Antibodies for the Coat Protein of Cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease
The cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein w...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.814119/full |
| _version_ | 1811292275214385152 |
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| author | Lucia Vieira Hoffmann Amanda Alves Branquinho Paulo Augusto Vianna Barroso Maite F. S. Vaslin |
| author_facet | Lucia Vieira Hoffmann Amanda Alves Branquinho Paulo Augusto Vianna Barroso Maite F. S. Vaslin |
| author_sort | Lucia Vieira Hoffmann |
| collection | DOAJ |
| description | The cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (α-GQE, α-PRN, and α-INK) and monoclonal (α-GQE, α-PRN, and α-NKF) antibodies. All six were tested as capture antibodies, and polyclonal α-GQE and the monocle onal α-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures. |
| first_indexed | 2024-04-13T04:42:58Z |
| format | Article |
| id | doaj.art-1b014ad5242844c28c6b188b1cfec1cc |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T04:42:58Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1b014ad5242844c28c6b188b1cfec1cc2022-12-22T03:01:56ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-07-011310.3389/fpls.2022.814119814119Antibodies for the Coat Protein of Cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue DiseaseLucia Vieira Hoffmann0Amanda Alves Branquinho1Paulo Augusto Vianna Barroso2Maite F. S. Vaslin3Brazilian Agricultural Research Corporation (EMBRAPA), Brasília, BrazilBiological Science Institute, Universidade Federal de Goiás, Goiânia, BrazilBrazilian Agricultural Research Corporation (EMBRAPA), Brasília, BrazilVirology Department, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilThe cotton blue disease, caused by the cotton leafroll dwarf virus (CLRDV), leads to dwarfism, leaf rolling, and production loss in susceptible cotton varieties. To develop an enzyme-linked immunosorbent assay (ELISA) test to detect the virus in cotton and weeds, peptides based on the coat protein were used to produce polyclonal (α-GQE, α-PRN, and α-INK) and monoclonal (α-GQE, α-PRN, and α-NKF) antibodies. All six were tested as capture antibodies, and polyclonal α-GQE and the monocle onal α-NKF were labeled with the enzyme alkaline phosphatase and used as detection antibodies for a double antibody sandwich (DAS) ELISA method, in which p-nitrophenyl phosphate was added and measured by absorbance at 405 nm. The DAS-ELISA sandwich was efficient in discriminating between healthy and diseased plant extracts. The ELISA methodology detected the virus in the weeds Commelina sp., which was confirmed by RT-PCR. The monoclonal antibodies may be used to develop other diagnostic procedures.https://www.frontiersin.org/articles/10.3389/fpls.2022.814119/fullenzyme-linked immunosorbent assay (ELISA)CLRDVvirusintermediary hostserology |
| spellingShingle | Lucia Vieira Hoffmann Amanda Alves Branquinho Paulo Augusto Vianna Barroso Maite F. S. Vaslin Antibodies for the Coat Protein of Cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease Frontiers in Plant Science enzyme-linked immunosorbent assay (ELISA) CLRDV virus intermediary host serology |
| title | Antibodies for the Coat Protein of Cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease |
| title_full | Antibodies for the Coat Protein of Cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease |
| title_fullStr | Antibodies for the Coat Protein of Cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease |
| title_full_unstemmed | Antibodies for the Coat Protein of Cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease |
| title_short | Antibodies for the Coat Protein of Cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease |
| title_sort | antibodies for the coat protein of cotton leafroll dwarf virus detect commelina sp as an intermediary host for cotton blue disease |
| topic | enzyme-linked immunosorbent assay (ELISA) CLRDV virus intermediary host serology |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.814119/full |
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