiumInterfaculty Research Centre on Biomaterials (CEIB), University of Liège, Chemistry Institute, B6C, 11 Allée du 6 août, B-4000 Liege (Sart-Tilman), BelgiumEnikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences (ISPM RAS), 70 Profsoyuznaya str., 117393 Moscow, RussiaShemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya str., 117997 Moscow, RussiaInterfaculty Research Centre on Biomaterials (CEIB), University of Liège, Chemistry Institute, B6C, 11 Allée du 6 août, B-4000 Liege (Sart-Tilman), BelgiumSelf-stabilizing biodegradable microcarriers were produced via an oil/water solvent evaporation technique using amphiphilic chitosan-g-polyester copolymers as a core material in oil phase without the addition of any emulsifier in aqueous phase. The total yield of the copolymer-based microparticles reached up to 79 wt. %, which is comparable to a yield achievable using traditional emulsifiers. The kinetics of microparticle self-stabilization, monitored during their process, were correlated to the migration of hydrophilic copolymer’s moieties to the oil/water interface. With a favorable surface/volume ratio and the presence of bioadhesive natural fragments anchored to their surface, the performance of these novel microcarriers has been highlighted by evaluating cell morphology and proliferation within a week of cell cultivation in vitro.https://www.mdpi.com/1420-3049/25/8/1949microcarriersgraft-copolymersoil/water emulsiontissue engineeringfibroblastspolylactide |
| spellingShingle | Tatiana S. Demina Maria G. Drozdova Chantal Sevrin Philippe Compère Tatiana A. Akopova Elena Markvicheva Christian Grandfils Biodegradable Cell Microcarriers Based on Chitosan/Polyester Graft-Copolymers Molecules microcarriers graft-copolymers oil/water emulsion tissue engineering fibroblasts polylactide |
| title | Biodegradable Cell Microcarriers Based on Chitosan/Polyester Graft-Copolymers |
| title_full | Biodegradable Cell Microcarriers Based on Chitosan/Polyester Graft-Copolymers |
| title_fullStr | Biodegradable Cell Microcarriers Based on Chitosan/Polyester Graft-Copolymers |
| title_full_unstemmed | Biodegradable Cell Microcarriers Based on Chitosan/Polyester Graft-Copolymers |
| title_short | Biodegradable Cell Microcarriers Based on Chitosan/Polyester Graft-Copolymers |
| title_sort | biodegradable cell microcarriers based on chitosan polyester graft copolymers |
| topic | microcarriers graft-copolymers oil/water emulsion tissue engineering fibroblasts polylactide |
| url | https://www.mdpi.com/1420-3049/25/8/1949 |
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