Medicinal Plants <i>Galega officinalis</i> L. and Yacon Leaves as Potential Sources of Antidiabetic Drugs
Hypoglycemic and antioxidant properties of extracts of medicinal plants <i>Galega officinalis</i> L. (aboveground part) and yacon (<i>Smallanthus sonchifolius</i> Poepp. & Endl.) (leaves) as potential sources of biologically active substances with antidiabetic action have...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/9/1362 |
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| author | Halyna Hachkova Mariia Nagalievska Zoriana Soliljak Olena Kanyuka Alicja Zofia Kucharska Anna Sokół-Łętowska Elena Belonovskaya Vyacheslav Buko Nataliia Sybirna |
| author_facet | Halyna Hachkova Mariia Nagalievska Zoriana Soliljak Olena Kanyuka Alicja Zofia Kucharska Anna Sokół-Łętowska Elena Belonovskaya Vyacheslav Buko Nataliia Sybirna |
| author_sort | Halyna Hachkova |
| collection | DOAJ |
| description | Hypoglycemic and antioxidant properties of extracts of medicinal plants <i>Galega officinalis</i> L. (aboveground part) and yacon (<i>Smallanthus sonchifolius</i> Poepp. & Endl.) (leaves) as potential sources of biologically active substances with antidiabetic action have been studied. The pronounced hypoglycemic effect of <i>Galega officinalis</i> extract, devoid of alkaloids, at a dose of 600 mg/kg in experimental diabetes mellitus (DM) has been proven. The established effect is evidenced by a decrease in the concentration of glucose and glycosylated hemoglobin in the blood, increase glucose tolerance of cells, increase C-peptide and insulin content in the plasma of rats’ blood. The effective hypoglycemic effect of the extract in the studied pathology was confirmed by histological examination of the pancreas. The cytoprotective effect of the studied extract on pancreatic cells at a dose of 1200 mg/kg was experimentally confirmed. In the standard cut area, an increase was found in the number of Langerhans islets, their average area, diameter, volume, and a number of β-cells relative to these indicators in animals with diabetes. Comparative screening of the antioxidant properties of 30, 50, 70, and 96% water–ethanol extracts of yacon indicates the highest potential of 50% water-ethanol extract to block free radicals in in vitro model experiments. The non-alkaloid fraction of <i>Galega officinalis</i> extract showed moderate antioxidant activity and was inferior to yacon extract in its ability to neutralize reactive oxygen species (ROS) and bind metal ions of variable valence. The level of antioxidant potential of the studied extracts is due to differences in the quantitative content of compounds of phenolic nature in their compositions. The obtained data on the biological effects of <i>Galega officinalis</i> extract on the structural and functional state of β-cells of the pancreas and antioxidant properties of <i>Galega officinalis</i> and yacon extracts substantiate the prospects of using these plants to create antidiabetic medicines and functional foods based on them. |
| first_indexed | 2024-03-10T07:56:49Z |
| format | Article |
| id | doaj.art-1b12539cb6ba4655a92d3549cc11eb54 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T07:56:49Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-1b12539cb6ba4655a92d3549cc11eb542023-11-22T11:47:36ZengMDPI AGAntioxidants2076-39212021-08-01109136210.3390/antiox10091362Medicinal Plants <i>Galega officinalis</i> L. and Yacon Leaves as Potential Sources of Antidiabetic DrugsHalyna Hachkova0Mariia Nagalievska1Zoriana Soliljak2Olena Kanyuka3Alicja Zofia Kucharska4Anna Sokół-Łętowska5Elena Belonovskaya6Vyacheslav Buko7Nataliia Sybirna8Department of Biochemistry, Faculty of the Biology, Ivan Franko National University of Lviv, Hrushevskyi St. 4, 79005 Lviv, UkraineDepartment of Biochemistry, Faculty of the Biology, Ivan Franko National University of Lviv, Hrushevskyi St. 4, 79005 Lviv, UkraineDepartment of Biochemistry, Faculty of the Biology, Ivan Franko National University of Lviv, Hrushevskyi St. 4, 79005 Lviv, UkraineDepartment of Biochemistry, Faculty of the Biology, Ivan Franko National University of Lviv, Hrushevskyi St. 4, 79005 Lviv, UkraineDepartment of Fruit, Vegetable and Plant Nutraceutical Technology, Faculty of the Biotechnology and Food Science, Wrocław University of Environmental and Life Sciences, Chełmońskiego St. 37, 51-630 Wrocław, PolandDepartment of Fruit, Vegetable and