Synthesis of Carboxylated Magnetite Nanoparticles Covalent Conjugates with Folic Acid Antibody FA-1 for Lateral Flow Immunoassay
Magnetite nanoparticles (MNPs) are a preferable material for different bioassays because of their quite low toxicity both for cells and for mammals, and they have a big variety of surface functionalization approaches. We have synthesized MNPs via a simple and convenient co-precipitation method with...
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MDPI AG
2023-10-01
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| Series: | Engineering Proceedings |
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| Online Access: | https://www.mdpi.com/2673-4591/48/1/66 |
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| author | Maria O. Zolotova Sergey L. Znoyko Alexey V. Orlov Petr I. Nikitin Artem V. Sinolits |
| author_facet | Maria O. Zolotova Sergey L. Znoyko Alexey V. Orlov Petr I. Nikitin Artem V. Sinolits |
| author_sort | Maria O. Zolotova |
| collection | DOAJ |
| description | Magnetite nanoparticles (MNPs) are a preferable material for different bioassays because of their quite low toxicity both for cells and for mammals, and they have a big variety of surface functionalization approaches. We have synthesized MNPs via a simple and convenient co-precipitation method with preliminary filtration of FeCl<sub>2</sub> and FeCl<sub>3</sub> solution, under argon atmosphere and non-magnetic stirring. MNPs were citrate-stabilized and then modified stage by stage with tetraethoxysilane (TEOS), (3-Aminopropyl)triethoxysilane (APTES) and acylated with succinic anhydride, resulting in carboxylated MNPs. Carboxylated MNPs were covalently bounded with folic acid antibody (FA-1) via 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). MNP-EDC-FA-1 were passed through a test-stripe with the line consisting of folic acid–gelatin conjugate. The conjugation of MNP-EDC-FA-1 with folic acid was observed visually, and the magnetic signal distribution was scanned through the test-stripe with the magnetic particle quantification technique (MPQ) developed earlier. Visually, the line with folic acid–gelatin conjugate on the test-stripe turned dark, with color intensity strongly depending on the MNP-EDC-FA-1 concentration. MPQ has shown that the great majority of MNP-EDC-FA-1 was bound with the acid–gelatin conjugate. The MPQ technique allowed quantification down to 5 ng of MNP-EDC-FA-1 in this experiment with MNPs synthesized, with a strong peak at the acid–conjugate line. |
| first_indexed | 2024-04-24T18:19:53Z |
| format | Article |
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| issn | 2673-4591 |
| language | English |
| last_indexed | 2024-04-24T18:19:53Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Engineering Proceedings |
| spelling | doaj.art-1b0041b3e7ba475cb1bfbf660f34f89d2024-03-27T13:36:12ZengMDPI AGEngineering Proceedings2673-45912023-10-014816610.3390/CSAC2023-15169Synthesis of Carboxylated Magnetite Nanoparticles Covalent Conjugates with Folic Acid Antibody FA-1 for Lateral Flow ImmunoassayMaria O. Zolotova0Sergey L. Znoyko1Alexey V. Orlov2Petr I. Nikitin3Artem V. Sinolits4Prokhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov Street, 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov Street, 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov Street, 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov Street, 119991 Moscow, RussiaProkhorov General Physics Institute of the Russian Academy of Sciences, 38 Vavilov Street, 119991 Moscow, RussiaMagnetite nanoparticles (MNPs) are a preferable material for different bioassays because of their quite low toxicity both for cells and for mammals, and they have a big variety of surface functionalization approaches. We have synthesized MNPs via a simple and convenient co-precipitation method with preliminary filtration of FeCl<sub>2</sub> and FeCl<sub>3</sub> solution, under argon atmosphere and non-magnetic stirring. MNPs were citrate-stabilized and then modified stage by stage with tetraethoxysilane (TEOS), (3-Aminopropyl)triethoxysilane (APTES) and acylated with succinic anhydride, resulting in carboxylated MNPs. Carboxylated MNPs were covalently bounded with folic acid antibody (FA-1) via 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). MNP-EDC-FA-1 were passed through a test-stripe with the line consisting of folic acid–gelatin conjugate. The conjugation of MNP-EDC-FA-1 with folic acid was observed visually, and the magnetic signal distribution was scanned through the test-stripe with the magnetic particle quantification technique (MPQ) developed earlier. Visually, the line with folic acid–gelatin conjugate on the test-stripe turned dark, with color intensity strongly depending on the MNP-EDC-FA-1 concentration. MPQ has shown that the great majority of MNP-EDC-FA-1 was bound with the acid–gelatin conjugate. The MPQ technique allowed quantification down to 5 ng of MNP-EDC-FA-1 in this experiment with MNPs synthesized, with a strong peak at the acid–conjugate line.https://www.mdpi.com/2673-4591/48/1/66Magnetite nanoparticlesfolic acidmagnetic chemosensorsantibody conjugationlateral flow assay |
| spellingShingle | Maria O. Zolotova Sergey L. Znoyko Alexey V. Orlov Petr I. Nikitin Artem V. Sinolits Synthesis of Carboxylated Magnetite Nanoparticles Covalent Conjugates with Folic Acid Antibody FA-1 for Lateral Flow Immunoassay Engineering Proceedings Magnetite nanoparticles folic acid magnetic chemosensors antibody conjugation lateral flow assay |
| title | Synthesis of Carboxylated Magnetite Nanoparticles Covalent Conjugates with Folic Acid Antibody FA-1 for Lateral Flow Immunoassay |
| title_full | Synthesis of Carboxylated Magnetite Nanoparticles Covalent Conjugates with Folic Acid Antibody FA-1 for Lateral Flow Immunoassay |
| title_fullStr | Synthesis of Carboxylated Magnetite Nanoparticles Covalent Conjugates with Folic Acid Antibody FA-1 for Lateral Flow Immunoassay |
| title_full_unstemmed | Synthesis of Carboxylated Magnetite Nanoparticles Covalent Conjugates with Folic Acid Antibody FA-1 for Lateral Flow Immunoassay |
| title_short | Synthesis of Carboxylated Magnetite Nanoparticles Covalent Conjugates with Folic Acid Antibody FA-1 for Lateral Flow Immunoassay |
| title_sort | synthesis of carboxylated magnetite nanoparticles covalent conjugates with folic acid antibody fa 1 for lateral flow immunoassay |
| topic | Magnetite nanoparticles folic acid magnetic chemosensors antibody conjugation lateral flow assay |
| url | https://www.mdpi.com/2673-4591/48/1/66 |
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