Evolutionary Analysis of the Melon (<i>Cucumis melo</i> L.) GH3 Gene Family and Identification of GH3 Genes Related to Fruit Growth and Development
The indole-3-acetic acid (IAA) auxin is an important endogenous hormone that plays a key role in the regulation of plant growth and development. In recent years, with the progression of auxin-related research, the function of the Gretchen Hagen 3 (GH3) gene has become a prominent research topic. How...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/6/1382 |
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| author | Sheng Chen Kaiqin Zhong Yongyu Li Changhui Bai Zhuzheng Xue Yufen Wu |
| author_facet | Sheng Chen Kaiqin Zhong Yongyu Li Changhui Bai Zhuzheng Xue Yufen Wu |
| author_sort | Sheng Chen |
| collection | DOAJ |
| description | The indole-3-acetic acid (IAA) auxin is an important endogenous hormone that plays a key role in the regulation of plant growth and development. In recent years, with the progression of auxin-related research, the function of the Gretchen Hagen 3 (GH3) gene has become a prominent research topic. However, studies focusing on the characteristics and functions of melon GH3 family genes are still lacking. This study presents a systematic identification of melon GH3 gene family members based on genomic data. The evolution of melon GH3 family genes was systematically analyzed by means of bioinformatics, and the expression patterns of the GH3 family genes in different melon tissues during different fruit developmental stages and with various levels of 1-naphthaleneacetic acid (NAA) induction were analyzed with transcriptomics and RT-qPCR. The melon genome contains 10 GH3 genes distributed across seven chromosomes, and most of these genes are expressed in the plasma membrane. According to evolutionary analysis and the number of GH3 family genes, these genes can be divided into three subgroups, and they have been conserved throughout the evolution of melon. The melon GH3 gene has a wide range of expression patterns across distinct tissue types, with expression generally being higher in flowers and fruit. Through promoter analysis, we found that most <i>cis</i>-acting elements contained light- and IAA-responsive elements. Based on the RNA-seq and RT-qPCR analyses, it can be speculated that <i>CmGH3-5</i>, <i>CmGH3-6</i> and <i>CmGH3-7</i> may be involved in the process of melon fruit development. In conclusion, our findings suggest that the GH3 gene family plays an important role in the development of melon fruit. This study provides an important theoretical basis for further research on the function of the GH3 gene family and the molecular mechanism underlying the development of melon fruit. |
| first_indexed | 2024-03-11T05:59:54Z |
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| language | English |
| last_indexed | 2024-03-11T05:59:54Z |
| publishDate | 2023-03-01 |
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| series | Plants |
| spelling | doaj.art-1b19aaae288d49f791789d4ed585d1ce2023-11-17T13:23:09ZengMDPI AGPlants2223-77472023-03-01126138210.3390/plants12061382Evolutionary Analysis of the Melon (<i>Cucumis melo</i> L.) GH3 Gene Family and Identification of GH3 Genes Related to Fruit Growth and DevelopmentSheng Chen0Kaiqin Zhong1Yongyu Li2Changhui Bai3Zhuzheng Xue4Yufen Wu5Agricultural Bioresources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, ChinaFuzhou Institute of Vegetable Science, Fuzhou 350018, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCrops Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaCrops Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaAgricultural Bioresources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, ChinaThe indole-3-acetic acid (IAA) auxin is an important endogenous hormone that plays a key role in the regulation of plant growth and development. In recent years, with the progression of auxin-related research, the function of the Gretchen Hagen 3 (GH3) gene has become a prominent research topic. However, studies focusing on the characteristics and functions of melon GH3 family genes are still lacking. This study presents a systematic identification of melon GH3 gene family members based on genomic data. The evolution of melon GH3 family genes was systematically analyzed by means of bioinformatics, and the expression patterns of the GH3 family genes in different melon tissues during different fruit developmental stages and with various levels of 1-naphthaleneacetic acid (NAA) induction were analyzed with transcriptomics and RT-qPCR. The melon genome contains 10 GH3 genes distributed across seven chromosomes, and most of these genes are expressed in the plasma membrane. According to evolutionary analysis and the number of GH3 family genes, these genes can be divided into three subgroups, and they have been conserved throughout the evolution of melon. The melon GH3 gene has a wide range of expression patterns across distinct tissue types, with expression generally being higher in flowers and fruit. Through promoter analysis, we found that most <i>cis</i>-acting elements contained light- and IAA-responsive elements. Based on the RNA-seq and RT-qPCR analyses, it can be speculated that <i>CmGH3-5</i>, <i>CmGH3-6</i> and <i>CmGH3-7</i> may be involved in the process of melon fruit development. In conclusion, our findings suggest that the GH3 gene family plays an important role in the development of melon fruit. This study provides an important theoretical basis for further research on the function of the GH3 gene family and the molecular mechanism underlying the development of melon fruit.https://www.mdpi.com/2223-7747/12/6/1382evolutionary analysisexpression analysisfruit developmentGH3 gene familymelon |
| spellingShingle | Sheng Chen Kaiqin Zhong Yongyu Li Changhui Bai Zhuzheng Xue Yufen Wu Evolutionary Analysis of the Melon (<i>Cucumis melo</i> L.) GH3 Gene Family and Identification of GH3 Genes Related to Fruit Growth and Development Plants evolutionary analysis expression analysis fruit development GH3 gene family melon |
| title | Evolutionary Analysis of the Melon (<i>Cucumis melo</i> L.) GH3 Gene Family and Identification of GH3 Genes Related to Fruit Growth and Development |
| title_full | Evolutionary Analysis of the Melon (<i>Cucumis melo</i> L.) GH3 Gene Family and Identification of GH3 Genes Related to Fruit Growth and Development |
| title_fullStr | Evolutionary Analysis of the Melon (<i>Cucumis melo</i> L.) GH3 Gene Family and Identification of GH3 Genes Related to Fruit Growth and Development |
| title_full_unstemmed | Evolutionary Analysis of the Melon (<i>Cucumis melo</i> L.) GH3 Gene Family and Identification of GH3 Genes Related to Fruit Growth and Development |
| title_short | Evolutionary Analysis of the Melon (<i>Cucumis melo</i> L.) GH3 Gene Family and Identification of GH3 Genes Related to Fruit Growth and Development |
| title_sort | evolutionary analysis of the melon i cucumis melo i l gh3 gene family and identification of gh3 genes related to fruit growth and development |
| topic | evolutionary analysis expression analysis fruit development GH3 gene family melon |
| url | https://www.mdpi.com/2223-7747/12/6/1382 |
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