<i>OsGGC2</i>, Gγ Subunit of Heterotrimeric G Protein, Regulates Plant Height by Functionally Overlapping with <i>DEP1</i> in Rice
Plant heterotrimeric G proteins have been shown to regulate the size of various organs. There are three types of Gγ subunits in plants: type A, consisting of a canonical Gγ domain; type B, possessing a plant-specific domain at the N-terminus of the Gγ domain; and type C, possessing a plant-specific...
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MDPI AG
2022-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/3/422 |
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| author | Genki Chaya Shuhei Segami Moeka Fujita Yoichi Morinaka Yukimoto Iwasaki Kotaro Miura |
| author_facet | Genki Chaya Shuhei Segami Moeka Fujita Yoichi Morinaka Yukimoto Iwasaki Kotaro Miura |
| author_sort | Genki Chaya |
| collection | DOAJ |
| description | Plant heterotrimeric G proteins have been shown to regulate the size of various organs. There are three types of Gγ subunits in plants: type A, consisting of a canonical Gγ domain; type B, possessing a plant-specific domain at the N-terminus of the Gγ domain; and type C, possessing a plant-specific domain at the C-terminal of the Gγ domain. There is one type A, one type B, and three type C of the five γ-subunits in the rice genome. In type C Gγ subunits, <i>GS3</i>, which controls grain size; <i>DEP1</i>, which controls plant height and panicle branching; and their homolog <i>OsGGC2</i>, which affects grain size, have been reported; however, the function of each gene, their interactions, and molecular mechanisms for the control of plant height have not yet been clarified. In this study, we generated loss-of-function mutants of <i>DEP1</i> and <i>OsGGC2</i>, which have high homology and similar expression, and investigated their phenotypes. Since both <i>dep1</i> and <i>osggc2</i> mutants were dwarfed and the double mutants showed a synergistic phenotype, we concluded that both <i>DEP1</i> and <i>OsGGC2</i> are positive regulators of plant height and that their functions are redundant. |
| first_indexed | 2024-03-09T23:18:15Z |
| format | Article |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T23:18:15Z |
| publishDate | 2022-02-01 |
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| series | Plants |
| spelling | doaj.art-1b306998fed74aa695faa6c6a2b4aa612023-11-23T17:31:44ZengMDPI AGPlants2223-77472022-02-0111342210.3390/plants11030422<i>OsGGC2</i>, Gγ Subunit of Heterotrimeric G Protein, Regulates Plant Height by Functionally Overlapping with <i>DEP1</i> in RiceGenki Chaya0Shuhei Segami1Moeka Fujita2Yoichi Morinaka3Yukimoto Iwasaki4Kotaro Miura5Faculty of Bioscience and Biotechnology, Fukui Prefectural University, 4-1-1 Kenjojima, Matsuoka, Yoshida-gun, Eiheiji-cho 910-1195, Fukui, JapanResearch Institute of Environment, Agriculture and Fisheries, 442 Shakudo, Habikino 583-0862, Osaka, JapanFaculty of Bioscience and Biotechnology, Fukui Prefectural University, 4-1-1 Kenjojima, Matsuoka, Yoshida-gun, Eiheiji-cho 910-1195, Fukui, JapanFaculty of Bioscience and Biotechnology, Fukui Prefectural University, 4-1-1 Kenjojima, Matsuoka, Yoshida-gun, Eiheiji-cho 910-1195, Fukui, JapanFaculty of Bioscience and Biotechnology, Fukui Prefectural University, 4-1-1 Kenjojima, Matsuoka, Yoshida-gun, Eiheiji-cho 910-1195, Fukui, JapanFaculty of Bioscience and Biotechnology, Fukui Prefectural University, 4-1-1 Kenjojima, Matsuoka, Yoshida-gun, Eiheiji-cho 910-1195, Fukui, JapanPlant heterotrimeric G proteins have been shown to regulate the size of various organs. There are three types of Gγ subunits in plants: type A, consisting of a canonical Gγ domain; type B, possessing a plant-specific domain at the N-terminus of the Gγ domain; and type C, possessing a plant-specific domain at the C-terminal of the Gγ domain. There is one type A, one type B, and three type C of the five γ-subunits in the rice genome. In type C Gγ subunits, <i>GS3</i>, which controls grain size; <i>DEP1</i>, which controls plant height and panicle branching; and their homolog <i>OsGGC2</i>, which affects grain size, have been reported; however, the function of each gene, their interactions, and molecular mechanisms for the control of plant height have not yet been clarified. In this study, we generated loss-of-function mutants of <i>DEP1</i> and <i>OsGGC2</i>, which have high homology and similar expression, and investigated their phenotypes. Since both <i>dep1</i> and <i>osggc2</i> mutants were dwarfed and the double mutants showed a synergistic phenotype, we concluded that both <i>DEP1</i> and <i>OsGGC2</i> are positive regulators of plant height and that their functions are redundant.https://www.mdpi.com/2223-7747/11/3/422riceheterotrimeric G protein<i>DEP1</i><i>OsGGC2</i> |
| spellingShingle | Genki Chaya Shuhei Segami Moeka Fujita Yoichi Morinaka Yukimoto Iwasaki Kotaro Miura <i>OsGGC2</i>, Gγ Subunit of Heterotrimeric G Protein, Regulates Plant Height by Functionally Overlapping with <i>DEP1</i> in Rice Plants rice heterotrimeric G protein <i>DEP1</i> <i>OsGGC2</i> |
| title | <i>OsGGC2</i>, Gγ Subunit of Heterotrimeric G Protein, Regulates Plant Height by Functionally Overlapping with <i>DEP1</i> in Rice |
| title_full | <i>OsGGC2</i>, Gγ Subunit of Heterotrimeric G Protein, Regulates Plant Height by Functionally Overlapping with <i>DEP1</i> in Rice |
| title_fullStr | <i>OsGGC2</i>, Gγ Subunit of Heterotrimeric G Protein, Regulates Plant Height by Functionally Overlapping with <i>DEP1</i> in Rice |
| title_full_unstemmed | <i>OsGGC2</i>, Gγ Subunit of Heterotrimeric G Protein, Regulates Plant Height by Functionally Overlapping with <i>DEP1</i> in Rice |
| title_short | <i>OsGGC2</i>, Gγ Subunit of Heterotrimeric G Protein, Regulates Plant Height by Functionally Overlapping with <i>DEP1</i> in Rice |
| title_sort | i osggc2 i gγ subunit of heterotrimeric g protein regulates plant height by functionally overlapping with i dep1 i in rice |
| topic | rice heterotrimeric G protein <i>DEP1</i> <i>OsGGC2</i> |
| url | https://www.mdpi.com/2223-7747/11/3/422 |
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