Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen Source

In this study, the determination of the efficiency of a pilot-scale fermentation process using corn steep liquor as a nitrogen source was attempted in order to produce lactic acid via batch culturing of Lactobacillus sp. RKY2. Using pure glucose, fermentation efficiency characteristics, such as fina...

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Main Authors: Young-Jung Wee, Hyang-Ok Kim, Jong-Sun Yun, Hwa-Won Ryu
Format: Article
Language:English
Published: University of Zagreb Faculty of Food Technology and Biotechnology 2006-01-01
Series:Food Technology and Biotechnology
Subjects:
Online Access:http://hrcak.srce.hr/file/162012
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author Young-Jung Wee
Hyang-Ok Kim
Jong-Sun Yun
Hwa-Won Ryu
author_facet Young-Jung Wee
Hyang-Ok Kim
Jong-Sun Yun
Hwa-Won Ryu
author_sort Young-Jung Wee
collection DOAJ
description In this study, the determination of the efficiency of a pilot-scale fermentation process using corn steep liquor as a nitrogen source was attempted in order to produce lactic acid via batch culturing of Lactobacillus sp. RKY2. Using pure glucose, fermentation efficiency characteristics, such as final lactic acid, cell growth, yield, and productivity were not substantially influenced by the scale-up of the laboratory-scale fermentation from 2.5- to 30- and 300-litre scale fermentations. In all experiments, the content of lactic acid produced increased in a linear fashion with increases in the initial glucose concentration. In the experiments using wood hydrolyzate, both lactic acid productivity and cell growth were decreased as a result of the scaling-up of the fermentation. This might be attributed to the toxic chemicals contained in the wood hydrolyzates. However, in all experiments, lactic acid yields remained higher than 90 % with regard to the amount of glucose consumed. Therefore, lactic acid was successfully produced by the pilot-scale bioreactor scheme adopted in this study.
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spelling doaj.art-1af769f362b84bd7bc897aff2642acf92023-12-03T01:38:45ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062006-01-01442293298Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen SourceYoung-Jung Wee0Hyang-Ok Kim1Jong-Sun Yun2Hwa-Won Ryu3School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of KoreaDepartment of Material Chemical and Biochemical Engineering, Chonnam National University, Gwangju 500-757, Republic of KoreaBioHelix, Noan-myeon, Naju, Jeonnam 520-811, Republic of KoreaSchool of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of KoreaIn this study, the determination of the efficiency of a pilot-scale fermentation process using corn steep liquor as a nitrogen source was attempted in order to produce lactic acid via batch culturing of Lactobacillus sp. RKY2. Using pure glucose, fermentation efficiency characteristics, such as final lactic acid, cell growth, yield, and productivity were not substantially influenced by the scale-up of the laboratory-scale fermentation from 2.5- to 30- and 300-litre scale fermentations. In all experiments, the content of lactic acid produced increased in a linear fashion with increases in the initial glucose concentration. In the experiments using wood hydrolyzate, both lactic acid productivity and cell growth were decreased as a result of the scaling-up of the fermentation. This might be attributed to the toxic chemicals contained in the wood hydrolyzates. However, in all experiments, lactic acid yields remained higher than 90 % with regard to the amount of glucose consumed. Therefore, lactic acid was successfully produced by the pilot-scale bioreactor scheme adopted in this study.http://hrcak.srce.hr/file/162012lactic acidLactobacilluscorn steep liquorfermentationwood hydrolyzatepilot-scale
spellingShingle Young-Jung Wee
Hyang-Ok Kim
Jong-Sun Yun
Hwa-Won Ryu
Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen Source
Food Technology and Biotechnology
lactic acid
Lactobacillus
corn steep liquor
fermentation
wood hydrolyzate
pilot-scale
title Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen Source
title_full Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen Source
title_fullStr Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen Source
title_full_unstemmed Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen Source
title_short Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen Source
title_sort pilot scale lactic acid production via batch culturing of lactobacillus sp rky2 using corn steep liquor as a nitrogen source
topic lactic acid
Lactobacillus
corn steep liquor
fermentation
wood hydrolyzate
pilot-scale
url http://hrcak.srce.hr/file/162012
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