The Effect of Endurance and Resistance Training on Apoptosis Activity and Collagen Deposition in Heart Tissue of Diabetic Rats
Background & objective: Diabetes causes chronic problems in the structure and function of tissues, such as apoptosis and fibrosis in addition to glycemic disorders. In this study the effect of 8 weeks of endurance and resistance training on various signaling pathways of apoptosis and tissue fibr...
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| Format: | Article |
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Ardabil University of Medical Sciences
2022-07-01
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| Series: | Journal of Ardabil University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jarums.arums.ac.ir/article-1-2194-en.html |
| _version_ | 1797950988518162432 |
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| author | Hamideh Montazery Taleghani Nader Shakeri Khosro Ebrahim Rahman Soori Mandana Gholami |
| author_facet | Hamideh Montazery Taleghani Nader Shakeri Khosro Ebrahim Rahman Soori Mandana Gholami |
| author_sort | Hamideh Montazery Taleghani |
| collection | DOAJ |
| description | Background & objective: Diabetes causes chronic problems in the structure and function of tissues, such as apoptosis and fibrosis in addition to glycemic disorders. In this study the effect of 8 weeks of endurance and resistance training on various signaling pathways of apoptosis and tissue fibrosis of the heart of diabetic rats was investigated.
Methods: Thirty Wistar rats, approximately 8-10 weeks old, weight about 210-250 grams, received intraperitoneal injection of diabetic streptozotocin and were randomly divided into three groups: endurance training, resistance training and control group. The rats of the endurance training group were trained on the treadmill for 8 weeks, 5 days a week with intensity of vo2Max 60-80%. The resistance training group was trained on the ladder with a slope of 85 degrees and with a weight equals to 30-100% of their body weight. Forty eight hours after the last training session, blood samples were collected and ventricular tissues of mice were extracted. Glucose, insulin, serum insulin resistance index and Bcl-2, Bax, caspase 8 gene expression levels and Bax to Bcl-2 ratio were evaluated. Masson's trichrome and hematoxylin-eosin staining methods were used for histological examination of diabetic rat's heart structure to detect fibrosis.
Results: There was a significant decrease in Bax gene expression and the ratio of Bax to Bcl-2, and also there was a significant increase in Bcl-2 and caspase 8 in the endurance and resistance training groups in comparison with the control group. The rate of cardiomyocyte fiber rupture in the endurance and resistance groups was less than the control group, and the presence of lymphocyte cells was observed in some fibers in the control group. (p≤0.05).
Conclusion: The results showed that high-intensity resistance training and moderate-intensity endurance training can prevent tissue fibrosis caused by collagen deposition in diabetes, and these two types of training can reduce the factors involved in apoptosis both in the internal and external pathways. On the other hand, this training intensity can be used as an effective non-pharmacological method to reduce the problems of apoptosis and fibrosis caused by diabetes in the heart tissue. |
| first_indexed | 2024-04-10T22:23:48Z |
| format | Article |
| id | doaj.art-1b1c980138b84c45b391d5d65653a8ff |
| institution | Directory Open Access Journal |
| issn | 2228-7280 2228-7299 |
| language | fas |
| last_indexed | 2024-04-10T22:23:48Z |
| publishDate | 2022-07-01 |
| publisher | Ardabil University of Medical Sciences |
| record_format | Article |
| series | Journal of Ardabil University of Medical Sciences |
