Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples
Enzymatic luminescent systems are a promising tool for rapid detection of heavy metals ions for water quality assessment. Nevertheless, their widespread use is limited by the lack of test procedure automation and available sensitive handheld luminometers. Herein we describe integration of disposable...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-03-01
|
| Series: | Chemosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9040/7/1/16 |
| _version_ | 1819141968583720960 |
|---|---|
| author | Kirill A. Lukyanenko Ivan A. Denisov Vladimir V. Sorokin Anton S. Yakimov Elena N. Esimbekova Peter I. Belobrov |
| author_facet | Kirill A. Lukyanenko Ivan A. Denisov Vladimir V. Sorokin Anton S. Yakimov Elena N. Esimbekova Peter I. Belobrov |
| author_sort | Kirill A. Lukyanenko |
| collection | DOAJ |
| description | Enzymatic luminescent systems are a promising tool for rapid detection of heavy metals ions for water quality assessment. Nevertheless, their widespread use is limited by the lack of test procedure automation and available sensitive handheld luminometers. Herein we describe integration of disposable microfluidic chips for bioluminescent enzyme-inhibition based assay with a handheld luminometer, which detection system is based on a thermally stabilized silicon photomultiplier (SiPM). Microfluidic chips were made of poly(methyl methacrylate) by micro-milling method and sealed using a solvent bonding technique. The composition of the bioluminescent system in microfluidic chip was optimized to achieve higher luminescence intensity and storage time. Results indicate that developed device provided comparable sensitivity with bench-scale PMT-based commercial luminometers. Limit of detection for copper (II) sulfate reached 2.5 mg/L for developed biosensor. Hereby we proved the concept of handheld enzymatic optical biosensors with disposable chips for bioassay. The proposed biosensor can be used as an early warning field-deployable system for rapid detection of heavy metals salts and other toxic chemicals, which affect bioluminescent signal of enzymatic reaction. |
| first_indexed | 2024-12-22T12:02:53Z |
| format | Article |
| id | doaj.art-1b08ed6c4740415e8ea9d033875b35f0 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-12-22T12:02:53Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-1b08ed6c4740415e8ea9d033875b35f02022-12-21T18:26:30ZengMDPI AGChemosensors2227-90402019-03-01711610.3390/chemosensors7010016chemosensors7010016Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water SamplesKirill A. Lukyanenko0Ivan A. Denisov1Vladimir V. Sorokin2Anton S. Yakimov3Elena N. Esimbekova4Peter I. Belobrov5Laboratory for Digital Controlled Drugs and Theranostics, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk 660036, RussiaLaboratory for Digital Controlled Drugs and Theranostics, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk 660036, RussiaLaboratory of Bioluminescent Biotechnologies, Siberian Federal University, Krasnoyarsk 660041, RussiaResearch Institute of Molecular Medicine and Pathobiochemistry, Krasnoyarsk State Medical University named after Prof. V.F. Voino-Yasenetsky, Krasnoyarsk 660022, RussiaLaboratory of Bioluminescent Biotechnologies, Siberian Federal University, Krasnoyarsk 660041, RussiaLaboratory of Bioluminescent Biotechnologies, Siberian Federal University, Krasnoyarsk 660041, RussiaEnzymatic luminescent systems are a promising tool for rapid detection of heavy metals ions for water quality assessment. Nevertheless, their widespread use is limited by the lack of test procedure automation and available sensitive handheld luminometers. Herein we describe integration of disposable microfluidic chips for bioluminescent enzyme-inhibition based assay with a handheld luminometer, which detection system is based on a thermally stabilized silicon photomultiplier (SiPM). Microfluidic chips were made of poly(methyl methacrylate) by micro-milling method and sealed using a solvent bonding technique. The composition of the bioluminescent system in microfluidic chip was optimized to achieve higher luminescence intensity and storage time. Results indicate that developed device provided comparable sensitivity with bench-scale PMT-based commercial luminometers. Limit of detection for copper (II) sulfate reached 2.5 mg/L for developed biosensor. Hereby we proved the concept of handheld enzymatic optical biosensors with disposable chips for bioassay. The proposed biosensor can be used as an early warning field-deployable system for rapid detection of heavy metals salts and other toxic chemicals, which affect bioluminescent signal of enzymatic reaction.https://www.mdpi.com/2227-9040/7/1/16chemical measurementssilicon photomultiplieroptical biosensorbioassaymicrofluidicsluciferasebioluminescence |
| spellingShingle | Kirill A. Lukyanenko Ivan A. Denisov Vladimir V. Sorokin Anton S. Yakimov Elena N. Esimbekova Peter I. Belobrov Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples Chemosensors chemical measurements silicon photomultiplier optical biosensor bioassay microfluidics luciferase bioluminescence |
| title | Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples |
| title_full | Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples |
| title_fullStr | Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples |
| title_full_unstemmed | Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples |
| title_short | Handheld Enzymatic Luminescent Biosensor for Rapid Detection of Heavy Metals in Water Samples |
| title_sort | handheld enzymatic luminescent biosensor for rapid detection of heavy metals in water samples |
| topic | chemical measurements silicon photomultiplier optical biosensor bioassay microfluidics luciferase bioluminescence |
| url | https://www.mdpi.com/2227-9040/7/1/16 |
| work_keys_str_mv | AT kirillalukyanenko handheldenzymaticluminescentbiosensorforrapiddetectionofheavymetalsinwatersamples AT ivanadenisov handheldenzymaticluminescentbiosensorforrapiddetectionofheavymetalsinwatersamples AT vladimirvsorokin handheldenzymaticluminescentbiosensorforrapiddetectionofheavymetalsinwatersamples AT antonsyakimov handheldenzymaticluminescentbiosensorforrapiddetectionofheavymetalsinwatersamples AT elenanesimbekova handheldenzymaticluminescentbiosensorforrapiddetectionofheavymetalsinwatersamples AT peteribelobrov handheldenzymaticluminescentbiosensorforrapiddetectionofheavymetalsinwatersamples |