ZYG11A Is Expressed in Epithelial Ovarian Cancer and Correlates With Low Grade Disease
The insulin-like growth factors (IGF) are important players in the development of gynecological malignancies, including epithelial ovarian cancer (EOC). The identification of biomarkers that can help in the diagnosis and scoring of EOC patients is of fundamental importance in clinical oncology. We h...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2021.688104/full |
| _version_ | 1819260704917553152 |
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| author | Laris Achlaug Lina Somri-Gannam Lina Somri-Gannam Shilhav Meisel-Sharon Shilhav Meisel-Sharon Rive Sarfstein Manisha Dixit Shoshana Yakar Mordechai Hallak Mordechai Hallak Zvi Laron Haim Werner Ilan Bruchim Ilan Bruchim |
| author_facet | Laris Achlaug Lina Somri-Gannam Lina Somri-Gannam Shilhav Meisel-Sharon Shilhav Meisel-Sharon Rive Sarfstein Manisha Dixit Shoshana Yakar Mordechai Hallak Mordechai Hallak Zvi Laron Haim Werner Ilan Bruchim Ilan Bruchim |
| author_sort | Laris Achlaug |
| collection | DOAJ |
| description | The insulin-like growth factors (IGF) are important players in the development of gynecological malignancies, including epithelial ovarian cancer (EOC). The identification of biomarkers that can help in the diagnosis and scoring of EOC patients is of fundamental importance in clinical oncology. We have recently identified the ZYG11A gene as a new candidate target of IGF1 action. The aim of the present study was to evaluate the expression of ZYG11A in EOC patients and to correlate its pattern of expression with histological grade and pathological stage. Furthermore, and in view of previous analyses showing an interplay between ZYG11A, p53 and the IGF1 receptor (IGF1R), we assessed a potential coordinated expression of these proteins in EOC. In addition, zyg11a expression was assessed in ovaries and uteri of growth hormone receptor (GHR) knock-out mice. Tissue microarray analysis was conducted on 36 patients with EOC and expression of ZYG11A, IGF1R and p53 was assessed by immunohistochemistry. Expression levels were correlated with clinical parameters. qPCR was employed to assess zyg11a mRNA levels in mice tissues. Our analyses provide evidence of reduced ZYG11A expression in high grade tumors, consistent with a putative tumor suppressor role. In addition, an inverse correlation between ZYG11A and p53 levels in individual tumors was noticed. Taken together, our data justify further exploration of the role of ZYG11A as a novel biomarker in EOC. |
| first_indexed | 2024-12-23T19:30:09Z |
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| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-23T19:30:09Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-1afbe2b029f3461694ba8b14108ce8cf2022-12-21T17:33:57ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922021-06-011210.3389/fendo.2021.688104688104ZYG11A Is Expressed in Epithelial Ovarian Cancer and Correlates With Low Grade DiseaseLaris Achlaug0Lina Somri-Gannam1Lina Somri-Gannam2Shilhav Meisel-Sharon3Shilhav Meisel-Sharon4Rive Sarfstein5Manisha Dixit6Shoshana Yakar7Mordechai Hallak8Mordechai Hallak9Zvi Laron10Haim Werner11Ilan Bruchim12Ilan Bruchim13Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Tel Aviv, IsraelGynecology Oncology Laboratory, Department of Obstetrics and Gynecology, Hillel Yaffe Medical Center, Hadera, IsraelThe Ruth and Bruce Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, IsraelGynecology Oncology Laboratory, Department of Obstetrics and Gynecology, Hillel Yaffe Medical Center, Hadera, IsraelThe Ruth and Bruce Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, IsraelDepartment of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Tel Aviv, IsraelDavid B. Kriser Dental Center, Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, New York, NY, United StatesDavid B. Kriser Dental Center, Department of Basic Science and Craniofacial Biology, New York University College of Dentistry, New York, NY, United StatesGynecology Oncology Laboratory, Department of Obstetrics and Gynecology, Hillel Yaffe Medical Center, Hadera, IsraelThe Ruth and Bruce Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, IsraelEndocrine and Diabetes Research Unit, Schneider Children’s Medical Center, Petah Tikva, IsraelDepartment of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Tel Aviv, IsraelGynecology Oncology Laboratory, Department of Obstetrics and Gynecology, Hillel Yaffe Medical Center, Hadera, IsraelThe Ruth and Bruce Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Haifa, IsraelThe insulin-like growth factors (IGF) are important players in the development of gynecological malignancies, including epithelial ovarian cancer (EOC). The identification of biomarkers that can help in the diagnosis and scoring of EOC patients is of fundamental importance in clinical oncology. We have recently identified the ZYG11A gene as a new candidate target of IGF1 action. The aim of the present study was to evaluate the expression of ZYG11A in EOC patients and to correlate its pattern of expression with histological grade and pathological stage. Furthermore, and in view of previous analyses showing an interplay between ZYG11A, p53 and the IGF1 receptor (IGF1R), we assessed a potential coordinated expression of these proteins in EOC. In addition, zyg11a expression was assessed in ovaries and uteri of growth hormone receptor (GHR) knock-out mice. Tissue microarray analysis was conducted on 36 patients with EOC and expression of ZYG11A, IGF1R and p53 was assessed by immunohistochemistry. Expression levels were correlated with clinical parameters. qPCR was employed to assess zyg11a mRNA levels in mice tissues. Our analyses provide evidence of reduced ZYG11A expression in high grade tumors, consistent with a putative tumor suppressor role. In addition, an inverse correlation between ZYG11A and p53 levels in individual tumors was noticed. Taken together, our data justify further exploration of the role of ZYG11A as a novel biomarker in EOC.https://www.frontiersin.org/articles/10.3389/fendo.2021.688104/fullZYG11Ainsulin-like growth factor-1 (IGF1)IGF1 receptorp53ovarian cancer |
| spellingShingle | Laris Achlaug Lina Somri-Gannam Lina Somri-Gannam Shilhav Meisel-Sharon Shilhav Meisel-Sharon Rive Sarfstein Manisha Dixit Shoshana Yakar Mordechai Hallak Mordechai Hallak Zvi Laron Haim Werner Ilan Bruchim Ilan Bruchim ZYG11A Is Expressed in Epithelial Ovarian Cancer and Correlates With Low Grade Disease Frontiers in Endocrinology ZYG11A insulin-like growth factor-1 (IGF1) IGF1 receptor p53 ovarian cancer |
| title | ZYG11A Is Expressed in Epithelial Ovarian Cancer and Correlates With Low Grade Disease |
| title_full | ZYG11A Is Expressed in Epithelial Ovarian Cancer and Correlates With Low Grade Disease |
| title_fullStr | ZYG11A Is Expressed in Epithelial Ovarian Cancer and Correlates With Low Grade Disease |
| title_full_unstemmed | ZYG11A Is Expressed in Epithelial Ovarian Cancer and Correlates With Low Grade Disease |
| title_short | ZYG11A Is Expressed in Epithelial Ovarian Cancer and Correlates With Low Grade Disease |
| title_sort | zyg11a is expressed in epithelial ovarian cancer and correlates with low grade disease |
| topic | ZYG11A insulin-like growth factor-1 (IGF1) IGF1 receptor p53 ovarian cancer |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2021.688104/full |
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