Integrated metabolome and transcriptome analysis identifies candidate genes involved in triterpenoid saponin biosynthesis in leaves of Centella asiatica (L.) Urban
Centella asiatica (L.) Urban is a well-known medicinal plant which has multiple pharmacological properties. Notably, the leaves of C. asiatica contain large amounts of triterpenoid saponins. However, there have only been a few studies systematically elucidating the metabolic dynamics and transcripti...
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Frontiers Media S.A.
2024-01-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1295186/full |
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| author | Lingyun Wan Lingyun Wan Lingyun Wan Qiulan Huang Cui Li Cui Li Haixia Yu Haixia Yu Haixia Yu Guiyu Tan Guiyu Tan Guiyu Tan Shugen Wei Shugen Wei Shugen Wei Ahmed H. El-Sappah Ahmed H. El-Sappah Suren Sooranna Suren Sooranna Kun Zhang Kun Zhang Kun Zhang Limei Pan Limei Pan Limei Pan Zhanjiang Zhang Zhanjiang Zhang Zhanjiang Zhang Ming Lei Ming Lei |
| author_facet | Lingyun Wan Lingyun Wan Lingyun Wan Qiulan Huang Cui Li Cui Li Haixia Yu Haixia Yu Haixia Yu Guiyu Tan Guiyu Tan Guiyu Tan Shugen Wei Shugen Wei Shugen Wei Ahmed H. El-Sappah Ahmed H. El-Sappah Suren Sooranna Suren Sooranna Kun Zhang Kun Zhang Kun Zhang Limei Pan Limei Pan Limei Pan Zhanjiang Zhang Zhanjiang Zhang Zhanjiang Zhang Ming Lei Ming Lei |
| author_sort | Lingyun Wan |
| collection | DOAJ |
| description | Centella asiatica (L.) Urban is a well-known medicinal plant which has multiple pharmacological properties. Notably, the leaves of C. asiatica contain large amounts of triterpenoid saponins. However, there have only been a few studies systematically elucidating the metabolic dynamics and transcriptional differences regarding triterpenoid saponin biosynthesis during the leaf development stages of C. asiatica. Here, we performed a comprehensive analysis of the metabolome and transcriptome to reveal the dynamic patterns of triterpenoid saponin accumulation and identified the key candidate genes associated with their biosynthesis in C. asiatica leaves. In this study, we found that the key precursors in the synthesis of terpenoids, including DMAPP, IPP and β-amyrin, as well as 22 triterpenes and eight triterpenoid saponins were considered as differentially accumulated metabolites. The concentrations of DMAPP, IPP and β-amyrin showed significant increases during the entire stage of leaf development. The levels of 12 triterpenes decreased only during the later stages of leaf development, but five triterpenoid saponins rapidly accumulated at the early stages, and later decreased to a constant level. Furthermore, 48 genes involved in the MVA, MEP and 2, 3-oxidosqualene biosynthetic pathways were selected following gene annotation. Then, 17 CYP450s and 26 UGTs, which are respectively responsible for backbone modifications, were used for phylogenetic-tree construction and time-specific expression analysis. From these data, by integrating metabolomics and transcriptomics analyses, we identified CaHDR1 and CaIDI2 as the candidate genes associated with DMAPP and IPP synthesis, respectively, and CaβAS1 as the one regulating β-amyrin synthesis. Two genes from the CYP716 family were confirmed as CaCYP716A83 and CaCYP716C11. We also selected two UGT73 families as candidate genes, associated with glycosylation of the terpenoid backbone at C-3 in C. asiatica. These findings will pave the way for further research on the molecular mechanisms associated with triterpenoid saponin biosynthesis in C. asiatica. |
| first_indexed | 2024-03-08T14:36:57Z |
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| language | English |
| last_indexed | 2024-03-08T14:36:57Z |
| publishDate | 2024-01-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-1b1a4f1ba21e4accb572e4ad1274ad102024-01-12T04:30:33ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-01-011410.3389/fpls.2023.12951861295186Integrated metabolome and transcriptome analysis identifies candidate genes involved in triterpenoid saponin biosynthesis in leaves of Centella asiatica (L.) UrbanLingyun Wan0Lingyun Wan1Lingyun Wan2Qiulan Huang3Cui Li4Cui Li5Haixia Yu6Haixia Yu7Haixia Yu8Guiyu Tan9Guiyu Tan10Guiyu Tan11Shugen Wei12Shugen Wei13Shugen Wei14Ahmed H. El-Sappah15Ahmed H. El-Sappah16Suren Sooranna17Suren Sooranna18Kun Zhang19Kun Zhang20Kun Zhang21Limei Pan22Limei Pan23Limei Pan24Zhanjiang Zhang25Zhanjiang Zhang26Zhanjiang Zhang27Ming Lei28Ming Lei29Guangxi Key Laboratory for High-Quality Formation and Utilization of Dao-Di Herbs, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Center for Traditional Chinese Medicine (TCM) Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for the Development of Southwestern Endangered Medicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaFaculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, ChinaNational Center for Traditional Chinese Medicine (TCM) Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for the Development of Southwestern Endangered Medicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaGuangxi Key Laboratory for High-Quality Formation and Utilization of Dao-Di Herbs, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Center for Traditional Chinese Medicine (TCM) Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for the Development of Southwestern Endangered Medicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaGuangxi Key Laboratory for High-Quality Formation and Utilization of Dao-Di Herbs, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Center for Traditional Chinese Medicine (TCM) Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for the Development of Southwestern Endangered Medicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaGuangxi Key Laboratory for High-Quality Formation and Utilization of Dao-Di Herbs, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Center for Traditional Chinese Medicine (TCM) Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for