Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen Source
In this study, the determination of the efficiency of a pilot-scale fermentation process using corn steep liquor as a nitrogen source was attempted in order to produce lactic acid via batch culturing of Lactobacillus sp. RKY2. Using pure glucose, fermentation efficiency characteristics, such as fina...
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Format: | Article |
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University of Zagreb Faculty of Food Technology and Biotechnology
2006-01-01
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Series: | Food Technology and Biotechnology |
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Online Access: | http://hrcak.srce.hr/file/162012 |
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author | Young-Jung Wee Hyang-Ok Kim Jong-Sun Yun Hwa-Won Ryu |
author_facet | Young-Jung Wee Hyang-Ok Kim Jong-Sun Yun Hwa-Won Ryu |
author_sort | Young-Jung Wee |
collection | DOAJ |
description | In this study, the determination of the efficiency of a pilot-scale fermentation process using corn steep liquor as a nitrogen source was attempted in order to produce lactic acid via batch culturing of Lactobacillus sp. RKY2. Using pure glucose, fermentation efficiency characteristics, such as final lactic acid, cell growth, yield, and productivity were not substantially influenced by the scale-up of the laboratory-scale fermentation from 2.5- to 30- and 300-litre scale fermentations. In all experiments, the content of lactic acid produced increased in a linear fashion with increases in the initial glucose concentration. In the experiments using wood hydrolyzate, both lactic acid productivity and cell growth were decreased as a result of the scaling-up of the fermentation. This might be attributed to the toxic chemicals contained in the wood hydrolyzates. However, in all experiments, lactic acid yields remained higher than 90 % with regard to the amount of glucose consumed. Therefore, lactic acid was successfully produced by the pilot-scale bioreactor scheme adopted in this study. |
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institution | Directory Open Access Journal |
issn | 1330-9862 1334-2606 |
language | English |
last_indexed | 2024-03-09T07:51:23Z |
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series | Food Technology and Biotechnology |
spelling | doaj.art-1af769f362b84bd7bc897aff2642acf92023-12-03T01:38:45ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062006-01-01442293298Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen SourceYoung-Jung Wee0Hyang-Ok Kim1Jong-Sun Yun2Hwa-Won Ryu3School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of KoreaDepartment of Material Chemical and Biochemical Engineering, Chonnam National University, Gwangju 500-757, Republic of KoreaBioHelix, Noan-myeon, Naju, Jeonnam 520-811, Republic of KoreaSchool of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Republic of KoreaIn this study, the determination of the efficiency of a pilot-scale fermentation process using corn steep liquor as a nitrogen source was attempted in order to produce lactic acid via batch culturing of Lactobacillus sp. RKY2. Using pure glucose, fermentation efficiency characteristics, such as final lactic acid, cell growth, yield, and productivity were not substantially influenced by the scale-up of the laboratory-scale fermentation from 2.5- to 30- and 300-litre scale fermentations. In all experiments, the content of lactic acid produced increased in a linear fashion with increases in the initial glucose concentration. In the experiments using wood hydrolyzate, both lactic acid productivity and cell growth were decreased as a result of the scaling-up of the fermentation. This might be attributed to the toxic chemicals contained in the wood hydrolyzates. However, in all experiments, lactic acid yields remained higher than 90 % with regard to the amount of glucose consumed. Therefore, lactic acid was successfully produced by the pilot-scale bioreactor scheme adopted in this study.http://hrcak.srce.hr/file/162012lactic acidLactobacilluscorn steep liquorfermentationwood hydrolyzatepilot-scale |
spellingShingle | Young-Jung Wee Hyang-Ok Kim Jong-Sun Yun Hwa-Won Ryu Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen Source Food Technology and Biotechnology lactic acid Lactobacillus corn steep liquor fermentation wood hydrolyzate pilot-scale |
title | Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen Source |
title_full | Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen Source |
title_fullStr | Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen Source |
title_full_unstemmed | Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen Source |
title_short | Pilot-Scale Lactic Acid Production via Batch Culturing of Lactobacillus sp. RKY2 Using Corn Steep Liquor As a Nitrogen Source |
title_sort | pilot scale lactic acid production via batch culturing of lactobacillus sp rky2 using corn steep liquor as a nitrogen source |
topic | lactic acid Lactobacillus corn steep liquor fermentation wood hydrolyzate pilot-scale |
url | http://hrcak.srce.hr/file/162012 |
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