Plant Production Protocols from Seeds of Threatened <i>Atropa baetica</i> and Widespread <i>A. belladonna</i>, Both Rich in Alkaloids
Members of the genus <i>Atropa</i> contain various tropane alkaloids, including atropine ((±)-hyoscyamine) and scopolamine, which possess medicinal properties. Preserving the diverse genetic background of wild populations via optimal plant production from seeds could be essential for avo...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/13/11/2181 |
| _version_ | 1797458624621051904 |
|---|---|
| author | Elena Copete Miguel A. Copete Esmeralda Martínez-Duro Alejandro Santiago Pablo Ferrandis José M. Herranz |
| author_facet | Elena Copete Miguel A. Copete Esmeralda Martínez-Duro Alejandro Santiago Pablo Ferrandis José M. Herranz |
| author_sort | Elena Copete |
| collection | DOAJ |
| description | Members of the genus <i>Atropa</i> contain various tropane alkaloids, including atropine ((±)-hyoscyamine) and scopolamine, which possess medicinal properties. Preserving the diverse genetic background of wild populations via optimal plant production from seeds could be essential for avoiding the loss of potential uses. We analyzed the germination ecology of two <i>Atropa</i> species comprising the threatened <i>A. baetica</i> and widespread <i>A. belladonna</i> to determine the: (1) influence of temperature, light, and seed age on germination patterns; (2) effects of cold stratification and gibberellic acid (GA<sub>3</sub>); (3) phenology of seedling emergence in outdoor conditions; (4) phenology of dormancy break and loss of viability in buried seeds; and (5) ability to form persistent soil seed banks. Freshly matured seeds exhibited conditional physiological dormancy, with germination at high temperatures (32/18 °C) but not at low and cold ones (5, 15/4, 20/7 °C). The germination ability increased with time of dry storage and with GA<sub>3</sub>, thereby suggesting nondeep physiological dormancy. Under outdoor conditions, no seedlings emerged during the first post-sown autumn, but emergence peaks occurred in late winter–early spring. Both species could form small persistent soil seed banks with short durations (3–5 years). A plant production protocol from seeds was established for both taxa. |
| first_indexed | 2024-03-09T16:39:49Z |
| format | Article |
| id | doaj.art-1b2af469bba94e1ab60ecaea78df9de3 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T16:39:49Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-1b2af469bba94e1ab60ecaea78df9de32023-11-24T14:52:29ZengMDPI AGLife2075-17292023-11-011311218110.3390/life13112181Plant Production Protocols from Seeds of Threatened <i>Atropa baetica</i> and Widespread <i>A. belladonna</i>, Both Rich in AlkaloidsElena Copete0Miguel A. Copete1Esmeralda Martínez-Duro2Alejandro Santiago3Pablo Ferrandis4José M. Herranz5ETSIAMB, Department of Plant Production and Agricultural Technology, University of Castilla-La Mancha, University Campus s/n, 02071 Albacete, SpainETSIAMB, Department of Plant Production and Agricultural Technology, University of Castilla-La Mancha, University Campus s/n, 02071 Albacete, SpainETSIAMB, Department of Plant Production and Agricultural Technology, University of Castilla-La Mancha, University Campus s/n, 02071 Albacete, SpainBotanical Institute, University of Castilla-La Mancha, Avenida de la Mancha s/n, 02006 Albacete, SpainETSIAMB, Department of Plant Production and Agricultural Technology, University of Castilla-La Mancha, University Campus s/n, 02071 Albacete, SpainETSIAMB, Department of Plant Production and Agricultural Technology, University of Castilla-La Mancha, University Campus s/n, 02071 Albacete, SpainMembers of the genus <i>Atropa</i> contain various tropane alkaloids, including