Effect of Inoculation with Arbuscular Mycorrhizal Fungi and Fungicide Application on the Secondary Metabolism of <i>Solanum tuberosum</i> Leaves
In potato (<i>Solanum tuberosum</i>) crops, the use of fungicides to control some diseases is widespread; however, it has been reported that this practice can modify the potato polyphenolic content, and new strategies oriented to the potato defense system are necessary. One alternative i...
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MDPI AG
2022-01-01
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| Series: | Plants |
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| author | Valentina Fritz Gonzalo Tereucán Christian Santander Boris Contreras Pablo Cornejo Paulo Ademar Avelar Ferreira Antonieta Ruiz |
| author_facet | Valentina Fritz Gonzalo Tereucán Christian Santander Boris Contreras Pablo Cornejo Paulo Ademar Avelar Ferreira Antonieta Ruiz |
| author_sort | Valentina Fritz |
| collection | DOAJ |
| description | In potato (<i>Solanum tuberosum</i>) crops, the use of fungicides to control some diseases is widespread; however, it has been reported that this practice can modify the potato polyphenolic content, and new strategies oriented to the potato defense system are necessary. One alternative is the use of arbuscular mycorrhizal fungi (AMF) to improve the defense mechanisms of plants. In this study, phenolic profiles and antioxidant activities in leaves of three potato genotypes (CB2011-509, CB2011-104, and VR808) were evaluated in crops inoculated with three AMF strains (<i>Claroideoglomus claroideum, Claroideoglomus lamellosum,</i> and <i>Fumneliformis mosseae</i>) and with AMF in combination with the use of two commercial fungicides (MONCUT [M] and ReflectXtra [R]). Eight phenolic compounds were detected, mainly hydroxycinnamic acids (HCAD) and flavonols, in samples where the highest concentrations of HCAD were obtained, 5-caffeoylquinic acid was the most abundant phenolic. The antioxidant activity was higher using the cupric reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP) methods. The association of AMF with plants had benefits on the secondary metabolism; however, the response differed according to genotype. The different combinations of potato genotypes, AMF strain, and fungicide modified the content of phenolic compounds in leaves in different ways; the treatment using <i>C. lamellosum</i> and ReflectXtra was the ideal combination for the genotypes analyzed here, with the higher antioxidant response, which supports the further technological evaluation of efficient AMF strains and fungicides in potato crops. |
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| spelling | doaj.art-1b28f00e9799479ea836749af27c7d322023-11-23T17:29:18ZengMDPI AGPlants2223-77472022-01-0111327810.3390/plants11030278Effect of Inoculation with Arbuscular Mycorrhizal Fungi and Fungicide Application on the Secondary Metabolism of <i>Solanum tuberosum</i> LeavesValentina Fritz0Gonzalo Tereucán1Christian Santander2Boris Contreras3Pablo Cornejo4Paulo Ademar Avelar Ferreira5Antonieta Ruiz6Centro de Investigación en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Universidad de La Frontera, Avda. Francisco Salazar 01145, Temuco 4811230, ChileCentro de Investigación en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Universidad de La Frontera, Avda. Francisco Salazar 01145, Temuco 4811230, ChileCentro de Investigación en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Universidad de La Frontera, Avda. Francisco Salazar 01145, Temuco 4811230, ChileNovaseed Ltda. and Papas Arcoiris Ltda., Loteo Pozo de Ripio s/n, Parque Ivian II, Puerto Varas 5550000, ChileCentro de Investigación en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Universidad de La Frontera, Avda. Francisco Salazar 01145, Temuco 4811230, ChileDepartamento de Solos, Universidade Federal de Santa Maria, Santa Maria 97105-900, RS, BrazilCentro de Investigación en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Universidad de La Frontera, Avda. Francisco Salazar 01145, Temuco 4811230, ChileIn potato (<i>Solanum tuberosum</i>) crops, the use of fungicides to control some diseases is widespread; however, it has been reported that this practice can modify the potato polyphenolic content, and new strategies oriented to the potato defense system are necessary. One alternative is the use of arbuscular mycorrhizal fungi (AMF) to improve the defense mechanisms of plants. In this study, phenolic profiles and antioxidant activities in leaves of three potato genotypes (CB2011-509, CB2011-104, and VR808) were evaluated in crops inoculated with three AMF strains (<i>Claroideoglomus claroideum, Claroideoglomus lamellosum,</i> and <i>Fumneliformis mosseae</i>) and with AMF in combination with the use of two commercial fungicides (MONCUT [M] and ReflectXtra [R]). Eight phenolic compounds were detected, mainly hydroxycinnamic acids (HCAD) and flavonols, in samples where the highest concentrations of HCAD were obtained, 5-caffeoylquinic acid was the most abundant phenolic. The antioxidant activity was higher using the cupric reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP) methods. The association of AMF with plants had benefits on the secondary metabolism; however, the response differed according to genotype. The different combinations of potato genotypes, AMF strain, and fungicide modified the content of phenolic compounds in leaves in different ways; the treatment using <i>C. lamellosum</i> and ReflectXtra was the ideal combination for the genotypes analyzed here, with the higher antioxidant response, which supports the further technological evaluation of efficient AMF strains and fungicides in potato crops.https://www.mdpi.com/2223-7747/11/3/278potatomycorrhizasecondary metabolismantioxidant activitiesphenolic compounds |
| spellingShingle | Valentina Fritz Gonzalo Tereucán Christian Santander Boris Contreras Pablo Cornejo Paulo Ademar Avelar Ferreira Antonieta Ruiz Effect of Inoculation with Arbuscular Mycorrhizal Fungi and Fungicide Application on the Secondary Metabolism of <i>Solanum tuberosum</i> Leaves Plants potato mycorrhiza secondary metabolism antioxidant activities phenolic compounds |
| title | Effect of Inoculation with Arbuscular Mycorrhizal Fungi and Fungicide Application on the Secondary Metabolism of <i>Solanum tuberosum</i> Leaves |
| title_full | Effect of Inoculation with Arbuscular Mycorrhizal Fungi and Fungicide Application on the Secondary Metabolism of <i>Solanum tuberosum</i> Leaves |
| title_fullStr | Effect of Inoculation with Arbuscular Mycorrhizal Fungi and Fungicide Application on the Secondary Metabolism of <i>Solanum tuberosum</i> Leaves |
| title_full_unstemmed | Effect of Inoculation with Arbuscular Mycorrhizal Fungi and Fungicide Application on the Secondary Metabolism of <i>Solanum tuberosum</i> Leaves |
| title_short | Effect of Inoculation with Arbuscular Mycorrhizal Fungi and Fungicide Application on the Secondary Metabolism of <i>Solanum tuberosum</i> Leaves |
| title_sort | effect of inoculation with arbuscular mycorrhizal fungi and fungicide application on the secondary metabolism of i solanum tuberosum i leaves |
| topic | potato mycorrhiza secondary metabolism antioxidant activities phenolic compounds |
| url | https://www.mdpi.com/2223-7747/11/3/278 |
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