Acriflavine: an efficient green fluorescent probe for sensitive analysis of aceclofenac in pharmaceutical formulations
Abstract Acriflavine is a multipurpose drug that shows antibacterial, antiviral, antimalarial, and antifungal activities. The remarkable native fluorescence of acriflavine is exploited in analytical chemistry field as an efficient probe for analysis of pharmaceutical and biological compounds. The fl...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | BMC Chemistry |
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| Online Access: | https://doi.org/10.1186/s13065-023-00979-2 |
| _version_ | 1797363641234751488 |
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| author | Amal A. El-Masry Abdallah M. Zeid |
| author_facet | Amal A. El-Masry Abdallah M. Zeid |
| author_sort | Amal A. El-Masry |
| collection | DOAJ |
| description | Abstract Acriflavine is a multipurpose drug that shows antibacterial, antiviral, antimalarial, and antifungal activities. The remarkable native fluorescence of acriflavine is exploited in analytical chemistry field as an efficient probe for analysis of pharmaceutical and biological compounds. The fluorescent probe action of acriflavine is based on the remarkable fluorescence turning-off via formation of ion-pair complexes with acidic drugs at a specific pH. Herein, the acidic drug aceclofenac is analysed for the first time using acriflavine as a fluorescent probe. Aceclofenac can form an ion-pair complex with acriflavine at pH 8.5, and hence it partially turns off the fluorescence intensity of acriflavine over a concentration range of 1–20 µg/mL. The fluorescence quenching was monitored at 502 nm following an excitation at 265 or 451 nm. The reaction stoichiometry between acriflavine and aceclofenac was found to be 1:1 using limiting logarithmic method. The type of quenching was confirmed to be static using Stern–Volmer plot. The method showed low values of quantitation limit (0.89 µg/mL) and detection limit (0.29 µg/mL). Moreover, the method was linear (r = 0.9999), accurate, precise (RSD < 1.7%), robust, and specific. The proposed method was successfully employed to analyse aceclofenac in its dosage forms with high %recovery (98–101%). Additionally, GAPI and AGREE approaches were used to guarantee the suggested techniques' greenness, and the findings showed an excellent level of greenness. |
| first_indexed | 2024-03-08T16:24:00Z |
| format | Article |
| id | doaj.art-1b279b40a91c48479d317beb333e1ba3 |
| institution | Directory Open Access Journal |
| issn | 2661-801X |
| language | English |
| last_indexed | 2024-03-08T16:24:00Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Chemistry |
| spelling | doaj.art-1b279b40a91c48479d317beb333e1ba32024-01-07T12:06:55ZengBMCBMC Chemistry2661-801X2023-08-0117111310.1186/s13065-023-00979-2Acriflavine: an efficient green fluorescent probe for sensitive analysis of aceclofenac in pharmaceutical formulationsAmal A. El-Masry0Abdallah M. Zeid1Department of Medicinal Chemistry, Faculty of Pharmacy, Mansoura UniversityDepartment of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura UniversityAbstract Acriflavine is a multipurpose drug that shows antibacterial, antiviral, antimalarial, and antifungal activities. The remarkable native fluorescence of acriflavine is exploited in analytical chemistry field as an efficient probe for analysis of pharmaceutical and biological compounds. The fluorescent probe action of acriflavine is based on the remarkable fluorescence turning-off via formation of ion-pair complexes with acidic drugs at a specific pH. Herein, the acidic drug aceclofenac is analysed for the first time using acriflavine as a fluorescent probe. Aceclofenac can form an ion-pair complex with acriflavine at pH 8.5, and hence it partially turns off the fluorescence intensity of acriflavine over a concentration range of 1–20 µg/mL. The fluorescence quenching was monitored at 502 nm following an excitation at 265 or 451 nm. The reaction stoichiometry between acriflavine and aceclofenac was found to be 1:1 using limiting logarithmic method. The type of quenching was confirmed to be static using Stern–Volmer plot. The method showed low values of quantitation limit (0.89 µg/mL) and detection limit (0.29 µg/mL). Moreover, the method was linear (r = 0.9999), accurate, precise (RSD < 1.7%), robust, and specific. The proposed method was successfully employed to analyse aceclofenac in its dosage forms with high %recovery (98–101%). Additionally, GAPI and AGREE approaches were used to guarantee the suggested techniques' greenness, and the findings showed an excellent level of greenness.https://doi.org/10.1186/s13065-023-00979-2AcriflavineAceclofenacFluorescent probeStern–Volmer plotDosage form |
| spellingShingle | Amal A. El-Masry Abdallah M. Zeid Acriflavine: an efficient green fluorescent probe for sensitive analysis of aceclofenac in pharmaceutical formulations BMC Chemistry Acriflavine Aceclofenac Fluorescent probe Stern–Volmer plot Dosage form |
| title | Acriflavine: an efficient green fluorescent probe for sensitive analysis of aceclofenac in pharmaceutical formulations |
| title_full | Acriflavine: an efficient green fluorescent probe for sensitive analysis of aceclofenac in pharmaceutical formulations |
| title_fullStr | Acriflavine: an efficient green fluorescent probe for sensitive analysis of aceclofenac in pharmaceutical formulations |
| title_full_unstemmed | Acriflavine: an efficient green fluorescent probe for sensitive analysis of aceclofenac in pharmaceutical formulations |
| title_short | Acriflavine: an efficient green fluorescent probe for sensitive analysis of aceclofenac in pharmaceutical formulations |
| title_sort | acriflavine an efficient green fluorescent probe for sensitive analysis of aceclofenac in pharmaceutical formulations |
| topic | Acriflavine Aceclofenac Fluorescent probe Stern–Volmer plot Dosage form |
| url | https://doi.org/10.1186/s13065-023-00979-2 |
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