The influences of purple sweet potato anthocyanin on the growth characteristics of human retinal pigment epithelial cells
Background: Anthocyanins have been proven to be beneficial to the eyes. However, information is scarce about the effects of purple sweet potato (Ipomoea batatas, L.) anthocyanin (PSPA), a class of anthocyanins derived from purple sweet potato roots, on visual health. Objective: The aim of this study...
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| Format: | Article |
| Language: | English |
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Swedish Nutrition Foundation
2015-06-01
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| Series: | Food & Nutrition Research |
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| Online Access: | http://www.foodandnutritionresearch.net/index.php/fnr/article/view/27830/40619 |
| _version_ | 1818264580073193472 |
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| author | Min Sun Xiaoling Lu Lei Hao Tao Wu Huanjiao Zhao Chao Wang |
| author_facet | Min Sun Xiaoling Lu Lei Hao Tao Wu Huanjiao Zhao Chao Wang |
| author_sort | Min Sun |
| collection | DOAJ |
| description | Background: Anthocyanins have been proven to be beneficial to the eyes. However, information is scarce about the effects of purple sweet potato (Ipomoea batatas, L.) anthocyanin (PSPA), a class of anthocyanins derived from purple sweet potato roots, on visual health. Objective: The aim of this study was to investigate whether PSPA could have influences on the growth characteristics (cellular morphology, survival, and proliferation) of human retinal pigment epithelial (RPE) cells, which perform essential functions for the visual process. Methods: The RPE cell line D407 was used in the present study. The cytotoxicity of PSPA was assessed by MTT assay. Then, cellular morphology, viability, cell cycle, Ki67expression, and PI3K/MAPK activation of RPE cells treated with PSPA were determined. Results: PSPA exhibited dose-dependent promotion of RPE cell proliferation at concentrations ranging from 10 to 1,000 µg/ml. RPE cells treated with PSPA demonstrated a predominantly polygonal morphology in a mosaic arrangement, and colony-like cells displayed numerous short apical microvilli and typical ultrastructure. PSPA treatment also resulted in a better platform growing status, statistically higher viability, an increase in the S-phase, and more Ki67+ cells. However, neither pAkt nor pERK were detected in either group. Conclusions: We found that PSPA maintained high cell viability, boosted DNA synthesis, and preserved a high percentage of continuously cycling cells to promote cell survival and division without changing cell morphology. This paper lays the foundation for further research about the damage-protective activities of PSPA on RPE cells or human vision. |
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| institution | Directory Open Access Journal |
| issn | 1654-661X |
| language | English |
| last_indexed | 2024-12-12T19:37:10Z |
| publishDate | 2015-06-01 |
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| series | Food & Nutrition Research |
| spelling | doaj.art-1b317e65af4d425abc1f341789077cc12022-12-22T00:14:17ZengSwedish Nutrition FoundationFood & Nutrition Research1654-661X2015-06-015901910.3402/fnr.v59.2783027830The influences of purple sweet potato anthocyanin on the growth characteristics of human retinal pigment epithelial cellsMin Sun0Xiaoling Lu1Lei Hao2Tao Wu3Huanjiao Zhao4Chao Wang5Key Laboratory of Food Nutrition and Safety of the Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin, ChinaKey Laboratory of Food Nutrition and Safety of the Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin, ChinaKey Laboratory of Food Nutrition and Safety of the Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin, ChinaKey Laboratory of Food Nutrition and Safety of the Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin, ChinaKey Laboratory of Food Nutrition and Safety of the Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin, ChinaKey Laboratory of Food Nutrition and Safety of the Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin, ChinaBackground: Anthocyanins have been proven to be beneficial to the eyes. However, information is scarce about the effects of purple sweet potato (Ipomoea batatas, L.) anthocyanin (PSPA), a class of anthocyanins derived from purple sweet potato roots, on visual health. Objective: The aim of this study was to investigate whether PSPA could have influences on the growth characteristics (cellular morphology, survival, and proliferation) of human retinal pigment epithelial (RPE) cells, which perform essential functions for the visual process. Methods: The RPE cell line D407 was used in the present study. The cytotoxicity of PSPA was assessed by MTT assay. Then, cellular morphology, viability, cell cycle, Ki67expression, and PI3K/MAPK activation of RPE cells treated with PSPA were determined. Results: PSPA exhibited dose-dependent promotion of RPE cell proliferation at concentrations ranging from 10 to 1,000 µg/ml. RPE cells treated with PSPA demonstrated a predominantly polygonal morphology in a mosaic arrangement, and colony-like cells displayed numerous short apical microvilli and typical ultrastructure. PSPA treatment also resulted in a better platform growing status, statistically higher viability, an increase in the S-phase, and more Ki67+ cells. However, neither pAkt nor pERK were detected in either group. Conclusions: We found that PSPA maintained high cell viability, boosted DNA synthesis, and preserved a high percentage of continuously cycling cells to promote cell survival and division without changing cell morphology. This paper lays the foundation for further research about the damage-protective activities of PSPA on RPE cells or human vision.http://www.foodandnutritionresearch.net/index.php/fnr/article/view/27830/40619PSPARPE cellsmorphologysurvivalproliferation |
| spellingShingle | Min Sun Xiaoling Lu Lei Hao Tao Wu Huanjiao Zhao Chao Wang The influences of purple sweet potato anthocyanin on the growth characteristics of human retinal pigment epithelial cells Food & Nutrition Research PSPA RPE cells morphology survival proliferation |
| title | The influences of purple sweet potato anthocyanin on the growth characteristics of human retinal pigment epithelial cells |
| title_full | The influences of purple sweet potato anthocyanin on the growth characteristics of human retinal pigment epithelial cells |
| title_fullStr | The influences of purple sweet potato anthocyanin on the growth characteristics of human retinal pigment epithelial cells |
| title_full_unstemmed | The influences of purple sweet potato anthocyanin on the growth characteristics of human retinal pigment epithelial cells |
| title_short | The influences of purple sweet potato anthocyanin on the growth characteristics of human retinal pigment epithelial cells |
| title_sort | influences of purple sweet potato anthocyanin on the growth characteristics of human retinal pigment epithelial cells |
| topic | PSPA RPE cells morphology survival proliferation |
| url | http://www.foodandnutritionresearch.net/index.php/fnr/article/view/27830/40619 |
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