| spelling | doaj.art-1b1c980138b84c45b391d5d65653a8ff2023-01-17T09:42:13ZfasArdabil University of Medical SciencesJournal of Ardabil University of Medical Sciences2228-72802228-72992022-07-01222186203The Effect of Endurance and Resistance Training on Apoptosis Activity and Collagen Deposition in Heart Tissue of Diabetic RatsHamideh Montazery Taleghani0Nader Shakeri1Khosro Ebrahim2Rahman Soori3Mandana Gholami4 Department of Sports Physiology, Cardiovascular and Respiratory, Faculty of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran Department of Sports Physiology, Faculty of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran Department of Sports Physiology, Cardiovascular, Faculty of Physical Education and Sports Sciences, Shahid Beheshty University, Tehran, Iran Department of Sports Physiology, Faculty of Physical Education and Sports Sciences, Tehran University, Tehran, Iran Department of Sports Physiology, Faculty of Physical Education and Sports Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran Background & objective: Diabetes causes chronic problems in the structure and function of tissues, such as apoptosis and fibrosis in addition to glycemic disorders. In this study the effect of 8 weeks of endurance and resistance training on various signaling pathways of apoptosis and tissue fibrosis of the heart of diabetic rats was investigated. Methods: Thirty Wistar rats, approximately 8-10 weeks old, weight about 210-250 grams, received intraperitoneal injection of diabetic streptozotocin and were randomly divided into three groups: endurance training, resistance training and control group. The rats of the endurance training group were trained on the treadmill for 8 weeks, 5 days a week with intensity of vo2Max 60-80%. The resistance training group was trained on the ladder with a slope of 85 degrees and with a weight equals to 30-100% of their body weight. Forty eight hours after the last training session, blood samples were collected and ventricular tissues of mice were extracted. Glucose, insulin, serum insulin resistance index and Bcl-2, Bax, caspase 8 gene expression levels and Bax to Bcl-2 ratio were evaluated. Masson's trichrome and hematoxylin-eosin staining methods were used for histological examination of diabetic rat's heart structure to detect fibrosis. Results: There was a significant decrease in Bax gene expression and the ratio of Bax to Bcl-2, and also there was a significant increase in Bcl-2 and caspase 8 in the endurance and resistance training groups in comparison with the control group. The rate of cardiomyocyte fiber rupture in the endurance and resistance groups was less than the control group, and the presence of lymphocyte cells was observed in some fibers in the control group. (p≤0.05). Conclusion: The results showed that high-intensity resistance training and moderate-intensity endurance training can prevent tissue fibrosis caused by collagen deposition in diabetes, and these two types of training can reduce the factors involved in apoptosis both in the internal and external pathways. On the other hand, this training intensity can be used as an effective non-pharmacological method to reduce the problems of apoptosis and fibrosis caused by diabetes in the heart tissue.http://jarums.arums.ac.ir/article-1-2194-en.htmlendurance trainingresistance trainingtype 2 diabetesapoptosisfibrosis |
| spellingShingle | Hamideh Montazery Taleghani Nader Shakeri Khosro Ebrahim Rahman Soori Mandana Gholami The Effect of Endurance and Resistance Training on Apoptosis Activity and Collagen Deposition in Heart Tissue of Diabetic Rats Journal of Ardabil University of Medical Sciences endurance training resistance training type 2 diabetes apoptosis fibrosis |
| title | The Effect of Endurance and Resistance Training on Apoptosis Activity and Collagen Deposition in Heart Tissue of Diabetic Rats |
| title_full | The Effect of Endurance and Resistance Training on Apoptosis Activity and Collagen Deposition in Heart Tissue of Diabetic Rats |
| title_fullStr | The Effect of Endurance and Resistance Training on Apoptosis Activity and Collagen Deposition in Heart Tissue of Diabetic Rats |
| title_full_unstemmed | The Effect of Endurance and Resistance Training on Apoptosis Activity and Collagen Deposition in Heart Tissue of Diabetic Rats |
| title_short | The Effect of Endurance and Resistance Training on Apoptosis Activity and Collagen Deposition in Heart Tissue of Diabetic Rats |
| title_sort | effect of endurance and resistance training on apoptosis activity and collagen deposition in heart tissue of diabetic rats |
| topic | endurance training resistance training type 2 diabetes apoptosis fibrosis |
| url | http://jarums.arums.ac.ir/article-1-2194-en.html |
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