Plant Nutraceutical Technology, Faculty of the Biotechnology and Food Science, Wrocław University of Environmental and Life Sciences, Chełmońskiego St. 37, 51-630 Wrocław, PolandDivision of Biochemical Pharmacology, Institute of Biochemistry of Biologically Active Compounds, National Academy of Sciences, BLK, 50, 230030 Grodno, BelarusDivision of Biochemical Pharmacology, Institute of Biochemistry of Biologically Active Compounds, National Academy of Sciences, BLK, 50, 230030 Grodno, BelarusDepartment of Biochemistry, Faculty of the Biology, Ivan Franko National University of Lviv, Hrushevskyi St. 4, 79005 Lviv, UkraineHypoglycemic and antioxidant properties of extracts of medicinal plants <i>Galega officinalis</i> L. (aboveground part) and yacon (<i>Smallanthus sonchifolius</i> Poepp. & Endl.) (leaves) as potential sources of biologically active substances with antidiabetic action have been studied. The pronounced hypoglycemic effect of <i>Galega officinalis</i> extract, devoid of alkaloids, at a dose of 600 mg/kg in experimental diabetes mellitus (DM) has been proven. The established effect is evidenced by a decrease in the concentration of glucose and glycosylated hemoglobin in the blood, increase glucose tolerance of cells, increase C-peptide and insulin content in the plasma of rats’ blood. The effective hypoglycemic effect of the extract in the studied pathology was confirmed by histological examination of the pancreas. The cytoprotective effect of the studied extract on pancreatic cells at a dose of 1200 mg/kg was experimentally confirmed. In the standard cut area, an increase was found in the number of Langerhans islets, their average area, diameter, volume, and a number of β-cells relative to these indicators in animals with diabetes. Comparative screening of the antioxidant properties of 30, 50, 70, and 96% water–ethanol extracts of yacon indicates the highest potential of 50% water-ethanol extract to block free radicals in in vitro model experiments. The non-alkaloid fraction of <i>Galega officinalis</i> extract showed moderate antioxidant activity and was inferior to yacon extract in its ability to neutralize reactive oxygen species (ROS) and bind metal ions of variable valence. The level of antioxidant potential of the studied extracts is due to differences in the quantitative content of compounds of phenolic nature in their compositions. The obtained data on the biological effects of <i>Galega officinalis</i> extract on the structural and functional state of β-cells of the pancreas and antioxidant properties of <i>Galega officinalis</i> and yacon extracts substantiate the prospects of using these plants to create antidiabetic medicines and functional foods based on them.https://www.mdpi.com/2076-3921/10/9/1362diabetes mellitus<i>Galega officinalis</i> L.<i>Smallanthus sonchifolius</i> Poepp. and Endl.in vitro antioxidant activityhypoglycemic effectpancreatic β-cell |
| spellingShingle | Halyna Hachkova Mariia Nagalievska Zoriana Soliljak Olena Kanyuka Alicja Zofia Kucharska Anna Sokół-Łętowska Elena Belonovskaya Vyacheslav Buko Nataliia Sybirna Medicinal Plants <i>Galega officinalis</i> L. and Yacon Leaves as Potential Sources of Antidiabetic Drugs Antioxidants diabetes mellitus <i>Galega officinalis</i> L. <i>Smallanthus sonchifolius</i> Poepp. and Endl. in vitro antioxidant activity hypoglycemic effect pancreatic β-cell |
| title | Medicinal Plants <i>Galega officinalis</i> L. and Yacon Leaves as Potential Sources of Antidiabetic Drugs |
| title_full | Medicinal Plants <i>Galega officinalis</i> L. and Yacon Leaves as Potential Sources of Antidiabetic Drugs |
| title_fullStr | Medicinal Plants <i>Galega officinalis</i> L. and Yacon Leaves as Potential Sources of Antidiabetic Drugs |
| title_full_unstemmed | Medicinal Plants <i>Galega officinalis</i> L. and Yacon Leaves as Potential Sources of Antidiabetic Drugs |
| title_short | Medicinal Plants <i>Galega officinalis</i> L. and Yacon Leaves as Potential Sources of Antidiabetic Drugs |
| title_sort | medicinal plants i galega officinalis i l and yacon leaves as potential sources of antidiabetic drugs |
| topic | diabetes mellitus <i>Galega officinalis</i> L. <i>Smallanthus sonchifolius</i> Poepp. and Endl. in vitro antioxidant activity hypoglycemic effect pancreatic β-cell |
| url | https://www.mdpi.com/2076-3921/10/9/1362 |
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