A study on phenotypic characterization and genotypic determination of drug resistance of Helicobacter pylori from cases of acid peptic disease in a tertiary care center
Background: Helicobacter pylori infection is one of the major risk factors for gastroduodenal disease, and invasive or non-invasive tests are available to detect it. Aims and Objectives: The study aims to identify H. pylori phenotypically and genotypically to identify drug-resistant H. pylori in...
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| Format: | Article |
| Language: | English |
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Manipal College of Medical Sciences, Pokhara
2024-01-01
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| Series: | Asian Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://www.nepjol.info/index.php/AJMS/article/view/57358 |
| _version_ | 1797369504020299776 |
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| author | Palaneswamy Savetha Rajesh Samuel Ajit George Ravin Devasir Sathyaseelan Malini Evangeline Rose. C Noor Mohamed Rasik B |
| author_facet | Palaneswamy Savetha Rajesh Samuel Ajit George Ravin Devasir Sathyaseelan Malini Evangeline Rose. C Noor Mohamed Rasik B |
| author_sort | Palaneswamy Savetha |
| collection | DOAJ |
| description | Background: Helicobacter pylori infection is one of the major risk factors for gastroduodenal disease, and invasive or non-invasive tests are available to detect it.
Aims and Objectives: The study aims to identify H. pylori phenotypically and genotypically to identify drug-resistant H. pylori in suspected cases of peptic acid disorders at a tertiary care center.
Materials and Methods: This 6-month prospective and observational study was performed at Saveetha Medical College and Hospitals. Patients’ gastric biopsy samples were obtained from 105 individuals with gastroduodenal disorders who had upper gastrointestinal endoscopies at the Hospital. A fiber optic endoscope and biopsy forceps were used for the endoscopy. Biopsy samples were collected from the antrum and placed in Eppendorf tubes for fast urease, culture, gram stain, polymerase chain reaction (PCR), and histological analysis.
Results: A total of 78 (74%) males and 27 (26%) females were among the 105 patients. Endoscopic findings imply gastritis, gastric ulcer, and duodenal ulcer in 74 patients (70.48%). Rapid urease test (RUT), gram stain, and histology revealed positive results in 20 cases. RUT showed that 30.47% of patients tested positive. Gram stain detected 25.1% of H. pylori infections, and 12 cases were histopathologically positive. By PCR, 21.62% of gastritis patients were positive for H. pylori infection. RUT and PCR give comparable results for detecting H. pylori infection, with a strong association between H. pylori infection and peptic ulcer disease.
Conclusion: A combination of RUT and PCR is an effective diagnostic approach for H. pylori infection, allowing doctors to select the most appropriate medications for specific patients. |
| first_indexed | 2024-03-08T17:47:44Z |
| format | Article |
| id | doaj.art-1aed4689bbf74eb286c6ab529f07ca40 |
| institution | Directory Open Access Journal |
| issn | 2467-9100 2091-0576 |
| language | English |
| last_indexed | 2024-03-08T17:47:44Z |
| publishDate | 2024-01-01 |
| publisher | Manipal College of Medical Sciences, Pokhara |
| record_format | Article |
| series | Asian Journal of Medical Sciences |
| spelling | doaj.art-1aed4689bbf74eb286c6ab529f07ca402024-01-02T09:55:26ZengManipal College of Medical Sciences, PokharaAsian Journal of Medical Sciences2467-91002091-05762024-01-01151135141https://doi.org/10.3126/ajms.v15i1.57358A study on phenotypic characterization and genotypic determination of drug resistance of Helicobacter pylori from cases of acid peptic disease in a tertiary care centerPalaneswamy Savetha 0https://orcid.org/0000-0002-4908-3092Rajesh Samuel Ajit George 1https://orcid.org/0000-0002-3173-3296Ravin Devasir Sathyaseelan 2https://orcid.org/0000-0003-2381-2098Malini Evangeline Rose. C 3https://orcid.org/0000-0003-3035-9201Noor Mohamed Rasik B 4https://orcid.org/0009-0008-8458-7350Assistant Professor, Department of Microbiology, Tagore Medical College and Hospital, Chennai, Tamil Nadu, India Assistant Professor, Department