Novel Gel Formulations as Topical Carriers for the Essential Oil of Bidens tripartita for the Treatment of Candidiasis
The genus Bidens L. (Asteraceae) refers to several species of plants used in traditional phytotherapeutic preparations. B. tripartita, also known as bur marigold, is the most familiar plant and has been known as a remedy for chronic dysentery. The hydrodistilled essential oil of the aerial parts of...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-10-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/23/10/2517 |
| _version_ | 1819107724446662656 |
|---|---|
| author | Monika Tomczykowa Magdalena Wróblewska Katarzyna Winnicka Piotr Wieczorek Piotr Majewski Katarzyna Celińska-Janowicz Róża Sawczuk Wojciech Miltyk Elżbieta Tryniszewska Michał Tomczyk |
| author_facet | Monika Tomczykowa Magdalena Wróblewska Katarzyna Winnicka Piotr Wieczorek Piotr Majewski Katarzyna Celińska-Janowicz Róża Sawczuk Wojciech Miltyk Elżbieta Tryniszewska Michał Tomczyk |
| author_sort | Monika Tomczykowa |
| collection | DOAJ |
| description | The genus Bidens L. (Asteraceae) refers to several species of plants used in traditional phytotherapeutic preparations. B. tripartita, also known as bur marigold, is the most familiar plant and has been known as a remedy for chronic dysentery. The hydrodistilled essential oil of the aerial parts of the Polish B. tripartita was analyzed using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) techniques. To exclude any potential toxic effects of the oil on human dermal fibroblasts, the MTT test (methyl thiazolyl tetrazolium) and COMET assay (single-cell gel electrophoresis) were performed. Novel gel formulations as topical carriers for essential oil obtained from B. tripartita were developed and characterized. The bioadhesive properties of the designed preparations in the ex vivo model using the skin of hairless mice were also evaluated. The therapeutic efficacy of the topical formulations is influenced by active phytoconstituents and vehicle characteristics. The antifungal properties of the essential oil of B. tripartita were also tested against Candida species, and this oil appears to be a promising topical anticandidal agent. |
| first_indexed | 2024-12-22T02:58:35Z |
| format | Article |
| id | doaj.art-1b25dd2a9e034f4787f3ea4b1f8126d3 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T02:58:35Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1b25dd2a9e034f4787f3ea4b1f8126d32022-12-21T18:41:12ZengMDPI AGMolecules1420-30492018-10-012310251710.3390/molecules23102517molecules23102517Novel Gel Formulations as Topical Carriers for the Essential Oil of Bidens tripartita for the Treatment of CandidiasisMonika Tomczykowa0Magdalena Wróblewska1Katarzyna Winnicka2Piotr Wieczorek3Piotr Majewski4Katarzyna Celińska-Janowicz5Róża Sawczuk6Wojciech Miltyk7Elżbieta Tryniszewska8Michał Tomczyk9Department of Organic Chemistry, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, 15-230 Białystok, PolandDepartment of Pharmaceutical Technology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, 15-222 Białystok, PolandDepartment of Pharmaceutical Technology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, 15-222 Białystok, PolandDepartment of Microbiological Diagnostics and Infectious Immunology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, 15-269 Białystok, PolandDepartment of Microbiological Diagnostics and Infectious Immunology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, 15-269 Białystok, PolandDepartment of Pharmaceutical Analysis, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, 15-222 Białystok, PolandDepartment of Pharmaceutical Analysis, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, 15-222 Białystok, PolandDepartment of Pharmaceutical Analysis, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, 15-222 Białystok, PolandDepartment of Microbiological Diagnostics and Infectious Immunology, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, 15-269 