Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil
A cross-sectional analysis of stored Ziehl-Neelsen (ZN)-stained sputum smear slides (SSS) obtained from two public tuberculosis referral laboratories located in Juiz de Fora, Minas Gerais, was carried out to distinguish Mycobacterium bovis from other members of the Mycobacterium tuberculosis complex...
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Fundação Oswaldo Cruz (FIOCRUZ)
2011-02-01
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Series: | Memorias do Instituto Oswaldo Cruz |
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author | Marcio Roberto Silva Mark Drew Crosland Guimarães Vania Maria de Oliveira Aline dos Santos Moreira Ronaldo Rodrigues da Costa Kelly Cristina Ferreira Abi-Zaid Adalgiza da Silva Rocha Philip Noel Suffys |
author_facet | Marcio Roberto Silva Mark Drew Crosland Guimarães Vania Maria de Oliveira Aline dos Santos Moreira Ronaldo Rodrigues da Costa Kelly Cristina Ferreira Abi-Zaid Adalgiza da Silva Rocha Philip Noel Suffys |
author_sort | Marcio Roberto Silva |
collection | DOAJ |
description | A cross-sectional analysis of stored Ziehl-Neelsen (ZN)-stained sputum smear slides (SSS) obtained from two public tuberculosis referral laboratories located in Juiz de Fora, Minas Gerais, was carried out to distinguish Mycobacterium bovis from other members of the Mycobacterium tuberculosis complex (MTC). A two-step approach was used to distinguish M. bovis from other members of MTC: (i) oxyR pseudogene amplification to detect MTC and, subsequently, (ii) allele-specific sequencing based on the polymorphism at position 285 of this gene. The oxyR pseudogene was successfully amplified in 100 of 177 (56.5%) SSS available from 99 individuals. No molecular profile of M. bovis was found. Multivariate analysis indicated that acid-fast bacilli (AFB) results and the source laboratory were associated (p < 0.05) with oxyR pseudogene amplification. SSS that were AFB++ SSS showed more oxyR pseudogene amplification than those with AFB0, possibly due to the amount of DNA. One of the two source laboratories presented a greater chance of oxyR pseudogene amplification, suggesting that differences in sputum conservation between laboratories could have influenced the preservation of DNA. This study provides evidence that stored ZN-SSS can be used for the molecular detection of MTC. |
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institution | Directory Open Access Journal |
issn | 1678-8060 |
language | English |
last_indexed | 2024-03-12T08:46:32Z |
publishDate | 2011-02-01 |
publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
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series | Memorias do Instituto Oswaldo Cruz |
spelling | doaj.art-1aef1d27567140cb920266bf66e02f952023-09-02T16:26:06ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602011-02-011061091510.1590/S0074-02762011000100002S0074-02762011000100002Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in BrazilMarcio Roberto Silva0Mark Drew Crosland Guimarães1Vania Maria de Oliveira2Aline dos Santos Moreira3Ronaldo Rodrigues da Costa4Kelly Cristina Ferreira Abi-Zaid5Adalgiza da Silva Rocha6Philip Noel Suffys7Ministério da Agricultura, Pecuária e AbastecimentoUniversidade Federal de Minas GeraisMinistério da Agricultura, Pecuária e AbastecimentoLaboratório de Genômica Funcional e BioinformáticaUniversidade Federal de Juiz de ForaFundação Hospitalar do Estado de Minas GeraisFundação Oswaldo CruzFundação Oswaldo CruzA cross-sectional analysis of stored Ziehl-Neelsen (ZN)-stained sputum smear slides (SSS) obtained from two public tuberculosis referral laboratories located in Juiz de Fora, Minas Gerais, was carried out to distinguish Mycobacterium bovis from other members of the Mycobacterium tuberculosis complex (MTC). A two-step approach was used to distinguish M. bovis from other members of MTC: (i) oxyR pseudogene amplification to detect MTC and, subsequently, (ii) allele-specific sequencing based on the polymorphism at position 285 of this gene. The oxyR pseudogene was successfully amplified in 100 of 177 (56.5%) SSS available from 99 individuals. No molecular profile of M. bovis was found. Multivariate analysis indicated that acid-fast bacilli (AFB) results and the source laboratory were associated (p < 0.05) with oxyR pseudogene amplification. SSS that were AFB++ SSS showed more oxyR pseudogene amplification than those with AFB0, possibly due to the amount of DNA. One of the two source laboratories presented a greater chance of oxyR pseudogene amplification, suggesting that differences in sputum conservation between laboratories could have influenced the preservation of DNA. This study provides evidence that stored ZN-SSS can be used for the molecular detection of MTC.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000100002&lng=en&tlng=enoxyR pseudogenepolymerase chain reactionsputum smearMycobacterium tuberculosisMycobacterium bovismolecular sequence data |
spellingShingle | Marcio Roberto Silva Mark Drew Crosland Guimarães Vania Maria de Oliveira Aline dos Santos Moreira Ronaldo Rodrigues da Costa Kelly Cristina Ferreira Abi-Zaid Adalgiza da Silva Rocha Philip Noel Suffys Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil Memorias do Instituto Oswaldo Cruz oxyR pseudogene polymerase chain reaction sputum smear Mycobacterium tuberculosis Mycobacterium bovis molecular sequence data |
title | Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil |
title_full | Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil |
title_fullStr | Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil |
title_full_unstemmed | Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil |
title_short | Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil |
title_sort | identification of mycobacterium tuberculosis complex based on amplification and sequencing of the oxyr pseudogene from stored ziehl neelsen stained sputum smears in brazil |
topic | oxyR pseudogene polymerase chain reaction sputum smear Mycobacterium tuberculosis Mycobacterium bovis molecular sequence data |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000100002&lng=en&tlng=en |
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