Pathological and Molecular Characterization of a Duck Plague Outbreak in Southern China in 2021
Duck plague (DP) is a highly contagious viral disease in ducks caused by the duck plague virus (DPV). The DPV, a member of <i>Herpesviridae</i>, poses a severe threat to the waterfowl farming industry worldwide. In this study, we reported a recent outbreak of DPV in domestic laying ducks...
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MDPI AG
2022-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/24/3523 |
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| author | Zhipeng Liang Jinyue Guo Sheng Yuan Qing Cheng Xinyu Zhang Zhun Liu Congying Wang Zhili Li Bo Hou Shujian Huang Feng Wen |
| author_facet | Zhipeng Liang Jinyue Guo Sheng Yuan Qing Cheng Xinyu Zhang Zhun Liu Congying Wang Zhili Li Bo Hou Shujian Huang Feng Wen |
| author_sort | Zhipeng Liang |
| collection | DOAJ |
| description | Duck plague (DP) is a highly contagious viral disease in ducks caused by the duck plague virus (DPV). The DPV, a member of <i>Herpesviridae</i>, poses a severe threat to the waterfowl farming industry worldwide. In this study, we reported a recent outbreak of DPV in domestic laying ducks at 310 days of age from southern China in December 2021. The gross lesion, histopathologic examination, molecular detection, and genetic characterization studies of DPV are described here. As a result, gross lesions such as an enlarged congestive spleen and liver were observed. Liver with vacuolar degeneration and small vacuoles and spleen with hemosiderosis were remarkable microscopic findings. Our results suggested that the liver had the highest viral load, followed by the trachea, pancreas, kidney, brain, spleen, and heart. In addition, DPV was successfully isolated in chicken embryo fibroblast cell culture and designated as DP-GD-305-21. The <i>UL2</i>, <i>UL12</i>, <i>UL41</i>, <i>UL47</i>, and <i>LORF11</i> genes of DP-GD-305-21 shared a high nucleotide homology with the Chinese virulent (CHv) strain and the Chinese variant (CV) strain. In conclusion, this study reports the isolation and molecular characterization of DPV from a recent outbreak in southern China. Our results contributed to the understanding of the pathological and molecular characterization of currently circulating DPV in China. |
| first_indexed | 2024-03-09T17:24:42Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T17:24:42Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-1b2cc3b2ed904f46a1b0a54dca8a9d1a2023-11-24T12:51:13ZengMDPI AGAnimals2076-26152022-12-011224352310.3390/ani12243523Pathological and Molecular Characterization of a Duck Plague Outbreak in Southern China in 2021Zhipeng Liang0Jinyue Guo1Sheng Yuan2Qing Cheng3Xinyu Zhang4Zhun Liu5Congying Wang6Zhili Li7Bo Hou8Shujian Huang9Feng Wen10College of Life Science and Engineering, Foshan University, Foshan 528231, ChinaCollege of Life Science and Engineering, Foshan University, Foshan 528231, ChinaCollege of Life Science and Engineering, Foshan University, Foshan 528231, ChinaCollege of Life Science and Engineering, Foshan University, Foshan 528231, ChinaCollege of Life Science and Engineering, Foshan University, Foshan 528231, ChinaCollege of Life Science and Engineering, Foshan University, Foshan 528231, ChinaCollege of Life Science and Engineering, Foshan University, Foshan 528231, ChinaCollege of Life Science and Engineering, Foshan University, Foshan 528231, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences/Fujian Animal Disease Control Technology Development Center, Fuzhou 350013, ChinaCollege of Life Science and Engineering, Foshan University, Foshan 528231, ChinaCollege of Life Science and Engineering, Foshan University, Foshan 528231, ChinaDuck plague (DP) is a highly contagious viral disease in ducks caused by the duck plague virus (DPV). The DPV, a member of <i>Herpesviridae</i>, poses a severe threat to the waterfowl farming industry worldwide. In this study, we reported a recent outbreak of DPV in domestic laying ducks at 310 days of age from southern China in December 2021. The gross lesion, histopathologic examination, molecular detection, and genetic characterization studies of DPV are described here. As a result, gross lesions such as an enlarged congestive spleen and liver were observed. Liver with vacuolar degeneration and small vacuoles and spleen with hemosiderosis were remarkable microscopic findings. Our results suggested that the liver had the highest viral load, followed by the trachea, pancreas, kidney, brain, spleen, and heart. In addition, DPV was successfully isolated in chicken embryo fibroblast cell culture and designated as DP-GD-305-21. The <i>UL2</i>, <i>UL12</i>, <i>UL41</i>, <i>UL47</i>, and <i>LORF11</i> genes of DP-GD-305-21 shared a high nucleotide homology with the Chinese virulent (CHv) strain and the Chinese variant (CV) strain. In conclusion, this study reports the isolation and molecular characterization of DPV from a recent outbreak in southern China. Our results contributed to the understanding of the pathological and molecular characterization of currently circulating DPV in China.https://www.mdpi.com/2076-2615/12/24/3523duck plagueduck plague virus<i>Herpesviridae</i><i>UL2</i><i>LORF11</i> |
| spellingShingle | Zhipeng Liang Jinyue Guo Sheng Yuan Qing Cheng Xinyu Zhang Zhun Liu Congying Wang Zhili Li Bo Hou Shujian Huang Feng Wen Pathological and Molecular Characterization of a Duck Plague Outbreak in Southern China in 2021 Animals duck plague duck plague virus <i>Herpesviridae</i> <i>UL2</i> <i>LORF11</i> |
| title | Pathological and Molecular Characterization of a Duck Plague Outbreak in Southern China in 2021 |
| title_full | Pathological and Molecular Characterization of a Duck Plague Outbreak in Southern China in 2021 |
| title_fullStr | Pathological and Molecular Characterization of a Duck Plague Outbreak in Southern China in 2021 |
| title_full_unstemmed | Pathological and Molecular Characterization of a Duck Plague Outbreak in Southern China in 2021 |
| title_short | Pathological and Molecular Characterization of a Duck Plague Outbreak in Southern China in 2021 |
| title_sort | pathological and molecular characterization of a duck plague outbreak in southern china in 2021 |
| topic | duck plague duck plague virus <i>Herpesviridae</i> <i>UL2</i> <i>LORF11</i> |
| url | https://www.mdpi.com/2076-2615/12/24/3523 |
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