Deletion of alpB Gene Influences Outer Membrane Vesicles Biogenesis of Lysobacter sp. XL1
Outer membrane vesicles (OMVs) produced by Gram-negative bacteria constitute important factors in defining interactions with the extracellular milieu. Lysobacter sp. XL1 produces OMVs capable of lysing microbial cells due to the presence in their cargo of bacteriolytic protease L5 (AlpB). Although p...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.715802/full |
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| author | Irina V. Kudryakova Alexey S. Afoshin Tanya V. Ivashina Natalia E. Suzina Elena A. Leontyevskaya Natalia V. Leontyevskaya (Vasilyeva) |
| author_facet | Irina V. Kudryakova Alexey S. Afoshin Tanya V. Ivashina Natalia E. Suzina Elena A. Leontyevskaya Natalia V. Leontyevskaya (Vasilyeva) |
| author_sort | Irina V. Kudryakova |
| collection | DOAJ |
| description | Outer membrane vesicles (OMVs) produced by Gram-negative bacteria constitute important factors in defining interactions with the extracellular milieu. Lysobacter sp. XL1 produces OMVs capable of lysing microbial cells due to the presence in their cargo of bacteriolytic protease L5 (AlpB). Although protein L5 has been functionally and biochemically characterized (including aspects of its packing into OMVs), its role in vesicle biogenesis through genetic deletion of alpB had not been studied previously. Here, we have successfully deleted alpB by allelic replacement and show that the alpB deletion mutant produces a significantly lower amount of OMVs that lack bacteriolytic activity and display altered ultrastructural characteristics in relation to the OMVs produced by the wild-type strain. These results confirm that, as previously proposed, protein L5 participates in OMV production through a mechanism that is not yet fully understood. |
| first_indexed | 2024-12-13T21:42:42Z |
| format | Article |
| id | doaj.art-1aee4d23541f4df0ad55effbc05892b4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T21:42:42Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1aee4d23541f4df0ad55effbc05892b42022-12-21T23:30:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-08-011210.3389/fmicb.2021.715802715802Deletion of alpB Gene Influences Outer Membrane Vesicles Biogenesis of Lysobacter sp. XL1Irina V. Kudryakova0Alexey S. Afoshin1Tanya V. Ivashina2Natalia E. Suzina3Elena A. Leontyevskaya4Natalia V. Leontyevskaya (Vasilyeva)5Laboratory of Microbial Cell Surface Biochemistry, Pushchino Center for Biological Research, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, RussiaLaboratory of Microbial Cell Surface Biochemistry, Pushchino Center for Biological Research, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, RussiaLaboratory of Molecular Microbiology, Pushchino Center for Biological Research, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, RussiaLaboratory of Microbial Cytology, Pushchino Center for Biological Research, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, RussiaLaboratory of Microbial Cell Surface Biochemistry, Pushchino Center for Biological Research, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, RussiaLaboratory of Microbial Cell Surface Biochemistry, Pushchino Center for Biological Research, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, RussiaOuter membrane vesicles (OMVs) produced by Gram-negative bacteria constitute important factors in defining interactions with the extracellular milieu. Lysobacter sp. XL1 produces OMVs capable of lysing microbial cells due to the presence in their cargo of bacteriolytic protease L5 (AlpB). Although protein L5 has been functionally and biochemically characterized (including aspects of its packing into OMVs), its role in vesicle biogenesis through genetic deletion of alpB had not been studied previously. Here, we have successfully deleted alpB by allelic replacement and show that the alpB deletion mutant produces a significantly lower amount of OMVs that lack bacteriolytic activity and display altered ultrastructural characteristics in relation to the OMVs produced by the wild-type strain. These results confirm that, as previously proposed, protein L5 participates in OMV production through a mechanism that is not yet fully understood.https://www.frontiersin.org/articles/10.3389/fmicb.2021.715802/fullbiogenesis of vesiclesLysobacter sp. XL1antimicrobial potency of vesiclesdeletion in gene alpBbacteriolytic protease L5 |
| spellingShingle | Irina V. Kudryakova Alexey S. Afoshin Tanya V. Ivashina Natalia E. Suzina Elena A. Leontyevskaya Natalia V. Leontyevskaya (Vasilyeva) Deletion of alpB Gene Influences Outer Membrane Vesicles Biogenesis of Lysobacter sp. XL1 Frontiers in Microbiology biogenesis of vesicles Lysobacter sp. XL1 antimicrobial potency of vesicles deletion in gene alpB bacteriolytic protease L5 |
| title | Deletion of alpB Gene Influences Outer Membrane Vesicles Biogenesis of Lysobacter sp. XL1 |
| title_full | Deletion of alpB Gene Influences Outer Membrane Vesicles Biogenesis of Lysobacter sp. XL1 |
| title_fullStr | Deletion of alpB Gene Influences Outer Membrane Vesicles Biogenesis of Lysobacter sp. XL1 |
| title_full_unstemmed | Deletion of alpB Gene Influences Outer Membrane Vesicles Biogenesis of Lysobacter sp. XL1 |
| title_short | Deletion of alpB Gene Influences Outer Membrane Vesicles Biogenesis of Lysobacter sp. XL1 |
| title_sort | deletion of alpb gene influences outer membrane vesicles biogenesis of lysobacter sp xl1 |
| topic | biogenesis of vesicles Lysobacter sp. XL1 antimicrobial potency of vesicles deletion in gene alpB bacteriolytic protease L5 |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.715802/full |
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