Białystok, PolandDepartment of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Białystok, 15-230 Białystok, PolandThe genus Bidens L. (Asteraceae) refers to several species of plants used in traditional phytotherapeutic preparations. B. tripartita, also known as bur marigold, is the most familiar plant and has been known as a remedy for chronic dysentery. The hydrodistilled essential oil of the aerial parts of the Polish B. tripartita was analyzed using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) techniques. To exclude any potential toxic effects of the oil on human dermal fibroblasts, the MTT test (methyl thiazolyl tetrazolium) and COMET assay (single-cell gel electrophoresis) were performed. Novel gel formulations as topical carriers for essential oil obtained from B. tripartita were developed and characterized. The bioadhesive properties of the designed preparations in the ex vivo model using the skin of hairless mice were also evaluated. The therapeutic efficacy of the topical formulations is influenced by active phytoconstituents and vehicle characteristics. The antifungal properties of the essential oil of B. tripartita were also tested against Candida species, and this oil appears to be a promising topical anticandidal agent.http://www.mdpi.com/1420-3049/23/10/2517Bidens tripartitaessential oiltopical formulationantifungal activitygenotoxicity |
| spellingShingle | Monika Tomczykowa Magdalena Wróblewska Katarzyna Winnicka Piotr Wieczorek Piotr Majewski Katarzyna Celińska-Janowicz Róża Sawczuk Wojciech Miltyk Elżbieta Tryniszewska Michał Tomczyk Novel Gel Formulations as Topical Carriers for the Essential Oil of Bidens tripartita for the Treatment of Candidiasis Molecules Bidens tripartita essential oil topical formulation antifungal activity genotoxicity |
| title | Novel Gel Formulations as Topical Carriers for the Essential Oil of Bidens tripartita for the Treatment of Candidiasis |
| title_full | Novel Gel Formulations as Topical Carriers for the Essential Oil of Bidens tripartita for the Treatment of Candidiasis |
| title_fullStr | Novel Gel Formulations as Topical Carriers for the Essential Oil of Bidens tripartita for the Treatment of Candidiasis |
| title_full_unstemmed | Novel Gel Formulations as Topical Carriers for the Essential Oil of Bidens tripartita for the Treatment of Candidiasis |
| title_short | Novel Gel Formulations as Topical Carriers for the Essential Oil of Bidens tripartita for the Treatment of Candidiasis |
| title_sort | novel gel formulations as topical carriers for the essential oil of bidens tripartita for the treatment of candidiasis |
| topic | Bidens tripartita essential oil topical formulation antifungal activity genotoxicity |
| url | http://www.mdpi.com/1420-3049/23/10/2517 |
| work_keys_str_mv | AT monikatomczykowa novelgelformulationsastopicalcarriersfortheessentialoilofbidenstripartitaforthetreatmentofcandidiasis AT magdalenawroblewska novelgelformulationsastopicalcarriersfortheessentialoilofbidenstripartitaforthetreatmentofcandidiasis AT katarzynawinnicka novelgelformulationsastopicalcarriersfortheessentialoilofbidenstripartitaforthetreatmentofcandidiasis AT piotrwieczorek novelgelformulationsastopicalcarriersfortheessentialoilofbidenstripartitaforthetreatmentofcandidiasis AT piotrmajewski novelgelformulationsastopicalcarriersfortheessentialoilofbidenstripartitaforthetreatmentofcandidiasis AT katarzynacelinskajanowicz novelgelformulationsastopicalcarriersfortheessentialoilofbidenstripartitaforthetreatmentofcandidiasis AT rozasawczuk novelgelformulationsastopicalcarriersfortheessentialoilofbidenstripartitaforthetreatmentofcandidiasis AT wojciechmiltyk novelgelformulationsastopicalcarriersfortheessentialoilofbidenstripartitaforthetreatmentofcandidiasis AT elzbietatryniszewska novelgelformulationsastopicalcarriersfortheessentialoilofbidenstripartitaforthetreatmentofcandidiasis AT michałtomczyk novelgelformulationsastopicalcarriersfortheessentialoilofbidenstripartitaforthetreatmentofcandidiasis |