Mean Centered Kinetic—Spectrophotometric Data—Continuous Wavelet Transform for Simultaneous Determination of Dopamine and Uric Acid in Presence of Ascorbic Acid at Biological Samples
In the present study, a rapid, practical, selective and sensitive method was proposed for simultaneous determination of dopamine (DA) and uric acid (UA). Kinetic profiles of analytes were recorded and transformed by different mother wavelets. The contribution of ascorbic acid was neglected using mea...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Separations |
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| Online Access: | https://www.mdpi.com/2297-8739/9/10/282 |
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| author | Maryam Abbasi Tarighat Zahara Keshavarz Gholamreza Abdi Charalampos Proestos |
| author_facet | Maryam Abbasi Tarighat Zahara Keshavarz Gholamreza Abdi Charalampos Proestos |
| author_sort | Maryam Abbasi Tarighat |
| collection | DOAJ |
| description | In the present study, a rapid, practical, selective and sensitive method was proposed for simultaneous determination of dopamine (DA) and uric acid (UA). Kinetic profiles of analytes were recorded and transformed by different mother wavelets. The contribution of ascorbic acid was neglected using mean centering of data before transformation. The concentrations range were 1.0–60.0 μmol L<sup>−1</sup> and 7–40.0 μmol L<sup>−1</sup>, with detection limit of 0.06 and 0.30 μmol L<sup>−1</sup> for uric acid and dopamine, respectively. The results of sym8 and db4 mother wavelets showed that proposed method creates a most selective and sensitive determination without using initial separation steps. The obtained results by CWT-sym8 and CWT-db4 were compared with partial least squares (PLS) results. The simultaneous quantitation of DA and UA with the proposed method was successfully applied in different urine and serum samples. |
| first_indexed | 2024-03-09T19:29:26Z |
| format | Article |
| id | doaj.art-1b0b51c9f4c046fd82f9da389ba2bc0b |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-09T19:29:26Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
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| series | Separations |
| spelling | doaj.art-1b0b51c9f4c046fd82f9da389ba2bc0b2023-11-24T02:32:06ZengMDPI AGSeparations2297-87392022-10-0191028210.3390/separations9100282Mean Centered Kinetic—Spectrophotometric Data—Continuous Wavelet Transform for Simultaneous Determination of Dopamine and Uric Acid in Presence of Ascorbic Acid at Biological SamplesMaryam Abbasi Tarighat0Zahara Keshavarz1Gholamreza Abdi2Charalampos Proestos3Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr 75169, IranFaculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr 75169, IranDepartment of Biotechnology, Persian Gulf Research Institute, Persian Gulf University, Bushehr 7516, IranFood Chemistry Laboratory, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, GreeceIn the present study, a rapid, practical, selective and sensitive method was proposed for simultaneous determination of dopamine (DA) and uric acid (UA). Kinetic profiles of analytes were recorded and transformed by different mother wavelets. The contribution of ascorbic acid was neglected using mean centering of data before transformation. The concentrations range were 1.0–60.0 μmol L<sup>−1</sup> and 7–40.0 μmol L<sup>−1</sup>, with detection limit of 0.06 and 0.30 μmol L<sup>−1</sup> for uric acid and dopamine, respectively. The results of sym8 and db4 mother wavelets showed that proposed method creates a most selective and sensitive determination without using initial separation steps. The obtained results by CWT-sym8 and CWT-db4 were compared with partial least squares (PLS) results. The simultaneous quantitation of DA and UA with the proposed method was successfully applied in different urine and serum samples.https://www.mdpi.com/2297-8739/9/10/282kineticsimultaneous determinationuric aciddopamineCWT |
| spellingShingle | Maryam Abbasi Tarighat Zahara Keshavarz Gholamreza Abdi Charalampos Proestos Mean Centered Kinetic—Spectrophotometric Data—Continuous Wavelet Transform for Simultaneous Determination of Dopamine and Uric Acid in Presence of Ascorbic Acid at Biological Samples Separations kinetic simultaneous determination uric acid dopamine CWT |
| title | Mean Centered Kinetic—Spectrophotometric Data—Continuous Wavelet Transform for Simultaneous Determination of Dopamine and Uric Acid in Presence of Ascorbic Acid at Biological Samples |
| title_full | Mean Centered Kinetic—Spectrophotometric Data—Continuous Wavelet Transform for Simultaneous Determination of Dopamine and Uric Acid in Presence of Ascorbic Acid at Biological Samples |
| title_fullStr | Mean Centered Kinetic—Spectrophotometric Data—Continuous Wavelet Transform for Simultaneous Determination of Dopamine and Uric Acid in Presence of Ascorbic Acid at Biological Samples |
| title_full_unstemmed | Mean Centered Kinetic—Spectrophotometric Data—Continuous Wavelet Transform for Simultaneous Determination of Dopamine and Uric Acid in Presence of Ascorbic Acid at Biological Samples |
| title_short | Mean Centered Kinetic—Spectrophotometric Data—Continuous Wavelet Transform for Simultaneous Determination of Dopamine and Uric Acid in Presence of Ascorbic Acid at Biological Samples |
| title_sort | mean centered kinetic spectrophotometric data continuous wavelet transform for simultaneous determination of dopamine and uric acid in presence of ascorbic acid at biological samples |
| topic | kinetic simultaneous determination uric acid dopamine CWT |
| url | https://www.mdpi.com/2297-8739/9/10/282 |
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