The combination of recombinant and non-recombinant Bacillus subtilis spore display technology for presentation of antigen and adjuvant on single spore
Abstract Background Bacillus subtilis spores can be used for presentation of heterologous proteins. Two main approaches have been developed, the recombinant one, requiring modification of bacterial genome to express a protein of interest as a fusion with spore-coat protein, and non-recombinant, base...
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| Format: | Article |
| Language: | English |
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BMC
2017-09-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12934-017-0765-y |
| _version_ | 1819229552161849344 |
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| author | Wojciech Potocki Alessandro Negri Grażyna Peszyńska-Sularz Krzysztof Hinc Michał Obuchowski Adam Iwanicki |
| author_facet | Wojciech Potocki Alessandro Negri Grażyna Peszyńska-Sularz Krzysztof Hinc Michał Obuchowski Adam Iwanicki |
| author_sort | Wojciech Potocki |
| collection | DOAJ |
| description | Abstract Background Bacillus subtilis spores can be used for presentation of heterologous proteins. Two main approaches have been developed, the recombinant one, requiring modification of bacterial genome to express a protein of interest as a fusion with spore-coat protein, and non-recombinant, based on the adsorption of a heterologous protein onto the spore. So far only single proteins have been displayed on the spore surface. Results We have used a combined approach to adsorb and display FliD protein of Clostridium difficile on the surface of recombinant IL-2-presenting spores. Such spores presented FliD protein with efficiency comparable to FliD-adsorbed spores produced by wild-type 168 strain and elicited FliD-specific immune response in intranasally immunized mice. Conclusions Our results indicate that such dual display technology may be useful in creation of spores simultaneously presenting adjuvant and antigen molecules. Regarding the characteristics of elicited immune response it seems plausible that such recombinant IL-2-presenting spores with adsorbed FliD protein might be an interesting candidate for vaccine against infections with Clostridium difficile. |
| first_indexed | 2024-12-23T11:14:59Z |
| format | Article |
| id | doaj.art-1afd39afbf3c4d198991573152f7e916 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-23T11:14:59Z |
| publishDate | 2017-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-1afd39afbf3c4d198991573152f7e9162022-12-21T17:49:14ZengBMCMicrobial Cell Factories1475-28592017-09-0116111110.1186/s12934-017-0765-yThe combination of recombinant and non-recombinant Bacillus subtilis spore display technology for presentation of antigen and adjuvant on single sporeWojciech Potocki0Alessandro Negri1Grażyna Peszyńska-Sularz2Krzysztof Hinc3Michał Obuchowski4Adam Iwanicki5Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, University of GdańskDepartment of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, University of GdańskTri-City Animal Laboratory, Medical University of GdańskDepartment of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of GdańskDepartment of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of GdańskDepartment of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of GdańskAbstract Background Bacillus subtilis spores can be used for presentation of heterologous proteins. Two main approaches have been developed, the recombinant one, requiring modification of bacterial genome to express a protein of interest as a fusion with spore-coat protein, and non-recombinant, based on the adsorption of a heterologous protein onto the spore. So far only single proteins have been displayed on the spore surface. Results We have used a combined approach to adsorb and display FliD protein of Clostridium difficile on the surface of recombinant IL-2-presenting spores. Such spores presented FliD protein with efficiency comparable to FliD-adsorbed spores produced by wild-type 168 strain and elicited FliD-specific immune response in intranasally immunized mice. Conclusions Our results indicate that such dual display technology may be useful in creation of spores simultaneously presenting adjuvant and antigen molecules. Regarding the characteristics of elicited immune response it seems plausible that such recombinant IL-2-presenting spores with adsorbed FliD protein might be an interesting candidate for vaccine against infections with Clostridium difficile.http://link.springer.com/article/10.1186/s12934-017-0765-yBacillus subtilisRecombinant sporesAdsorptionMucosal immunizationFliDClostridium difficile |
| spellingShingle | Wojciech Potocki Alessandro Negri Grażyna Peszyńska-Sularz Krzysztof Hinc Michał Obuchowski Adam Iwanicki The combination of recombinant and non-recombinant Bacillus subtilis spore display technology for presentation of antigen and adjuvant on single spore Microbial Cell Factories Bacillus subtilis Recombinant spores Adsorption Mucosal immunization FliD Clostridium difficile |
| title | The combination of recombinant and non-recombinant Bacillus subtilis spore display technology for presentation of antigen and adjuvant on single spore |
| title_full | The combination of recombinant and non-recombinant Bacillus subtilis spore display technology for presentation of antigen and adjuvant on single spore |
| title_fullStr | The combination of recombinant and non-recombinant Bacillus subtilis spore display technology for presentation of antigen and adjuvant on single spore |
| title_full_unstemmed | The combination of recombinant and non-recombinant Bacillus subtilis spore display technology for presentation of antigen and adjuvant on single spore |
| title_short | The combination of recombinant and non-recombinant Bacillus subtilis spore display technology for presentation of antigen and adjuvant on single spore |
| title_sort | combination of recombinant and non recombinant bacillus subtilis spore display technology for presentation of antigen and adjuvant on single spore |
| topic | Bacillus subtilis Recombinant spores Adsorption Mucosal immunization FliD Clostridium difficile |
| url | http://link.springer.com/article/10.1186/s12934-017-0765-y |
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