Improving the yield of Anoectochilus roxburghii by Bacillus licheniformis cultured in Agaricus bisporus industrial wastewater
Background: There is a large amount of industrial wastewater produced by the mushroom industry during the canning processing each year, which could provide abundant carbon, nitrogen and inorganic salts for microbial growth. The aim of this study was to optimize the culture conditions for Bacillus li...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-11-01
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| Series: | Electronic Journal of Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345820300397 |
| _version_ | 1819172989250306048 |
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| author | Jiafu Huang Axian Huang Luanmei Lu Weikang Jiang Danfeng Zhang Qici Wu Peng Li Xiuyan Zhong |
| author_facet | Jiafu Huang Axian Huang Luanmei Lu Weikang Jiang Danfeng Zhang Qici Wu Peng Li Xiuyan Zhong |
| author_sort | Jiafu Huang |
| collection | DOAJ |
| description | Background: There is a large amount of industrial wastewater produced by the mushroom industry during the canning processing each year, which could provide abundant carbon, nitrogen and inorganic salts for microbial growth. The aim of this study was to optimize the culture conditions for Bacillus licheniformis cultured in the Agaricus bisporus industrial wastewater to produce the agricultural microbial fertilizer. Results: In this work, the maximal biomass of B. licheniformis could be obtained under the following culture conditions: 33.7°C, pH 7.0, 221 rpm shaking speed, 0.5% wastewater, 2 (v:v, %) inoculum dose, loading liquid of 60 mL/250 mL and a culture time of 24 h, and the average experimental value obtained was 1.35 ± 0.04 × 109 Obj/mL, which was within the 95% confidence interval of the predicted model (1.29–1.38 × 109 Obj/mL), and met the national microbial fertilizers' standard in China. Furthermore, the field experiment results showed that the fermentation broth of B. licheniformis could significantly improve the yield of Anoectochilus roxburghii. Conclusions: Agaricus bisporus industrial wastewater can be used to produce agricultural microbial fertilizer.How to cite: Huang J, Huang A, Lu L, et al. Improving the yield of Anoectochilus roxburghii by Bacillus licheniformis cultured in Agaricus bisporus industrial wastewater. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.08.002. |
| first_indexed | 2024-12-22T20:15:56Z |
| format | Article |
| id | doaj.art-1af727631a8947c7b44c4cc75c83bf7d |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-22T20:15:56Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-1af727631a8947c7b44c4cc75c83bf7d2022-12-21T18:13:58ZengElsevierElectronic Journal of Biotechnology0717-34582020-11-01481322Improving the yield of Anoectochilus roxburghii by Bacillus licheniformis cultured in Agaricus bisporus industrial wastewaterJiafu Huang0Axian Huang1Luanmei Lu2Weikang Jiang3Danfeng Zhang4Qici Wu5Peng Li6Xiuyan Zhong7Collaborative Innovation Center of Mushroom Health Industry, Minnan Normal University, Zhangzhou, Fujian, China; Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, Fujian, China; School of Life Sciences & Biotechnology College, Minnan Normal University, Zhangzhou, Fujian, China; Corresponding author.Zhangzhou MEILIDE Biological Co., Ltd., Zhangzhou, Fujian, ChinaSchool of Life Sciences & Biotechnology College, Minnan Normal University, Zhangzhou, Fujian, ChinaZhangzhou MEILIDE Biological Co., Ltd., Zhangzhou, Fujian, ChinaSchool of Life Sciences & Biotechnology College, Minnan Normal University, Zhangzhou, Fujian, ChinaCollaborative Innovation Center of Mushroom Health Industry, Minnan Normal University, Zhangzhou, Fujian, China; Engineering Technological Center of Mushroom Industry, Minnan Normal University, Zhangzhou, Fujian, China; School of Life Sciences & Biotechnology College, Minnan Normal University, Zhangzhou, Fujian, ChinaSchool of Life Sciences & Biotechnology College, Minnan Normal University, Zhangzhou, Fujian, ChinaSchool of Life Sciences & Biotechnology College, Minnan Normal University, Zhangzhou, Fujian, ChinaBackground: There is a large amount of industrial wastewater produced by the mushroom industry during the canning processing each year, which could provide abundant carbon, nitrogen and inorganic salts for microbial growth. The aim of this study was to optimize the culture conditions for Bacillus licheniformis cultured in the Agaricus bisporus industrial wastewater to produce the agricultural microbial fertilizer. Results: In this work, the maximal biomass of B. licheniformis could be obtained under the following culture conditions: 33.7°C, pH 7.0, 221 rpm shaking speed, 0.5% wastewater, 2 (v:v, %) inoculum dose, loading liquid of 60 mL/250 mL and a culture time of 24 h, and the average experimental value obtained was 1.35 ± 0.04 × 109 Obj/mL, which was within the 95% confidence interval of the predicted model (1.29–1.38 × 109 Obj/mL), and met the national microbial fertilizers' standard in China. Furthermore, the field experiment results showed that the fermentation broth of B. licheniformis could significantly improve the yield of Anoectochilus roxburghii. Conclusions: Agaricus bisporus industrial wastewater can be used to produce agricultural microbial fertilizer.How to cite: Huang J, Huang A, Lu L, et al. Improving the yield of Anoectochilus roxburghii by Bacillus licheniformis cultured in Agaricus bisporus industrial wastewater. Electron J Biotechnol 2020;48. https://doi.org/10.1016/j.ejbt.2020.08.002.http://www.sciencedirect.com/science/article/pii/S0717345820300397Agaricus bisporusAnoectochilus roxburghiiBacillus licheniformisBox–BehnkenFlow cytometryIndustrial wastewater |
| spellingShingle | Jiafu Huang Axian Huang Luanmei Lu Weikang Jiang Danfeng Zhang Qici Wu Peng Li Xiuyan Zhong Improving the yield of Anoectochilus roxburghii by Bacillus licheniformis cultured in Agaricus bisporus industrial wastewater Electronic Journal of Biotechnology Agaricus bisporus Anoectochilus roxburghii Bacillus licheniformis Box–Behnken Flow cytometry Industrial wastewater |
| title | Improving the yield of Anoectochilus roxburghii by Bacillus licheniformis cultured in Agaricus bisporus industrial wastewater |
| title_full | Improving the yield of Anoectochilus roxburghii by Bacillus licheniformis cultured in Agaricus bisporus industrial wastewater |
| title_fullStr | Improving the yield of Anoectochilus roxburghii by Bacillus licheniformis cultured in Agaricus bisporus industrial wastewater |
| title_full_unstemmed | Improving the yield of Anoectochilus roxburghii by Bacillus licheniformis cultured in Agaricus bisporus industrial wastewater |
| title_short | Improving the yield of Anoectochilus roxburghii by Bacillus licheniformis cultured in Agaricus bisporus industrial wastewater |
| title_sort | improving the yield of anoectochilus roxburghii by bacillus licheniformis cultured in agaricus bisporus industrial wastewater |
| topic | Agaricus bisporus Anoectochilus roxburghii Bacillus licheniformis Box–Behnken Flow cytometry Industrial wastewater |
| url | http://www.sciencedirect.com/science/article/pii/S0717345820300397 |
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