Direct somatic embryogenesis and related gene expression networks in leaf explants of Hippeastrum ‘Bangkok Rose’

Hippeastrum, a highly diverse genus in the Amaryllidaceae family, is a valuable ornamental bulbous flowering plant. Somatic embryogenesis (SE) is an efficient method for mass production of Hippeastrum plantlets. Previous studies have been devoted to the in vitro propagation of Hippeastrum, but the S...

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Main Authors: Jingjue Zeng, Yi Deng, Shahid Iqbal, Jiarui Zhang, Kunlin Wu, Guohua Ma, Lin Li, Guangyi Dai, Rufang Deng, Lin Fang, Songjun Zeng
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2024-03-01
Series:Horticultural Plant Journal
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2468014123001759
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author Jingjue Zeng
Yi Deng
Shahid Iqbal
Jiarui Zhang
Kunlin Wu
Guohua Ma
Lin Li
Guangyi Dai
Rufang Deng
Lin Fang
Songjun Zeng
author_facet Jingjue Zeng
Yi Deng
Shahid Iqbal
Jiarui Zhang
Kunlin Wu
Guohua Ma
Lin Li
Guangyi Dai
Rufang Deng
Lin Fang
Songjun Zeng
author_sort Jingjue Zeng
collection DOAJ
description Hippeastrum, a highly diverse genus in the Amaryllidaceae family, is a valuable ornamental bulbous flowering plant. Somatic embryogenesis (SE) is an efficient method for mass production of Hippeastrum plantlets. Previous studies have been devoted to the in vitro propagation of Hippeastrum, but the SE and its regulatory networks are rarely reported. In this study, we established a direct SE method of Hippeastrum ‘Bangkok Rose’ using leaf bases as explants. MS supplemented with 1.00 mg · L−1 NAA +1.00 mg · L−1 KT + 0.25 mg · L−1 TDZ was the optimal medium for SE. Histological observations showed that the bipolar somatic embryo originated from the epidermal cell layer and underwent initiation, globular, scutellar and coleoptile stages. During SE, endogenous hormones of IAA, CTK, ABA, and SA were highly accumulated. Transcriptomic analysis revealed the genes encoding auxin biosynthesis/metabolic enzymes and efflux carriers were induced, while the auxin receptor of TIR1 and ARF transcriptional repressor of Aux/IAA were down-regulated and up-regulated, respectively, leading to suppression of auxin signaling. In contrast, cytokine signaling was promoted at the early stage of SE, as biosynthesis, transport, and signaling components were up-regulated. Various stress-related genes were up-regulated at the early or late stages of SE. Chromatin remodeling could also be dynamically regulated via distinct expression enzymes that control histone methylation and acetylation during SE. Moreover, key SE regulators, including WOXs and SERKs were highly expressed along with SE. Overall, the present study provides insights into the SE regulatory mechanisms of the Hippeastrum.
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spelling doaj.art-1b12defab5e9421ca344484719a0fc782024-03-07T05:28:51ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412024-03-01102556572Direct somatic embryogenesis and related gene expression networks in leaf explants of Hippeastrum ‘Bangkok Rose’Jingjue Zeng0Yi Deng1Shahid Iqbal2Jiarui Zhang3Kunlin Wu4Guohua Ma5Lin Li6Guangyi Dai7Rufang Deng8Lin Fang9Songjun Zeng10Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; University of the Chinese Academy of Sciences, Beijing 100049, China; South China National Botanical Garden, Guangzhou, Guangdong 510650, ChinaZhaoqing Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Zhaoqing, Guangdong 526000, ChinaHorticultural Science Department, North Florida Research and Education Center, University of Florida/IFAS, Quincy, FL 32351, USAGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; University of the Chinese Academy of Sciences, Beijing 100049, China; South China National Botanical Garden, Guangzhou, Guangdong 510650, ChinaGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; South China National Botanical Garden, Guangzhou, Guangdong 510650, ChinaGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; South China National Botanical Garden, Guangzhou, Guangdong 510650, ChinaGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; South China National Botanical Garden, Guangzhou, Guangdong 510650, ChinaPublic Laboratory of South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; South China National Botanical Garden, Guangzhou, Guangdong 510650, ChinaPublic Laboratory of South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; South China National Botanical Garden, Guangzhou, Guangdong 510650, ChinaGuangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; South China National Botanical Garden, Guangzhou, Guangdong 510650, China; Corresponding authors.Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; South China National Botanical Garden, Guangzhou, Guangdong 510650, China; Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong 510650, China; Corresponding authors.Hippeastrum, a highly diverse genus in the Amaryllidaceae family, is a valuable ornamental bulbous flowering plant. Somatic embryogenesis (SE) is an efficient method for mass production of Hippeastrum plantlets. Previous studies have been devoted to the in vitro propagation of Hippeastrum, but the SE and its regulatory networks are rarely reported. In this study, we established a direct SE method of Hippeastrum ‘Bangkok Rose’ using leaf bases as explants. MS supplemented with 1.00 mg · L−1 NAA +1.00 mg · L−1 KT + 0.25 mg · L−1 TDZ was the optimal medium for SE. Histological observations showed that the bipolar somatic embryo originated from the epidermal cell layer and underwent initiation, globular, scutellar and coleoptile stages. During SE, endogenous hormones of IAA, CTK, ABA, and SA were highly accumulated. Transcriptomic analysis revealed the genes encoding auxin biosynthesis/metabolic enzymes and efflux carriers were induced, while the auxin receptor of TIR1 and ARF transcriptional repressor of Aux/IAA were down-regulated and up-regulated, respectively, leading to suppression of auxin signaling. In contrast, cytokine signaling was promoted at the early stage of SE, as biosynthesis, transport, and signaling components were up-regulated. Various stress-related genes were up-regulated at the early or late stages of SE. Chromatin remodeling could also be dynamically regulated via distinct expression enzymes that control histone methylation and acetylation during SE. Moreover, key SE regulators, including WOXs and SERKs were highly expressed along with SE. Overall, the present study provides insights into the SE regulatory mechanisms of the Hippeastrum.http://www.sciencedirect.com/science/article/pii/S2468014123001759HippeastrumTissue cultureSomatic embryogenesisGene regulation
spellingShingle Jingjue Zeng
Yi Deng
Shahid Iqbal
Jiarui Zhang
Kunlin Wu
Guohua Ma
Lin Li
Guangyi Dai
Rufang Deng
Lin Fang
Songjun Zeng
Direct somatic embryogenesis and related gene expression networks in leaf explants of Hippeastrum ‘Bangkok Rose’
Horticultural Plant Journal
Hippeastrum
Tissue culture
Somatic embryogenesis
Gene regulation
title Direct somatic embryogenesis and related gene expression networks in leaf explants of Hippeastrum ‘Bangkok Rose’
title_full Direct somatic embryogenesis and related gene expression networks in leaf explants of Hippeastrum ‘Bangkok Rose’
title_fullStr Direct somatic embryogenesis and related gene expression networks in leaf explants of Hippeastrum ‘Bangkok Rose’
title_full_unstemmed Direct somatic embryogenesis and related gene expression networks in leaf explants of Hippeastrum ‘Bangkok Rose’
title_short Direct somatic embryogenesis and related gene expression networks in leaf explants of Hippeastrum ‘Bangkok Rose’
title_sort direct somatic embryogenesis and related gene expression networks in leaf explants of hippeastrum bangkok rose
topic Hippeastrum
Tissue culture
Somatic embryogenesis
Gene regulation
url http://www.sciencedirect.com/science/article/pii/S2468014123001759
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