Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells
Density-gradient centrifugation is a label-free approach that has been extensively used for cell separations. Though elegant, this process is time-consuming (>30 min), subjects cells to high levels of stress (>350 g) and relies on user skill to enable fractionation of cells that layer...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-08-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/4/3/67 |
| _version_ | 1797705965719519232 |
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| author | Yuxi Sun Palaniappan Sethu |
| author_facet | Yuxi Sun Palaniappan Sethu |
| author_sort | Yuxi Sun |
| collection | DOAJ |
| description | Density-gradient centrifugation is a label-free approach that has been extensively used for cell separations. Though elegant, this process is time-consuming (>30 min), subjects cells to high levels of stress (>350 g) and relies on user skill to enable fractionation of cells that layer as a narrow band between the density-gradient medium and platelet-rich plasma. We hypothesized that microfluidic adaptation of this technique could transform this process into a rapid fractionation approach where samples are separated in a continuous fashion while being exposed to lower levels of stress (<100 g) for shorter durations of time (<3 min). To demonstrate proof-of-concept, we designed a microfluidic density-gradient centrifugation device and constructed a setup to introduce samples and medium like Ficoll in a continuous, pump-less fashion where cells and particles can be exposed to centrifugal force and separated via different outlets. Proof-of-concept studies using binary mixtures of low-density polystyrene beads (1.02 g/cm3) and high-density silicon dioxide beads (2.2 g/cm3) with Ficoll–Paque (1.06 g/cm3) show that separation is indeed feasible with >99% separation efficiency suggesting that this approach can be further adapted for separation of cells. |
| first_indexed | 2024-03-12T05:44:10Z |
| format | Article |
| id | doaj.art-1aff84036a8c412f98057cde0f45e129 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T05:44:10Z |
| publishDate | 2017-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-1aff84036a8c412f98057cde0f45e1292023-09-03T05:41:51ZengMDPI AGBioengineering2306-53542017-08-01436710.3390/bioengineering4030067bioengineering4030067Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and CellsYuxi Sun0Palaniappan Sethu1Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35294, USADivision of Cardiovascular Disease, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USADensity-gradient centrifugation is a label-free approach that has been extensively used for cell separations. Though elegant, this process is time-consuming (>30 min), subjects cells to high levels of stress (>350 g) and relies on user skill to enable fractionation of cells that layer as a narrow band between the density-gradient medium and platelet-rich plasma. We hypothesized that microfluidic adaptation of this technique could transform this process into a rapid fractionation approach where samples are separated in a continuous fashion while being exposed to lower levels of stress (<100 g) for shorter durations of time (<3 min). To demonstrate proof-of-concept, we designed a microfluidic density-gradient centrifugation device and constructed a setup to introduce samples and medium like Ficoll in a continuous, pump-less fashion where cells and particles can be exposed to centrifugal force and separated via different outlets. Proof-of-concept studies using binary mixtures of low-density polystyrene beads (1.02 g/cm3) and high-density silicon dioxide beads (2.2 g/cm3) with Ficoll–Paque (1.06 g/cm3) show that separation is indeed feasible with >99% separation efficiency suggesting that this approach can be further adapted for separation of cells.https://www.mdpi.com/2306-5354/4/3/67cell separationslabel-free cell separationmicrofluidicsdensity-gradient centrifugation |
| spellingShingle | Yuxi Sun Palaniappan Sethu Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells Bioengineering cell separations label-free cell separation microfluidics density-gradient centrifugation |
| title | Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells |
| title_full | Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells |
| title_fullStr | Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells |
| title_full_unstemmed | Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells |
| title_short | Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells |
| title_sort | microfluidic adaptation of density gradient centrifugation for isolation of particles and cells |
| topic | cell separations label-free cell separation microfluidics density-gradient centrifugation |
| url | https://www.mdpi.com/2306-5354/4/3/67 |
| work_keys_str_mv | AT yuxisun microfluidicadaptationofdensitygradientcentrifugationforisolationofparticlesandcells AT palaniappansethu microfluidicadaptationofdensitygradientcentrifugationforisolationofparticlesandcells |