A quality improvement study on the relationship between intranasal povidone-iodine and anesthesia and the nasal microbiota of surgery patients
<h4>Introduction</h4> The composition of the nasal microbiota in surgical patients in the context of general anesthesia and nasal povidone-iodine decolonization is unknown. The purpose of this quality improvement study was to determine: (i) if general anesthesia is associated with change...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733847/?tool=EBI |
| _version_ | 1811178105264406528 |
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| author | Eric N. Hammond Ashley E. Kates Nathan Putman-Buehler Lauren Watson Jared J. Godfrey Nicole Brys Courtney Deblois Andrew J. Steinberger Madison S. Cox Joseph H. Skarlupka Ambar Haleem Michael L. Bentz Garret Suen Nasia Safdar |
| author_facet | Eric N. Hammond Ashley E. Kates Nathan Putman-Buehler Lauren Watson Jared J. Godfrey Nicole Brys Courtney Deblois Andrew J. Steinberger Madison S. Cox Joseph H. Skarlupka Ambar Haleem Michael L. Bentz Garret Suen Nasia Safdar |
| author_sort | Eric N. Hammond |
| collection | DOAJ |
| description | <h4>Introduction</h4> The composition of the nasal microbiota in surgical patients in the context of general anesthesia and nasal povidone-iodine decolonization is unknown. The purpose of this quality improvement study was to determine: (i) if general anesthesia is associated with changes in the nasal microbiota of surgery patients and (ii) if preoperative intranasal povidone-iodine decolonization is associated with changes in the nasal microbiota of surgery patients. <h4>Materials and methods</h4> One hundred and fifty-one ambulatory patients presenting for surgery were enrolled in a quality improvement study by convenience sampling. Pre- and post-surgery nasal samples were collected from patients in the no intranasal decolonization group (control group, n = 54). Pre-decolonization nasal samples were collected from the preoperative intranasal povidone-iodine decolonization group (povidone-iodine group, n = 97). Intranasal povidone-iodine was administered immediately prior to surgery and continued for 20 minutes before patients proceeded for surgery. Post-nasal samples were then collected. General anesthesia was administered to both groups. DNA from the samples was extracted for 16S rRNA sequencing on an Illumina MiSeq. <h4>Results</h4> In the control group, there was no evidence of change in bacterial diversity between pre- and post-surgery samples. In the povidone-iodine group, nasal bacterial diversity was greater in post-surgery, relative to pre-surgery (Shannon’s Diversity Index (P = 0.038), Chao’s richness estimate (P = 0.02) and Inverse Simpson index (P = 0.027). Among all the genera, only the relative abundance of the genus Staphylococcus trended towards a decrease in patients after application (FDR adjusted P = 0.06). Abundant genera common to both povidone-iodine and control groups included Staphylococcus, Bradyrhizobium, Corynebacterium, Dolosigranulum, Lactobacillus, and Moraxella. <h4>Conclusions</h4> We found general anesthesia was not associated with changes in the nasal microbiota. Povidone-iodine treatment was associated with nasal microbial diversity and decreased abundance of Staphylococcus. Future studies should examine the nasal microbiota structure and function longitudinally in surgical patients receiving intranasal povidone-iodine. |
| first_indexed | 2024-04-11T06:12:43Z |
| format | Article |
| id | doaj.art-1b35bdc4b6c74b8398d45a9fc393811b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T06:12:43Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-1b35bdc4b6c74b8398d45a9fc393811b2022-12-22T04:41:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011712A quality improvement study on the relationship between intranasal povidone-iodine and anesthesia and the nasal microbiota of surgery patientsEric N. HammondAshley E. KatesNathan Putman-BuehlerLauren WatsonJared J. GodfreyNicole BrysCourtney DebloisAndrew J. SteinbergerMadison S. CoxJoseph H. SkarlupkaAmbar HaleemMichael L. BentzGarret SuenNasia Safdar<h4>Introduction</h4> The composition of the nasal microbiota in surgical patients in the context of general anesthesia and nasal povidone-iodine decolonization is unknown. The purpose of this quality improvement study was to determine: (i) if general anesthesia is associated with changes in the nasal microbiota of surgery patients and (ii) if preoperative intranasal povidone-iodine decolonization is associated with changes in the nasal microbiota of surgery patients. <h4>Materials and methods</h4> One hundred and fifty-one ambulatory patients presenting for surgery were enrolled in a quality improvement study by convenience sampling. Pre- and post-surgery nasal samples were collected from patients in the no intranasal decolonization group (control group, n = 54). Pre-decolonization nasal samples were collected from the preoperative intranasal povidone-iodine decolonization group (povidone-iodine group, n = 97). Intranasal povidone-iodine was administered immediately prior to surgery and continued for 20 minutes before patients proceeded for surgery. Post-nasal samples were then collected. General anesthesia was administered to both groups. DNA from the samples was extracted for 16S rRNA sequencing on an Illumina MiSeq. <h4>Results</h4> In the control group, there was no evidence of change in bacterial diversity between pre- and post-surgery samples. In the povidone-iodine group, nasal bacterial diversity was greater in post-surgery, relative to pre-surgery (Shannon’s Diversity Index (P = 0.038), Chao’s richness estimate (P = 0.02) and Inverse Simpson index (P = 0.027). Among all the genera, only the relative abundance of the genus Staphylococcus trended towards a decrease in patients after application (FDR adjusted P = 0.06). Abundant genera common to both povidone-iodine and control groups included Staphylococcus, Bradyrhizobium, Corynebacterium, Dolosigranulum, Lactobacillus, and Moraxella. <h4>Conclusions</h4> We found general anesthesia was not associated with changes in the nasal microbiota. Povidone-iodine treatment was associated with nasal microbial diversity and decreased abundance of Staphylococcus. Future studies should examine the nasal microbiota structure and function longitudinally in surgical patients receiving intranasal povidone-iodine.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733847/?tool=EBI |
| spellingShingle | Eric N. Hammond Ashley E. Kates Nathan Putman-Buehler Lauren Watson Jared J. Godfrey Nicole Brys Courtney Deblois Andrew J. Steinberger Madison S. Cox Joseph H. Skarlupka Ambar Haleem Michael L. Bentz Garret Suen Nasia Safdar A quality improvement study on the relationship between intranasal povidone-iodine and anesthesia and the nasal microbiota of surgery patients PLoS ONE |
| title | A quality improvement study on the relationship between intranasal povidone-iodine and anesthesia and the nasal microbiota of surgery patients |
| title_full | A quality improvement study on the relationship between intranasal povidone-iodine and anesthesia and the nasal microbiota of surgery patients |
| title_fullStr | A quality improvement study on the relationship between intranasal povidone-iodine and anesthesia and the nasal microbiota of surgery patients |
| title_full_unstemmed | A quality improvement study on the relationship between intranasal povidone-iodine and anesthesia and the nasal microbiota of surgery patients |
| title_short | A quality improvement study on the relationship between intranasal povidone-iodine and anesthesia and the nasal microbiota of surgery patients |
| title_sort | quality improvement study on the relationship between intranasal povidone iodine and anesthesia and the nasal microbiota of surgery patients |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733847/?tool=EBI |
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