atropine ((±)-hyoscyamine) and scopolamine, which possess medicinal properties. Preserving the diverse genetic background of wild populations via optimal plant production from seeds could be essential for avoiding the loss of potential uses. We analyzed the germination ecology of two <i>Atropa</i> species comprising the threatened <i>A. baetica</i> and widespread <i>A. belladonna</i> to determine the: (1) influence of temperature, light, and seed age on germination patterns; (2) effects of cold stratification and gibberellic acid (GA<sub>3</sub>); (3) phenology of seedling emergence in outdoor conditions; (4) phenology of dormancy break and loss of viability in buried seeds; and (5) ability to form persistent soil seed banks. Freshly matured seeds exhibited conditional physiological dormancy, with germination at high temperatures (32/18 °C) but not at low and cold ones (5, 15/4, 20/7 °C). The germination ability increased with time of dry storage and with GA<sub>3</sub>, thereby suggesting nondeep physiological dormancy. Under outdoor conditions, no seedlings emerged during the first post-sown autumn, but emergence peaks occurred in late winter–early spring. Both species could form small persistent soil seed banks with short durations (3–5 years). A plant production protocol from seeds was established for both taxa.https://www.mdpi.com/2075-1729/13/11/2181<i>Atropa baetica</i> Willk.<i>Atropa belladonna</i> L.dormancy breakagegerminationnondeep physiological dormancySolanaceae |
| spellingShingle | Elena Copete Miguel A. Copete Esmeralda Martínez-Duro Alejandro Santiago Pablo Ferrandis José M. Herranz Plant Production Protocols from Seeds of Threatened <i>Atropa baetica</i> and Widespread <i>A. belladonna</i>, Both Rich in Alkaloids Life <i>Atropa baetica</i> Willk. <i>Atropa belladonna</i> L. dormancy breakage germination nondeep physiological dormancy Solanaceae |
| title | Plant Production Protocols from Seeds of Threatened <i>Atropa baetica</i> and Widespread <i>A. belladonna</i>, Both Rich in Alkaloids |
| title_full | Plant Production Protocols from Seeds of Threatened <i>Atropa baetica</i> and Widespread <i>A. belladonna</i>, Both Rich in Alkaloids |
| title_fullStr | Plant Production Protocols from Seeds of Threatened <i>Atropa baetica</i> and Widespread <i>A. belladonna</i>, Both Rich in Alkaloids |
| title_full_unstemmed | Plant Production Protocols from Seeds of Threatened <i>Atropa baetica</i> and Widespread <i>A. belladonna</i>, Both Rich in Alkaloids |
| title_short | Plant Production Protocols from Seeds of Threatened <i>Atropa baetica</i> and Widespread <i>A. belladonna</i>, Both Rich in Alkaloids |
| title_sort | plant production protocols from seeds of threatened i atropa baetica i and widespread i a belladonna i both rich in alkaloids |
| topic | <i>Atropa baetica</i> Willk. <i>Atropa belladonna</i> L. dormancy breakage germination nondeep physiological dormancy Solanaceae |
| url | https://www.mdpi.com/2075-1729/13/11/2181 |
| work_keys_str_mv | AT elenacopete plantproductionprotocolsfromseedsofthreatenediatropabaeticaiandwidespreadiabelladonnaibothrichinalkaloids AT miguelacopete plantproductionprotocolsfromseedsofthreatenediatropabaeticaiandwidespreadiabelladonnaibothrichinalkaloids AT esmeraldamartinezduro plantproductionprotocolsfromseedsofthreatenediatropabaeticaiandwidespreadiabelladonnaibothrichinalkaloids AT alejandrosantiago plantproductionprotocolsfromseedsofthreatenediatropabaeticaiandwidespreadiabelladonnaibothrichinalkaloids AT pabloferrandis plantproductionprotocolsfromseedsofthreatenediatropabaeticaiandwidespreadiabelladonnaibothrichinalkaloids AT josemherranz plantproductionprotocolsfromseedsofthreatenediatropabaeticaiandwidespreadiabelladonnaibothrichinalkaloids |