Сolonial–morphological characteristics of dissociatives of the Rhodococcus ruber - 8/4/1 strain
This article provides a study of the molecular, physiological, biochemical and morphological features of R-, S- and M-dissociants of Rhodococcus ruber - 8/4/1. The sequence similarity of the genome fragments of R- and S-dissociant forms encoded by 16S rRNA was demonstrated. MALDI spectra were obtai...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2023-10-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | https://ojs.uem.br/ojs/index.php/ActaSciBiolSci/article/view/65392 |
| _version_ | 1797648005458821120 |
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| author | Barno Alimova Ozodakhon Pulatova Akhmadjon Makhsumkhanov Lobar Khasanova Kakhramon Davranov |
| author_facet | Barno Alimova Ozodakhon Pulatova Akhmadjon Makhsumkhanov Lobar Khasanova Kakhramon Davranov |
| author_sort | Barno Alimova |
| collection | DOAJ |
| description |
This article provides a study of the molecular, physiological, biochemical and morphological features of R-, S- and M-dissociants of Rhodococcus ruber - 8/4/1. The sequence similarity of the genome fragments of R- and S-dissociant forms encoded by 16S rRNA was demonstrated. MALDI spectra were obtained for the R. ruber-8/4/1 strain and its dissociants. Under standard analysis conditions, the MALDI profiles of R-, S- and M- forms dissociants of the Rhodococcus ruber - 8/4/1 were similar. The determination of the resistance of the strain dissociants to external factors showed that R-forms were more stable under UV irradiation and increased temperature. The S-forms showed stability upon the conversion of acrylic acid nitrile into acrylamide (AA), whereas the M-forms were stable under a decrease in the cultivation temperature, and an increase in the concentration of NaCl in the medium. The S-forms proved to be the most active in the transformation of acrylonitrile into AA.
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| first_indexed | 2024-03-11T15:24:53Z |
| format | Article |
| id | doaj.art-1b04f799f52541ca9b1458117b01b4d8 |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-03-11T15:24:53Z |
| publishDate | 2023-10-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-1b04f799f52541ca9b1458117b01b4d82023-10-27T17:59:30ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2023-10-0145110.4025/actascibiolsci.v45i1.65392Сolonial–morphological characteristics of dissociatives of the Rhodococcus ruber - 8/4/1 strainBarno Alimova0Ozodakhon Pulatova1Akhmadjon Makhsumkhanov2Lobar Khasanova 3Kakhramon Davranov 4Academy of Sciences of the Republic of UzbekistanAcademy of Sciences of the Republic of UzbekistanAcademy of Sciences of the Republic of UzbekistanAcademy of Sciences of the Republic of UzbekistanAcademy of Sciences of the Republic of Uzbekistan This article provides a study of the molecular, physiological, biochemical and morphological features of R-, S- and M-dissociants of Rhodococcus ruber - 8/4/1. The sequence similarity of the genome fragments of R- and S-dissociant forms encoded by 16S rRNA was demonstrated. MALDI spectra were obtained for the R. ruber-8/4/1 strain and its dissociants. Under standard analysis conditions, the MALDI profiles of R-, S- and M- forms dissociants of the Rhodococcus ruber - 8/4/1 were similar. The determination of the resistance of the strain dissociants to external factors showed that R-forms were more stable under UV irradiation and increased temperature. The S-forms showed stability upon the conversion of acrylic acid nitrile into acrylamide (AA), whereas the M-forms were stable under a decrease in the cultivation temperature, and an increase in the concentration of NaCl in the medium. The S-forms proved to be the most active in the transformation of acrylonitrile into AA. https://ojs.uem.br/ojs/index.php/ActaSciBiolSci/article/view/65392dissociation; forms; strain; Rhodococcus ruber - 8/4/1; biotransformation; nitrile hydratase. |
| spellingShingle | Barno Alimova Ozodakhon Pulatova Akhmadjon Makhsumkhanov Lobar Khasanova Kakhramon Davranov Сolonial–morphological characteristics of dissociatives of the Rhodococcus ruber - 8/4/1 strain Acta Scientiarum: Biological Sciences dissociation; forms; strain; Rhodococcus ruber - 8/4/1; biotransformation; nitrile hydratase. |
| title | Сolonial–morphological characteristics of dissociatives of the Rhodococcus ruber - 8/4/1 strain |
| title_full | Сolonial–morphological characteristics of dissociatives of the Rhodococcus ruber - 8/4/1 strain |
| title_fullStr | Сolonial–morphological characteristics of dissociatives of the Rhodococcus ruber - 8/4/1 strain |
| title_full_unstemmed | Сolonial–morphological characteristics of dissociatives of the Rhodococcus ruber - 8/4/1 strain |
| title_short | Сolonial–morphological characteristics of dissociatives of the Rhodococcus ruber - 8/4/1 strain |
| title_sort | сolonial morphological characteristics of dissociatives of the rhodococcus ruber 8 4 1 strain |
| topic | dissociation; forms; strain; Rhodococcus ruber - 8/4/1; biotransformation; nitrile hydratase. |
| url | https://ojs.uem.br/ojs/index.php/ActaSciBiolSci/article/view/65392 |
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