Multiple Genes Related to Muscle Identified through a Joint Analysis of a Two-stage Genome-wide Association Study for Racing Performance of 1,156 Thoroughbreds
Thoroughbred, a relatively recent horse breed, is best known for its use in horse racing. Although myostatin (MSTN) variants have been reported to be highly associated with horse racing performance, the trait is more likely to be polygenic in nature. The purpose of this study was to identify genetic...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2015-06-01
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| Series: | Asian-Australasian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | http://www.ajas.info/upload/pdf/ajas-28-6-771.pdf |
| _version_ | 1818201402550255616 |
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| author | Dong-Hyun Shin Jin Woo Lee Jong-Eun Park Ik-Young Choi Hee-Seok Oh Hyeon Jeong Kim Heebal Kim |
| author_facet | Dong-Hyun Shin Jin Woo Lee Jong-Eun Park Ik-Young Choi Hee-Seok Oh Hyeon Jeong Kim Heebal Kim |
| author_sort | Dong-Hyun Shin |
| collection | DOAJ |
| description | Thoroughbred, a relatively recent horse breed, is best known for its use in horse racing. Although myostatin (MSTN) variants have been reported to be highly associated with horse racing performance, the trait is more likely to be polygenic in nature. The purpose of this study was to identify genetic variants strongly associated with racing performance by using estimated breeding value (EBV) for race time as a phenotype. We conducted a two-stage genome-wide association study to search for genetic variants associated with the EBV. In the first stage of genome-wide association study, a relatively large number of markers (~54,000 single-nucleotide polymorphisms, SNPs) were evaluated in a small number of samples (240 horses). In the second stage, a relatively small number of markers identified to have large effects (170 SNPs) were evaluated in a much larger number of samples (1,156 horses). We also validated the SNPs related to MSTN known to have large effects on racing performance and found significant associations in the stage two analysis, but not in stage one. We identified 28 significant SNPs related to 17 genes. Among these, six genes have a function related to myogenesis and five genes are involved in muscle maintenance. To our knowledge, these genes are newly reported for the genetic association with racing performance of Thoroughbreds. It complements a recent horse genome-wide association studies of racing performance that identified other SNPs and genes as the most significant variants. These results will help to expand our knowledge of the polygenic nature of racing performance in Thoroughbreds. |
| first_indexed | 2024-12-12T02:52:59Z |
| format | Article |
| id | doaj.art-1b05c2a54867436fae0b533c660e7d2b |
| institution | Directory Open Access Journal |
| issn | 1011-2367 1976-5517 |
| language | English |
| last_indexed | 2024-12-12T02:52:59Z |
| publishDate | 2015-06-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Asian-Australasian Journal of Animal Sciences |
| spelling | doaj.art-1b05c2a54867436fae0b533c660e7d2b2022-12-22T00:40:50ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172015-06-0128677178110.5713/ajas.14.000823081Multiple Genes Related to Muscle Identified through a Joint Analysis of a Two-stage Genome-wide Association Study for Racing Performance of 1,156 ThoroughbredsDong-Hyun Shin0Jin Woo Lee1Jong-Eun Park2Ik-Young Choi3Hee-Seok Oh4Hyeon Jeong Kim5Heebal Kim6 Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea Horse Industry Research Center, Korea Racing Authority (KRA), Gwacheon 427-711, .Korea Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea Genome Analysis Center, National Instrumentation and Environmental Management (NICEM), Seoul National University, Seoul 151-921, .Korea Department of Statistics, Seoul National University, Seoul 151-747, .Korea C&K Genomics, Seoul 151-742, .Korea Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, KoreaThoroughbred, a relatively recent horse breed, is best known for its use in horse racing. Although myostatin (MSTN) variants have been reported to be highly associated with horse racing performance, the trait is more likely to be polygenic in nature. The purpose of this study was to identify genetic variants strongly associated with racing performance by using estimated breeding value (EBV) for race time as a phenotype. We conducted a two-stage genome-wide association study to search for genetic variants associated with the EBV. In the first stage of genome-wide association study, a relatively large number of markers (~54,000 single-nucleotide polymorphisms, SNPs) were evaluated in a small number of samples (240 horses). In the second stage, a relatively small number of markers identified to have large effects (170 SNPs) were evaluated in a much larger number of samples (1,156 horses). We also validated the SNPs related to MSTN known to have large effects on racing performance and found significant associations in the stage two analysis, but not in stage one. We identified 28 significant SNPs related to 17 genes. Among these, six genes have a function related to myogenesis and five genes are involved in muscle maintenance. To our knowledge, these genes are newly reported for the genetic association with racing performance of Thoroughbreds. It complements a recent horse genome-wide association studies of racing performance that identified other SNPs and genes as the most significant variants. These results will help to expand our knowledge of the polygenic nature of racing performance in Thoroughbreds.http://www.ajas.info/upload/pdf/ajas-28-6-771.pdfGenome-wide Association Studies [GWAS]ThoroughbredRacing PerformanceSingle Nucleotide PolymorphismEstimated Breeding Value |
| spellingShingle | Dong-Hyun Shin Jin Woo Lee Jong-Eun Park Ik-Young Choi Hee-Seok Oh Hyeon Jeong Kim Heebal Kim Multiple Genes Related to Muscle Identified through a Joint Analysis of a Two-stage Genome-wide Association Study for Racing Performance of 1,156 Thoroughbreds Asian-Australasian Journal of Animal Sciences Genome-wide Association Studies [GWAS] Thoroughbred Racing Performance Single Nucleotide Polymorphism Estimated Breeding Value |
| title | Multiple Genes Related to Muscle Identified through a Joint Analysis of a Two-stage Genome-wide Association Study for Racing Performance of 1,156 Thoroughbreds |
| title_full | Multiple Genes Related to Muscle Identified through a Joint Analysis of a Two-stage Genome-wide Association Study for Racing Performance of 1,156 Thoroughbreds |
| title_fullStr | Multiple Genes Related to Muscle Identified through a Joint Analysis of a Two-stage Genome-wide Association Study for Racing Performance of 1,156 Thoroughbreds |
| title_full_unstemmed | Multiple Genes Related to Muscle Identified through a Joint Analysis of a Two-stage Genome-wide Association Study for Racing Performance of 1,156 Thoroughbreds |
| title_short | Multiple Genes Related to Muscle Identified through a Joint Analysis of a Two-stage Genome-wide Association Study for Racing Performance of 1,156 Thoroughbreds |
| title_sort | multiple genes related to muscle identified through a joint analysis of a two stage genome wide association study for racing performance of 1 156 thoroughbreds |
| topic | Genome-wide Association Studies [GWAS] Thoroughbred Racing Performance Single Nucleotide Polymorphism Estimated Breeding Value |
| url | http://www.ajas.info/upload/pdf/ajas-28-6-771.pdf |
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