Ultrasensitive Determination of Glial-Fibrillary-Acidic-Protein (GFAP) in Human Serum-Matrix with a Label-Free Impedimetric Immunosensor
In this work, immobilizing anti-GFAP antibodies via covalent attachment onto L-cysteine/gold nanoparticles that were modified with screen-printed carbon electrodes (Anti-GFAP/L-cys/AuNps/SPCE) resulted in the development of a sensitive label-free impedance immunosensor for the detection of Glial Fib...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/12/1165 |
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| author | Goksu Ozcelikay Fariba Mollarasouli Mehmet Altay Unal Kıvılcım Gucuyener Sibel A. Ozkan |
| author_facet | Goksu Ozcelikay Fariba Mollarasouli Mehmet Altay Unal Kıvılcım Gucuyener Sibel A. Ozkan |
| author_sort | Goksu Ozcelikay |
| collection | DOAJ |
| description | In this work, immobilizing anti-GFAP antibodies via covalent attachment onto L-cysteine/gold nanoparticles that were modified with screen-printed carbon electrodes (Anti-GFAP/L-cys/AuNps/SPCE) resulted in the development of a sensitive label-free impedance immunosensor for the detection of Glial Fibrillary Acidic Protein (GFAP). The immunosensor’s stepwise construction was studied using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). L-cysteine was chosen as the linker between GFAP antibodies and Au NPs/SPCE because it enables the guided and stable immobilization of GFAP antibodies, thus resulting in increased immunosensor sensitivity. As a redox probe, 5 mM of [Fe(CN)6]3−/4− was used to measure the electron–transfer resistance (Ret), which was raised by the binding of antigens to the immobilized anti-GFAP on the surface of the modified electrode. A linear correlation between Rct and GFAP concentration was achieved under optimum conditions in the range of 1.0–1000.0 pg/mL, with an extraordinarily low detection limit of 51.0 fg/mL. The suggested immunosensor was successfully used to detect the presence of GFAP in human blood serum samples, yielding good findings. As a result, the proposed platform may be utilized to monitor central nervous system injuries. |
| first_indexed | 2024-03-09T17:15:58Z |
| format | Article |
| id | doaj.art-1b186b6bc5dc40649a0c26ebf4694381 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T17:15:58Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-1b186b6bc5dc40649a0c26ebf46943812023-11-24T13:37:43ZengMDPI AGBiosensors2079-63742022-12-011212116510.3390/bios12121165Ultrasensitive Determination of Glial-Fibrillary-Acidic-Protein (GFAP) in Human Serum-Matrix with a Label-Free Impedimetric ImmunosensorGoksu Ozcelikay0Fariba Mollarasouli1Mehmet Altay Unal2Kıvılcım Gucuyener3Sibel A. Ozkan4Department of Analytical Chemistry, Ankara University, Ankara 06560, TurkeyDepartment of Chemistry, Yasouj University, Yasouj 75918-74831, IranStem Cell Institute, Ankara University, Ankara 06520, TurkeyDepartment of Pediatric Neurology, Gazi University, Ankara 06510, TurkeyDepartment of Analytical Chemistry, Ankara University, Ankara 06560, TurkeyIn this work, immobilizing anti-GFAP antibodies via covalent attachment onto L-cysteine/gold nanoparticles that were modified with screen-printed carbon electrodes (Anti-GFAP/L-cys/AuNps/SPCE) resulted in the development of a sensitive label-free impedance immunosensor for the detection of Glial Fibrillary Acidic Protein (GFAP). The immunosensor’s stepwise construction was studied using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). L-cysteine was chosen as the linker between GFAP antibodies and Au NPs/SPCE because it enables the guided and stable immobilization of GFAP antibodies, thus resulting in increased immunosensor sensitivity. As a redox probe, 5 mM of [Fe(CN)6]3−/4− was used to measure the electron–transfer resistance (Ret), which was raised by the binding of antigens to the immobilized anti-GFAP on the surface of the modified electrode. A linear correlation between Rct and GFAP concentration was achieved under optimum conditions in the range of 1.0–1000.0 pg/mL, with an extraordinarily low detection limit of 51.0 fg/mL. The suggested immunosensor was successfully used to detect the presence of GFAP in human blood serum samples, yielding good findings. As a result, the proposed platform may be utilized to monitor central nervous system injuries.https://www.mdpi.com/2079-6374/12/12/1165label-free impedance immunosensorscreen printed carbon electrode (SPCE)Au nanoparticles (Au NPs)glial fibrillary acidic protein (GFAP) |
| spellingShingle | Goksu Ozcelikay Fariba Mollarasouli Mehmet Altay Unal Kıvılcım Gucuyener Sibel A. Ozkan Ultrasensitive Determination of Glial-Fibrillary-Acidic-Protein (GFAP) in Human Serum-Matrix with a Label-Free Impedimetric Immunosensor Biosensors label-free impedance immunosensor screen printed carbon electrode (SPCE) Au nanoparticles (Au NPs) glial fibrillary acidic protein (GFAP) |
| title | Ultrasensitive Determination of Glial-Fibrillary-Acidic-Protein (GFAP) in Human Serum-Matrix with a Label-Free Impedimetric Immunosensor |
| title_full | Ultrasensitive Determination of Glial-Fibrillary-Acidic-Protein (GFAP) in Human Serum-Matrix with a Label-Free Impedimetric Immunosensor |
| title_fullStr | Ultrasensitive Determination of Glial-Fibrillary-Acidic-Protein (GFAP) in Human Serum-Matrix with a Label-Free Impedimetric Immunosensor |
| title_full_unstemmed | Ultrasensitive Determination of Glial-Fibrillary-Acidic-Protein (GFAP) in Human Serum-Matrix with a Label-Free Impedimetric Immunosensor |
| title_short | Ultrasensitive Determination of Glial-Fibrillary-Acidic-Protein (GFAP) in Human Serum-Matrix with a Label-Free Impedimetric Immunosensor |
| title_sort | ultrasensitive determination of glial fibrillary acidic protein gfap in human serum matrix with a label free impedimetric immunosensor |
| topic | label-free impedance immunosensor screen printed carbon electrode (SPCE) Au nanoparticles (Au NPs) glial fibrillary acidic protein (GFAP) |
| url | https://www.mdpi.com/2079-6374/12/12/1165 |
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