Effect of cardiopulmonary bypass on annexin A1 expression in peripheral blood mononuclear cells of children with congenital heart disease
This study aimed to investigate the effect of cardiopulmonary bypass (CPB) on Annexin A1 expression in the peripheral blood mononuclear cells (PBMCs) of children with congenital heart disease (CHD). A total of 30 children receiving CPB for interventricular septal defect were included. Peripheral blo...
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| Format: | Article |
| Language: | English |
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Society of Medical Biochemists of Serbia, Belgrade
2012-01-01
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| Series: | Journal of Medical Biochemistry |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2012/1452-82581203193Z.pdf |
| _version_ | 1798043607948591104 |
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| author | Zhou Gengxu Hong Xiaoyang Liu Yuhang Feng Zhichun |
| author_facet | Zhou Gengxu Hong Xiaoyang Liu Yuhang Feng Zhichun |
| author_sort | Zhou Gengxu |
| collection | DOAJ |
| description | This study aimed to investigate the effect of cardiopulmonary bypass (CPB) on Annexin A1 expression in the peripheral blood mononuclear cells (PBMCs) of children with congenital heart disease (CHD). A total of 30 children receiving CPB for interventricular septal defect were included. Peripheral blood was collected before and after CPB. PBMCs were collected by density gradient centrifugation. Protein extraction was performed by lysis and subjected to 2DQUANT for protein quantitation. Isoelectric focusing electrophoresis (IEF) was carried out followed by gel image analysis. Protein spots with a difference in expression of >1.5 fold were collected as candidate proteins which were subjected to mass spectrometry for the identification of differentially expressed proteins. Western blot assay was employed to confirm the expressions of target proteins. Peripheral blood collected at two time points was subjected to two-dimensional electrophoresis, and a total of 12 differentially expressed proteins were identified. Of them, 5 proteins had decreased expression before CPB (T0) but their expressions increased after CPB (T1); the remaining 7 proteins had in creased expressions before CPB but their expressions reduced after CPB. One of these differentially expressed proteins was Annexin A1. Western blot assay confirmed that Annexin A1 expression began to increase at 0.5 h after CPB, and the increase of Annexin A1 was more obvious after CPB. Our findings primarily indicate the potential mechanism underlying the role of PBMC in inflammatory response following CPB, and provide a target for the prevention and control of post-CPB systemic inflammatory response syndrome (SIRS). |
| first_indexed | 2024-04-11T22:51:29Z |
| format | Article |
| id | doaj.art-1aee8554ce1d4167b453cc4d36348d55 |
| institution | Directory Open Access Journal |
| issn | 1452-8258 1452-8266 |
| language | English |
| last_indexed | 2024-04-11T22:51:29Z |
| publishDate | 2012-01-01 |
| publisher | Society of Medical Biochemists of Serbia, Belgrade |
| record_format | Article |
| series | Journal of Medical Biochemistry |
| spelling | doaj.art-1aee8554ce1d4167b453cc4d36348d552022-12-22T03:58:34ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662012-01-013131931981452-82581203193ZEffect of cardiopulmonary bypass on annexin A1 expression in peripheral blood mononuclear cells of children with congenital heart diseaseZhou Gengxu0Hong Xiaoyang1Liu Yuhang2Feng Zhichun3Affiliated Bayi Children Hospital, Beijing Military General Hospital, Beijing, ChinaAffiliated Bayi Children Hospital, Beijing Military General Hospital, Beijing, China + Affiliated Bayi Children Hospital, Bayi Clinical Medical Institute, South Medical University, ChinaAffiliated Bayi Children Hospital, Beijing Military General Hospital, Beijing, ChinaAffiliated Bayi Children Hospital, Beijing Military General Hospital, Beijing, ChinaThis study aimed to investigate the effect of cardiopulmonary bypass (CPB) on Annexin A1 expression in the peripheral blood mononuclear cells (PBMCs) of children with congenital heart disease (CHD). A total of 30 children receiving CPB for interventricular septal defect were included. Peripheral blood was collected before and after CPB. PBMCs were collected by density gradient centrifugation. Protein extraction was performed by lysis and subjected to 2DQUANT for protein quantitation. Isoelectric focusing electrophoresis (IEF) was carried out followed by gel image analysis. Protein spots with a difference in expression of >1.5 fold were collected as candidate proteins which were subjected to mass spectrometry for the identification of differentially expressed proteins. Western blot assay was employed to confirm the expressions of target proteins. Peripheral blood collected at two time points was subjected to two-dimensional electrophoresis, and a total of 12 differentially expressed proteins were identified. Of them, 5 proteins had decreased expression before CPB (T0) but their expressions increased after CPB (T1); the remaining 7 proteins had in creased expressions before CPB but their expressions reduced after CPB. One of these differentially expressed proteins was Annexin A1. Western blot assay confirmed that Annexin A1 expression began to increase at 0.5 h after CPB, and the increase of Annexin A1 was more obvious after CPB. Our findings primarily indicate the potential mechanism underlying the role of PBMC in inflammatory response following CPB, and provide a target for the prevention and control of post-CPB systemic inflammatory response syndrome (SIRS).https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2012/1452-82581203193Z.pdfannexin a1cardiopulmonary bypassperipheralblood mononuclear cellscongenital heart disease |
| spellingShingle | Zhou Gengxu Hong Xiaoyang Liu Yuhang Feng Zhichun Effect of cardiopulmonary bypass on annexin A1 expression in peripheral blood mononuclear cells of children with congenital heart disease Journal of Medical Biochemistry annexin a1 cardiopulmonary bypass peripheralblood mononuclear cells congenital heart disease |
| title | Effect of cardiopulmonary bypass on annexin A1 expression in peripheral blood mononuclear cells of children with congenital heart disease |
| title_full | Effect of cardiopulmonary bypass on annexin A1 expression in peripheral blood mononuclear cells of children with congenital heart disease |
| title_fullStr | Effect of cardiopulmonary bypass on annexin A1 expression in peripheral blood mononuclear cells of children with congenital heart disease |
| title_full_unstemmed | Effect of cardiopulmonary bypass on annexin A1 expression in peripheral blood mononuclear cells of children with congenital heart disease |
| title_short | Effect of cardiopulmonary bypass on annexin A1 expression in peripheral blood mononuclear cells of children with congenital heart disease |
| title_sort | effect of cardiopulmonary bypass on annexin a1 expression in peripheral blood mononuclear cells of children with congenital heart disease |
| topic | annexin a1 cardiopulmonary bypass peripheralblood mononuclear cells congenital heart disease |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2012/1452-82581203193Z.pdf |
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