Epitranscriptome profiling of spleen mRNA m6A methylation reveals pathways of host responses to malaria parasite infection
N6-Methyladenosine (m6A), the most abundant mammalian mRNA modification, has been reported to modulate various viral infections. Although it has been confirmed that RNA modifications can also modulate the replication and development of different parasites, the role of the RNA epitranscriptome in the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.998756/full |
| _version_ | 1811183707274346496 |
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| author | Luoluo Wang Jian Wu Runzhou Liu Wenjun Chen Zhichang Pang Fan Zhou Lu Xia Lu Xia Jia Huang Tao Pan Xin-zhuan Su Xiaoyun Wang |
| author_facet | Luoluo Wang Jian Wu Runzhou Liu Wenjun Chen Zhichang Pang Fan Zhou Lu Xia Lu Xia Jia Huang Tao Pan Xin-zhuan Su Xiaoyun Wang |
| author_sort | Luoluo Wang |
| collection | DOAJ |
| description | N6-Methyladenosine (m6A), the most abundant mammalian mRNA modification, has been reported to modulate various viral infections. Although it has been confirmed that RNA modifications can also modulate the replication and development of different parasites, the role of the RNA epitranscriptome in the regulation of host response post parasite infection remains to be elucidated. Here we report host spleen m6A epitranscriptome landscapes induced by different strains of the malaria parasite Plasmodium yoelii. We found that malaria parasite infection dramatically changes host spleen m6A mRNA modification and gene expression. Additionally, malaria parasite infection reprograms host immune response pathways by regulating the m6A modification enzymes. Collectively, our study is the first characterization of host spleen m6A methylome triggered by malaria parasite infections, and our data identify m6A modifications as significant transcriptome-wide marks during host-parasite interactions. We demonstrate that host mRNA methylation machinery can sense and respond to malaria parasite infections, and provide new insights into epitranscriptomic mechanisms underlying parasite-induced pathogenesis. |
| first_indexed | 2024-04-11T09:51:30Z |
| format | Article |
| id | doaj.art-1b142fbed064436d92121f308dd433fa |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-11T09:51:30Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-1b142fbed064436d92121f308dd433fa2022-12-22T04:30:47ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-09-011310.3389/fimmu.2022.998756998756Epitranscriptome profiling of spleen mRNA m6A methylation reveals pathways of host responses to malaria parasite infectionLuoluo Wang0Jian Wu1Runzhou Liu2Wenjun Chen3Zhichang Pang4Fan Zhou5Lu Xia6Lu Xia7Jia Huang8Tao Pan9Xin-zhuan Su10Xiaoyun Wang11School of Life Sciences, South China Normal University, Guangzhou, ChinaLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, United StatesSchool of Life Sciences, South China Normal University, Guangzhou, ChinaDepartment of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, IL, United StatesSchool of Life Sciences, South China Normal University, Guangzhou, ChinaSchool of Life Sciences, South China Normal University, Guangzhou, ChinaLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, United StatesCenter for Medical Genetics, School of Life Sciences, Central South University, Changsha, ChinaSchool of Life Sciences, South China Normal University, Guangzhou, ChinaDepartment of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL, United StatesLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, United StatesSchool of Life Sciences, South China Normal University, Guangzhou, ChinaN6-Methyladenosine (m6A), the most abundant mammalian mRNA modification, has been reported to modulate various viral infections. Although it has been confirmed that RNA modifications can also modulate the replication and development of different parasites, the role of the RNA epitranscriptome in the regulation of host response post parasite infection remains to be elucidated. Here we report host spleen m6A epitranscriptome landscapes induced by different strains of the malaria parasite Plasmodium yoelii. We found that malaria parasite infection dramatically changes host spleen m6A mRNA modification and gene expression. Additionally, malaria parasite infection reprograms host immune response pathways by regulating the m6A modification enzymes. Collectively, our study is the first characterization of host spleen m6A methylome triggered by malaria parasite infections, and our data identify m6A modifications as significant transcriptome-wide marks during host-parasite interactions. We demonstrate that host mRNA methylation machinery can sense and respond to malaria parasite infections, and provide new insights into epitranscriptomic mechanisms underlying parasite-induced pathogenesis.https://www.frontiersin.org/articles/10.3389/fimmu.2022.998756/fullRNA epitranscriptomeN6-methyladenosinemalaria infectionimmune responseplasmodium yoelii |
| spellingShingle | Luoluo Wang Jian Wu Runzhou Liu Wenjun Chen Zhichang Pang Fan Zhou Lu Xia Lu Xia Jia Huang Tao Pan Xin-zhuan Su Xiaoyun Wang Epitranscriptome profiling of spleen mRNA m6A methylation reveals pathways of host responses to malaria parasite infection Frontiers in Immunology RNA epitranscriptome N6-methyladenosine malaria infection immune response plasmodium yoelii |
| title | Epitranscriptome profiling of spleen mRNA m6A methylation reveals pathways of host responses to malaria parasite infection |
| title_full | Epitranscriptome profiling of spleen mRNA m6A methylation reveals pathways of host responses to malaria parasite infection |
| title_fullStr | Epitranscriptome profiling of spleen mRNA m6A methylation reveals pathways of host responses to malaria parasite infection |
| title_full_unstemmed | Epitranscriptome profiling of spleen mRNA m6A methylation reveals pathways of host responses to malaria parasite infection |
| title_short | Epitranscriptome profiling of spleen mRNA m6A methylation reveals pathways of host responses to malaria parasite infection |
| title_sort | epitranscriptome profiling of spleen mrna m6a methylation reveals pathways of host responses to malaria parasite infection |
| topic | RNA epitranscriptome N6-methyladenosine malaria infection immune response plasmodium yoelii |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.998756/full |
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