of Otorhinolaryngology. Sri Sathya Sai Medical College and Research Institute, Puducherry, India Professor, Department of General Medicine, Sri Lalithambigai Medical College and Hospital, Faculty of Medicine, Dr. M.G.R. Educational Research Institute, Chennai, Tamil Nadu, India Assistant Professor, Department of Microbiology, Tagore Medical College and Hospital, Chennai, Tamil Nadu, India Assistant Professor, Department of Community Medicine, Sri Lalithambigai Medical College and Hospital, Faculty of Medicine, Dr. M.G.R. Educational Research Institute, Chennai, Tamil Nadu, India Background: Helicobacter pylori infection is one of the major risk factors for gastroduodenal disease, and invasive or non-invasive tests are available to detect it. Aims and Objectives: The study aims to identify H. pylori phenotypically and genotypically to identify drug-resistant H. pylori in suspected cases of peptic acid disorders at a tertiary care center. Materials and Methods: This 6-month prospective and observational study was performed at Saveetha Medical College and Hospitals. Patients’ gastric biopsy samples were obtained from 105 individuals with gastroduodenal disorders who had upper gastrointestinal endoscopies at the Hospital. A fiber optic endoscope and biopsy forceps were used for the endoscopy. Biopsy samples were collected from the antrum and placed in Eppendorf tubes for fast urease, culture, gram stain, polymerase chain reaction (PCR), and histological analysis. Results: A total of 78 (74%) males and 27 (26%) females were among the 105 patients. Endoscopic findings imply gastritis, gastric ulcer, and duodenal ulcer in 74 patients (70.48%). Rapid urease test (RUT), gram stain, and histology revealed positive results in 20 cases. RUT showed that 30.47% of patients tested positive. Gram stain detected 25.1% of H. pylori infections, and 12 cases were histopathologically positive. By PCR, 21.62% of gastritis patients were positive for H. pylori infection. RUT and PCR give comparable results for detecting H. pylori infection, with a strong association between H. pylori infection and peptic ulcer disease. Conclusion: A combination of RUT and PCR is an effective diagnostic approach for H. pylori infection, allowing doctors to select the most appropriate medications for specific patients.https://www.nepjol.info/index.php/AJMS/article/view/57358helicobacter pylori; drug-resistant; peptic acid; rapid urease test; polymerase chain reaction; gram stain |
| spellingShingle | Palaneswamy Savetha Rajesh Samuel Ajit George Ravin Devasir Sathyaseelan Malini Evangeline Rose. C Noor Mohamed Rasik B A study on phenotypic characterization and genotypic determination of drug resistance of Helicobacter pylori from cases of acid peptic disease in a tertiary care center Asian Journal of Medical Sciences helicobacter pylori; drug-resistant; peptic acid; rapid urease test; polymerase chain reaction; gram stain |
| title | A study on phenotypic characterization and genotypic determination of drug resistance of Helicobacter pylori from cases of acid peptic disease in a tertiary care center |
| title_full | A study on phenotypic characterization and genotypic determination of drug resistance of Helicobacter pylori from cases of acid peptic disease in a tertiary care center |
| title_fullStr | A study on phenotypic characterization and genotypic determination of drug resistance of Helicobacter pylori from cases of acid peptic disease in a tertiary care center |
| title_full_unstemmed | A study on phenotypic characterization and genotypic determination of drug resistance of Helicobacter pylori from cases of acid peptic disease in a tertiary care center |
| title_short | A study on phenotypic characterization and genotypic determination of drug resistance of Helicobacter pylori from cases of acid peptic disease in a tertiary care center |
| title_sort | study on phenotypic characterization and genotypic determination of drug resistance of helicobacter pylori from cases of acid peptic disease in a tertiary care center |
| topic | helicobacter pylori; drug-resistant; peptic acid; rapid urease test; polymerase chain reaction; gram stain |
| url | https://www.nepjol.info/index.php/AJMS/article/view/57358 |
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