the Development of Southwestern Endangered Medicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaFaculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, ChinaGenetics Department, Faculty of Agriculture, Zagazig University, Zagazig, EgyptNational Engineering Research Center for the Development of Southwestern Endangered Medicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaDepartment of Metabolism, Digestion and Reproduction, Imperial College London, London, United KingdomGuangxi Key Laboratory for High-Quality Formation and Utilization of Dao-Di Herbs, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Center for Traditional Chinese Medicine (TCM) Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for the Development of Southwestern Endangered Medicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaGuangxi Key Laboratory for High-Quality Formation and Utilization of Dao-Di Herbs, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Center for Traditional Chinese Medicine (TCM) Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for the Development of Southwestern Endangered Medicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaGuangxi Key Laboratory for High-Quality Formation and Utilization of Dao-Di Herbs, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Center for Traditional Chinese Medicine (TCM) Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for the Development of Southwestern Endangered Medicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Center for Traditional Chinese Medicine (TCM) Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaNational Engineering Research Center for the Development of Southwestern Endangered Medicinal Materials, Guangxi Botanical Garden of Medicinal Plants, Nanning, ChinaCentella asiatica (L.) Urban is a well-known medicinal plant which has multiple pharmacological properties. Notably, the leaves of C. asiatica contain large amounts of triterpenoid saponins. However, there have only been a few studies systematically elucidating the metabolic dynamics and transcriptional differences regarding triterpenoid saponin biosynthesis during the leaf development stages of C. asiatica. Here, we performed a comprehensive analysis of the metabolome and transcriptome to reveal the dynamic patterns of triterpenoid saponin accumulation and identified the key candidate genes associated with their biosynthesis in C. asiatica leaves. In this study, we found that the key precursors in the synthesis of terpenoids, including DMAPP, IPP and β-amyrin, as well as 22 triterpenes and eight triterpenoid saponins were considered as differentially accumulated metabolites. The concentrations of DMAPP, IPP and β-amyrin showed significant increases during the entire stage of leaf development. The levels of 12 triterpenes decreased only during the later stages of leaf development, but five triterpenoid saponins rapidly accumulated at the early stages, and later decreased to a constant level. Furthermore, 48 genes involved in the MVA, MEP and 2, 3-oxidosqualene biosynthetic pathways were selected following gene annotation. Then, 17 CYP450s and 26 UGTs, which are respectively responsible for backbone modifications, were used for phylogenetic-tree construction and time-specific expression analysis. From these data, by integrating metabolomics and transcriptomics analyses, we identified CaHDR1 and CaIDI2 as the candidate genes associated with DMAPP and IPP synthesis, respectively, and CaβAS1 as the one regulating β-amyrin synthesis. Two genes from the CYP716 family were confirmed as CaCYP716A83 and CaCYP716C11. We also selected two UGT73 families as candidate genes, associated with glycosylation of the terpenoid backbone at C-3 in C. asiatica. These findings will pave the way for further research on the molecular mechanisms associated with triterpenoid saponin biosynthesis in C. asiatica.https://www.frontiersin.org/articles/10.3389/fpls.2023.1295186/fullCentella asiaticatranscriptomemetabolometriterpenoid saponincandidate gene |
| spellingShingle | Lingyun Wan Lingyun Wan Lingyun Wan Qiulan Huang Cui Li Cui Li Haixia Yu Haixia Yu Haixia Yu Guiyu Tan Guiyu Tan Guiyu Tan Shugen Wei Shugen Wei Shugen Wei Ahmed H. El-Sappah Ahmed H. El-Sappah Suren Sooranna Suren Sooranna Kun Zhang Kun Zhang Kun Zhang Limei Pan Limei Pan Limei Pan Zhanjiang Zhang Zhanjiang Zhang Zhanjiang Zhang Ming Lei Ming Lei Integrated metabolome and transcriptome analysis identifies candidate genes involved in triterpenoid saponin biosynthesis in leaves of Centella asiatica (L.) Urban Frontiers in Plant Science Centella asiatica transcriptome metabolome triterpenoid saponin candidate gene |
| title | Integrated metabolome and transcriptome analysis identifies candidate genes involved in triterpenoid saponin biosynthesis in leaves of Centella asiatica (L.) Urban |
| title_full | Integrated metabolome and transcriptome analysis identifies candidate genes involved in triterpenoid saponin biosynthesis in leaves of Centella asiatica (L.) Urban |
| title_fullStr | Integrated metabolome and transcriptome analysis identifies candidate genes involved in triterpenoid saponin biosynthesis in leaves of Centella asiatica (L.) Urban |
| title_full_unstemmed | Integrated metabolome and transcriptome analysis identifies candidate genes involved in triterpenoid saponin biosynthesis in leaves of Centella asiatica (L.) Urban |
| title_short | Integrated metabolome and transcriptome analysis identifies candidate genes involved in triterpenoid saponin biosynthesis in leaves of Centella asiatica (L.) Urban |
| title_sort | integrated metabolome and transcriptome analysis identifies candidate genes involved in triterpenoid saponin biosynthesis in leaves of centella asiatica l urban |
| topic | Centella asiatica transcriptome metabolome triterpenoid saponin candidate gene |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1295186/full |
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