Inhibition of oil tea anthracnose by natural product extracts from Bacillus and Pseudoalteromonas isolates from mangrove soil
This study investigates the potential of natural products derived from two mangrove soil bacteria, GP521A and GP3R5, in anthracnose management in oil tea. Based on 16s rDNA sequencing, GP521A and GP3R5 were identified as Bacillus velezensis and Pseudoalteromonas caenipelagi, respectively. Both bacte...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1299118/full |
| _version_ | 1797628068518428672 |
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| author | Liwang Fei Liwang Fei Liwang Fei Xiaowen Xu Jinying Feng Lingyun Hao Lingyun Hao |
| author_facet | Liwang Fei Liwang Fei Liwang Fei Xiaowen Xu Jinying Feng Lingyun Hao Lingyun Hao |
| author_sort | Liwang Fei |
| collection | DOAJ |
| description | This study investigates the potential of natural products derived from two mangrove soil bacteria, GP521A and GP3R5, in anthracnose management in oil tea. Based on 16s rDNA sequencing, GP521A and GP3R5 were identified as Bacillus velezensis and Pseudoalteromonas caenipelagi, respectively. Both bacteria showed strong antagonistic effects towards multiple fungal pathogens on dual cultural plates. The ethyl acetate extracts of fermentation cultures of GP521A (FEGP5) and GP3R5 (FEGP3) significantly inhibited mycelial growth of both Colletotrichum camelliae MC171, C. fructicola CF-1 and Pyricularia oryzae P131 at 100 and 200 μg/mL, respectively. Furthermore, FEGP5 and FEGP3 significantly inhibited conidiation, conidial germination and appressorial formation in C. camelliae MC171 at 50 μg/mL, with stronger inhibitory effects observed in the latter. On detached oil tea leaves, 100 μg/mL of FEGP5 and FEGP3 resulted in ~ 98.0% and 97.5% reduction in diseased areas, respectively, when applied before MC171 inoculation, whereas the reduction was approximately 55.0% and 77.6%, respectively, in the curative treatments. In fresh fruits, both extracts also significantly inhibited disease development, either in preventative or curative treatments. Moreover, FEGP5 and FEGP3 demonstrated significantly lower aquatic toxicity than prochloraz at the same concentrations towards a commonly used bioindicator, Artemia salina. In conclusion, this study demonstrates the potential of marine microbes derived natural products in development of environmentally compatible biopesticides to control anthracnose in oil tea. |
| first_indexed | 2024-03-11T10:33:18Z |
| format | Article |
| id | doaj.art-1b15fa6fce864a14bd7123d542d63b47 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-03-11T10:33:18Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-1b15fa6fce864a14bd7123d542d63b472023-11-14T11:41:49ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-11-011010.3389/fmars.2023.12991181299118Inhibition of oil tea anthracnose by natural product extracts from Bacillus and Pseudoalteromonas isolates from mangrove soilLiwang Fei0Liwang Fei1Liwang Fei2Xiaowen Xu3Jinying Feng4Lingyun Hao5Lingyun Hao6Shenzhen Key Laboratory of Marine Bioresource & Eco-Environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaCollege of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, ChinaCenter for Plant Environmental Sensing, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaHubei Academy of Forestry, Wuhan, Hubei, ChinaShenzhen Key Laboratory of Marine Bioresource & Eco-Environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaShenzhen Key Laboratory of Marine Bioresource & Eco-Environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaCenter for Plant Environmental Sensing, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaThis study investigates the potential of natural products derived from two mangrove soil bacteria, GP521A and GP3R5, in anthracnose management in oil tea. Based on 16s rDNA sequencing, GP521A and GP3R5 were identified as Bacillus velezensis and Pseudoalteromonas caenipelagi, respectively. Both bacteria showed strong antagonistic effects towards multiple fungal pathogens on dual cultural plates. The ethyl acetate extracts of fermentation cultures of GP521A (FEGP5) and GP3R5 (FEGP3) significantly inhibited mycelial growth of both Colletotrichum camelliae MC171, C. fructicola CF-1 and Pyricularia oryzae P131 at 100 and 200 μg/mL, respectively. Furthermore, FEGP5 and FEGP3 significantly inhibited conidiation, conidial germination and appressorial formation in C. camelliae MC171 at 50 μg/mL, with stronger inhibitory effects observed in the latter. On detached oil tea leaves, 100 μg/mL of FEGP5 and FEGP3 resulted in ~ 98.0% and 97.5% reduction in diseased areas, respectively, when applied before MC171 inoculation, whereas the reduction was approximately 55.0% and 77.6%, respectively, in the curative treatments. In fresh fruits, both extracts also significantly inhibited disease development, either in preventative or curative treatments. Moreover, FEGP5 and FEGP3 demonstrated significantly lower aquatic toxicity than prochloraz at the same concentrations towards a commonly used bioindicator, Artemia salina. In conclusion, this study demonstrates the potential of marine microbes derived natural products in development of environmentally compatible biopesticides to control anthracnose in oil tea.https://www.frontiersin.org/articles/10.3389/fmars.2023.1299118/fullCamellia oleiferaanthracnosemangrove soil bacteriaBacillusPseudoalteromonasnatural product extracts |
| spellingShingle | Liwang Fei Liwang Fei Liwang Fei Xiaowen Xu Jinying Feng Lingyun Hao Lingyun Hao Inhibition of oil tea anthracnose by natural product extracts from Bacillus and Pseudoalteromonas isolates from mangrove soil Frontiers in Marine Science Camellia oleifera anthracnose mangrove soil bacteria Bacillus Pseudoalteromonas natural product extracts |
| title | Inhibition of oil tea anthracnose by natural product extracts from Bacillus and Pseudoalteromonas isolates from mangrove soil |
| title_full | Inhibition of oil tea anthracnose by natural product extracts from Bacillus and Pseudoalteromonas isolates from mangrove soil |
| title_fullStr | Inhibition of oil tea anthracnose by natural product extracts from Bacillus and Pseudoalteromonas isolates from mangrove soil |
| title_full_unstemmed | Inhibition of oil tea anthracnose by natural product extracts from Bacillus and Pseudoalteromonas isolates from mangrove soil |
| title_short | Inhibition of oil tea anthracnose by natural product extracts from Bacillus and Pseudoalteromonas isolates from mangrove soil |
| title_sort | inhibition of oil tea anthracnose by natural product extracts from bacillus and pseudoalteromonas isolates from mangrove soil |
| topic | Camellia oleifera anthracnose mangrove soil bacteria Bacillus Pseudoalteromonas natural product extracts |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1299118/full |
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