Intratumor heterogeneity in epithelial cancer: Assessment in cell line models and circulating tumor cells
Background: CTCs are present only in small numbers in patients' blood. This study aimed to establish a protocol for enumeration and phenotypic characterization of circulating tumor cells (CTCs) by ImageStreamX MK II (AMNIS) imaging flow cytometry and to characterize the expression of epithelial...
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2019-04-01
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| Series: | Makara Journal of Health Research |
| Subjects: | |
| Online Access: | https://scholarhub.ui.ac.id/mjhr/vol23/iss1/9/ |
| _version_ | 1797893821988601856 |
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| author | Wimba W. Dinutanayo Ingeborg T. Keilholz Diana Braunholz |
| author_facet | Wimba W. Dinutanayo Ingeborg T. Keilholz Diana Braunholz |
| author_sort | Wimba W. Dinutanayo |
| collection | DOAJ |
| description | Background: CTCs are present only in small numbers in patients' blood. This study aimed to establish a protocol for enumeration and phenotypic characterization of circulating tumor cells (CTCs) by ImageStreamX MK II (AMNIS) imaging flow cytometry and to characterize the expression of epithelial, mesenchymal, and stem cell markers in CTCs. Methods: The study used the FaDu cell line at different passages, cisplatin-resistant (FaDu CDDP-R), and irradiation-resistant (FaDu IR-R) subclones, as well as blood samples from head and neck squamous cell carcinoma (HNSCC) and colorectal cancer (CRC) patients for CTC detection (n = 5). Cells were fixed using 4% paraformaldehyde and permeabilized by incubation in 0.3% Triton X-100. The cell suspensions were stained with 1:100 EpCAM AF-488, 1:50 CD45 AF-647, 1:50 Vimentin AF-555, and 1:50 ALDH1A AF-594 antibodies. Results: There were significant differences in EpCAM expression levels between FaDu at late passage and FaDu CDDP-R subclones, as well as between FaDu at late passage compared with FaDu IR-R. Furthermore, CTCs were successfully detected in five patients' samples with various CTC subpopulations. Conclusions: Intratumor heterogeneity in CTC phenotypes existed in CRC and HNSCC. Furthermore, three main subpopulations of CTCs were detected. Our findings strongly support future phenotypic studies of CTCs. |
| first_indexed | 2024-04-10T06:59:11Z |
| format | Article |
| id | doaj.art-1b3b2fcf84a34a33b585517bdc82f2e2 |
| institution | Directory Open Access Journal |
| issn | 2356-3664 2356-3656 |
| language | English |
| last_indexed | 2024-04-10T06:59:11Z |
| publishDate | 2019-04-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Journal of Health Research |
| spelling | doaj.art-1b3b2fcf84a34a33b585517bdc82f2e22023-02-28T02:41:28ZengUniversitas IndonesiaMakara Journal of Health Research2356-36642356-36562019-04-01231536310.7454/msk.v23i1.10149Intratumor heterogeneity in epithelial cancer: Assessment in cell line models and circulating tumor cellsWimba W. Dinutanayo0Ingeborg T. Keilholz1Diana Braunholz2Faculty of Health, Medicine, and Life Science, Maastricht University, the NetherlandsTranslational Radiation Oncology Research Laboratory, Department of Radiation Oncology and Radiotherapy, Charité Universitätsmedizin Berlin, GermanyTranslational Radiation Oncology Research Laboratory, Department of Radiation Oncology and Radiotherapy, Charité Universitätsmedizin Berlin, GermanyBackground: CTCs are present only in small numbers in patients' blood. This study aimed to establish a protocol for enumeration and phenotypic characterization of circulating tumor cells (CTCs) by ImageStreamX MK II (AMNIS) imaging flow cytometry and to characterize the expression of epithelial, mesenchymal, and stem cell markers in CTCs. Methods: The study used the FaDu cell line at different passages, cisplatin-resistant (FaDu CDDP-R), and irradiation-resistant (FaDu IR-R) subclones, as well as blood samples from head and neck squamous cell carcinoma (HNSCC) and colorectal cancer (CRC) patients for CTC detection (n = 5). Cells were fixed using 4% paraformaldehyde and permeabilized by incubation in 0.3% Triton X-100. The cell suspensions were stained with 1:100 EpCAM AF-488, 1:50 CD45 AF-647, 1:50 Vimentin AF-555, and 1:50 ALDH1A AF-594 antibodies. Results: There were significant differences in EpCAM expression levels between FaDu at late passage and FaDu CDDP-R subclones, as well as between FaDu at late passage compared with FaDu IR-R. Furthermore, CTCs were successfully detected in five patients' samples with various CTC subpopulations. Conclusions: Intratumor heterogeneity in CTC phenotypes existed in CRC and HNSCC. Furthermore, three main subpopulations of CTCs were detected. Our findings strongly support future phenotypic studies of CTCs.https://scholarhub.ui.ac.id/mjhr/vol23/iss1/9/cancer stem cellcirculating tumor cellepithelial-mesenchymal transitionimaging flow cytometryliquid biopsyneoplastic cells |
| spellingShingle | Wimba W. Dinutanayo Ingeborg T. Keilholz Diana Braunholz Intratumor heterogeneity in epithelial cancer: Assessment in cell line models and circulating tumor cells Makara Journal of Health Research cancer stem cell circulating tumor cell epithelial-mesenchymal transition imaging flow cytometry liquid biopsy neoplastic cells |
| title | Intratumor heterogeneity in epithelial cancer: Assessment in cell line models and circulating tumor cells |
| title_full | Intratumor heterogeneity in epithelial cancer: Assessment in cell line models and circulating tumor cells |
| title_fullStr | Intratumor heterogeneity in epithelial cancer: Assessment in cell line models and circulating tumor cells |
| title_full_unstemmed | Intratumor heterogeneity in epithelial cancer: Assessment in cell line models and circulating tumor cells |
| title_short | Intratumor heterogeneity in epithelial cancer: Assessment in cell line models and circulating tumor cells |
| title_sort | intratumor heterogeneity in epithelial cancer assessment in cell line models and circulating tumor cells |
| topic | cancer stem cell circulating tumor cell epithelial-mesenchymal transition imaging flow cytometry liquid biopsy neoplastic cells |
| url | https://scholarhub.ui.ac.id/mjhr/vol23/iss1/9/ |
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