Molecular constraints on tolerance‐resistance trade‐offs: Is there a cost?
Abstract Plants possess myriad defenses against their herbivores, including constitutive and inducible chemical compounds and regrowth strategies known as tolerance. Recent studies have shown that plant tolerance and resistance are positively associated given they are co‐localized in the same molecu...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-12-01
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| Series: | Plant-Environment Interactions |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pei3.10125 |
| _version_ | 1797397256272347136 |
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| author | J. Miles Mesa Ken N. Paige |
| author_facet | J. Miles Mesa Ken N. Paige |
| author_sort | J. Miles Mesa |
| collection | DOAJ |
| description | Abstract Plants possess myriad defenses against their herbivores, including constitutive and inducible chemical compounds and regrowth strategies known as tolerance. Recent studies have shown that plant tolerance and resistance are positively associated given they are co‐localized in the same molecular pathway, the oxidative pentose phosphate pathway. However, given that both defensive strategies utilize carbon skeletons from a shared resource pool in the oxidative pentose phosphate pathway there are likely costs in maintaining both resistance‐tolerance strategies. Here we investigate fitness costs in maintaining both strategies by utilizing a double knockout of cyp79B2 and cyp79B3, key enzymes in the biosynthetic process of indole glucosinolates, which convert tryptophan to indole‐3‐acetaldoxime (IAOx) and is further used to produce indole glucosinolates. These mutant plants are devoid of any indole glucosinolates thus reducing plant resistance. Results show that knocking out indole glucosinolate production and thus one of the resistance pathways leads to an approximate 94% increase in fitness compensation shifting the undercompensating wild‐type Columbia‐0 to an overcompensating genotype following damage. We discuss the potential mechanistic basis for the observed patterns. |
| first_indexed | 2024-03-09T01:08:19Z |
| format | Article |
| id | doaj.art-1b07d0d9eb6643848a370a369e8c3b02 |
| institution | Directory Open Access Journal |
| issn | 2575-6265 |
| language | English |
| last_indexed | 2024-03-09T01:08:19Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant-Environment Interactions |
| spelling | doaj.art-1b07d0d9eb6643848a370a369e8c3b022023-12-11T09:10:53ZengWileyPlant-Environment Interactions2575-62652023-12-014631732310.1002/pei3.10125Molecular constraints on tolerance‐resistance trade‐offs: Is there a cost?J. Miles Mesa0Ken N. Paige1Department of Evolution, Ecology and Behavior University of Illinois at Urbana–Champaign Urbana Illinois USADepartment of Evolution, Ecology and Behavior University of Illinois at Urbana–Champaign Urbana Illinois USAAbstract Plants possess myriad defenses against their herbivores, including constitutive and inducible chemical compounds and regrowth strategies known as tolerance. Recent studies have shown that plant tolerance and resistance are positively associated given they are co‐localized in the same molecular pathway, the oxidative pentose phosphate pathway. However, given that both defensive strategies utilize carbon skeletons from a shared resource pool in the oxidative pentose phosphate pathway there are likely costs in maintaining both resistance‐tolerance strategies. Here we investigate fitness costs in maintaining both strategies by utilizing a double knockout of cyp79B2 and cyp79B3, key enzymes in the biosynthetic process of indole glucosinolates, which convert tryptophan to indole‐3‐acetaldoxime (IAOx) and is further used to produce indole glucosinolates. These mutant plants are devoid of any indole glucosinolates thus reducing plant resistance. Results show that knocking out indole glucosinolate production and thus one of the resistance pathways leads to an approximate 94% increase in fitness compensation shifting the undercompensating wild‐type Columbia‐0 to an overcompensating genotype following damage. We discuss the potential mechanistic basis for the observed patterns.https://doi.org/10.1002/pei3.10125Arabidopsisendoreduplicationglucosinolatesovercompensationoxidative pentose phosphate pathwayresistance‐tolerance tradeoffs |
| spellingShingle | J. Miles Mesa Ken N. Paige Molecular constraints on tolerance‐resistance trade‐offs: Is there a cost? Plant-Environment Interactions Arabidopsis endoreduplication glucosinolates overcompensation oxidative pentose phosphate pathway resistance‐tolerance tradeoffs |
| title | Molecular constraints on tolerance‐resistance trade‐offs: Is there a cost? |
| title_full | Molecular constraints on tolerance‐resistance trade‐offs: Is there a cost? |
| title_fullStr | Molecular constraints on tolerance‐resistance trade‐offs: Is there a cost? |
| title_full_unstemmed | Molecular constraints on tolerance‐resistance trade‐offs: Is there a cost? |
| title_short | Molecular constraints on tolerance‐resistance trade‐offs: Is there a cost? |
| title_sort | molecular constraints on tolerance resistance trade offs is there a cost |
| topic | Arabidopsis endoreduplication glucosinolates overcompensation oxidative pentose phosphate pathway resistance‐tolerance tradeoffs |
| url | https://doi.org/10.1002/pei3.10125 |
| work_keys_str_mv | AT jmilesmesa molecularconstraintsontoleranceresistancetradeoffsisthereacost AT kennpaige molecularconstraintsontoleranceresistancetradeoffsisthereacost |