NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence
Abstract The NAM, ATAF1/2, and CUC2 (NAC) domain transcription factor VND‐INTERACTING2 (VNI2) negatively regulates xylem vessel formation by interacting with another NAC domain transcription factor, VASCULAR‐RELATED NAC‐DOMAIN7 (VND7), a master regulator of xylem vessel formation. Here, we screened...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-09-01
|
| Series: | Plant Direct |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pld3.529 |
| _version_ | 1797629637462851584 |
|---|---|
| author | Isura Sumeda Priyadarshana Nagahage Kohei Matsuda Kyoko Miyashita Sumire Fujiwara Chanaka Mannapperuma Takuya Yamada Shingo Sakamoto Toshiki Ishikawa Minoru Nagano Misato Ohtani Ko Kato Hirofumi Uchimiya Nobutaka Mitsuda Maki Kawai‐Yamada Taku Demura Masatoshi Yamaguchi |
| author_facet | Isura Sumeda Priyadarshana Nagahage Kohei Matsuda Kyoko Miyashita Sumire Fujiwara Chanaka Mannapperuma Takuya Yamada Shingo Sakamoto Toshiki Ishikawa Minoru Nagano Misato Ohtani Ko Kato Hirofumi Uchimiya Nobutaka Mitsuda Maki Kawai‐Yamada Taku Demura Masatoshi Yamaguchi |
| author_sort | Isura Sumeda Priyadarshana Nagahage |
| collection | DOAJ |
| description | Abstract The NAM, ATAF1/2, and CUC2 (NAC) domain transcription factor VND‐INTERACTING2 (VNI2) negatively regulates xylem vessel formation by interacting with another NAC domain transcription factor, VASCULAR‐RELATED NAC‐DOMAIN7 (VND7), a master regulator of xylem vessel formation. Here, we screened interacting proteins with VNI2 using yeast two‐hybrid assay and isolated two NAC domain transcription factors, Arabidopsis thaliana ACTIVATION FACTOR 2 (ATAF2) and NAC DOMAIN CONTAINING PROTEIN 102 (ANAC102). A transient gene expression assay showed that ATAF2 upregulates the expression of genes involved in leaf senescence, and VNI2 effectively inhibits the transcriptional activation activity of ATAF2. vni2 mutants accelerate leaf senescence, whereas ataf2 mutants delay leaf senescence. In addition, the accelerated leaf senescence phenotype of the vni2 mutant is recovered by simultaneous mutation of ATAF2. Our findings strongly suggest that VNI2 interacts with and inhibits ATAF2, resulting in negatively regulating leaf senescence. |
| first_indexed | 2024-03-11T10:55:56Z |
| format | Article |
| id | doaj.art-1b39def2544d4ef3bd0f7f568a5b543f |
| institution | Directory Open Access Journal |
| issn | 2475-4455 |
| language | English |
| last_indexed | 2024-03-11T10:55:56Z |
| publishDate | 2023-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj.art-1b39def2544d4ef3bd0f7f568a5b543f2023-11-13T07:55:57ZengWileyPlant Direct2475-44552023-09-0179n/an/a10.1002/pld3.529NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescenceIsura Sumeda Priyadarshana Nagahage0Kohei Matsuda1Kyoko Miyashita2Sumire Fujiwara3Chanaka Mannapperuma4Takuya Yamada5Shingo Sakamoto6Toshiki Ishikawa7Minoru Nagano8Misato Ohtani9Ko Kato10Hirofumi Uchimiya11Nobutaka Mitsuda12Maki Kawai‐Yamada13Taku Demura14Masatoshi Yamaguchi15Graduate School of Science and Engineering Saitama University Saitama JapanGraduate School of Science and Technology Nara Institute of Science and Technology Ikoma JapanBioproduction Research Institute National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba JapanBioproduction Research Institute National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba JapanUmeå Plant Science Centre, Department of Plant Physiology Umeå University Umeå SwedenGraduate School of Science and Engineering Saitama University Saitama JapanBioproduction Research Institute National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba JapanGraduate School of Science and Engineering Saitama University Saitama JapanGraduate School of Science and Engineering Saitama University Saitama JapanGraduate School of Science and Technology Nara Institute of Science and Technology Ikoma JapanGraduate School of Science and Technology Nara Institute of Science and Technology Ikoma JapanInstitute for Environmental Science and Technology Saitama University Saitama JapanBioproduction Research Institute National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba JapanGraduate School of Science and Engineering Saitama University Saitama JapanGraduate School of Science and Technology Nara Institute of Science and Technology Ikoma JapanGraduate School of Science and Engineering Saitama University Saitama JapanAbstract The NAM, ATAF1/2, and CUC2 (NAC) domain transcription factor VND‐INTERACTING2 (VNI2) negatively regulates xylem vessel formation by interacting with another NAC domain transcription factor, VASCULAR‐RELATED NAC‐DOMAIN7 (VND7), a master regulator of xylem vessel formation. Here, we screened interacting proteins with VNI2 using yeast two‐hybrid assay and isolated two NAC domain transcription factors, Arabidopsis thaliana ACTIVATION FACTOR 2 (ATAF2) and NAC DOMAIN CONTAINING PROTEIN 102 (ANAC102). A transient gene expression assay showed that ATAF2 upregulates the expression of genes involved in leaf senescence, and VNI2 effectively inhibits the transcriptional activation activity of ATAF2. vni2 mutants accelerate leaf senescence, whereas ataf2 mutants delay leaf senescence. In addition, the accelerated leaf senescence phenotype of the vni2 mutant is recovered by simultaneous mutation of ATAF2. Our findings strongly suggest that VNI2 interacts with and inhibits ATAF2, resulting in negatively regulating leaf senescence.https://doi.org/10.1002/pld3.529Arabidopsis thalianaleaf senescenceNAC domain proteinprotein–protein interactiontranscription factor |
| spellingShingle | Isura Sumeda Priyadarshana Nagahage Kohei Matsuda Kyoko Miyashita Sumire Fujiwara Chanaka Mannapperuma Takuya Yamada Shingo Sakamoto Toshiki Ishikawa Minoru Nagano Misato Ohtani Ko Kato Hirofumi Uchimiya Nobutaka Mitsuda Maki Kawai‐Yamada Taku Demura Masatoshi Yamaguchi NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence Plant Direct Arabidopsis thaliana leaf senescence NAC domain protein protein–protein interaction transcription factor |
| title | NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence |
| title_full | NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence |
| title_fullStr | NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence |
| title_full_unstemmed | NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence |
| title_short | NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence |
| title_sort | nac domain transcription factors vni2 and ataf2 form protein complexes and regulate leaf senescence |
| topic | Arabidopsis thaliana leaf senescence NAC domain protein protein–protein interaction transcription factor |
| url | https://doi.org/10.1002/pld3.529 |
| work_keys_str_mv | AT isurasumedapriyadarshananagahage nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT koheimatsuda nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT kyokomiyashita nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT sumirefujiwara nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT chanakamannapperuma nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT takuyayamada nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT shingosakamoto nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT toshikiishikawa nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT minorunagano nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT misatoohtani nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT kokato nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT hirofumiuchimiya nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT nobutakamitsuda nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT makikawaiyamada nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT takudemura nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence AT masatoshiyamaguchi nacdomaintranscriptionfactorsvni2andataf2formproteincomplexesandregulateleafsenescence |