Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions
In this study we studied the kinetic interaction of indole-3- acetic acid with human serum butyrylcholinesterase. Butyrylcholinesterase was purified from human serum and the benzoylcholine kinetics was analyzed. The enzyme had a Km value of 14.22 ± 2.97 μM. The Hill plot was linear with a value of n...
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2006-12-01
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| Series: | Türk Biyokimya Dergisi |
| Subjects: | |
| Online Access: | http://www.turkjbiochem.com/2006/182.pdf |
| _version_ | 1797924310365503488 |
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| author | Ebru Bodur, A. Neşe Çokuğraş |
| author_facet | Ebru Bodur, A. Neşe Çokuğraş |
| author_sort | Ebru Bodur, |
| collection | DOAJ |
| description | In this study we studied the kinetic interaction of indole-3- acetic acid with human serum butyrylcholinesterase. Butyrylcholinesterase was purified from human serum and the benzoylcholine kinetics was analyzed. The enzyme had a Km value of 14.22 ± 2.97 μM. The Hill plot was linear with a value of nH = 0.95, displaying that the enzyme follows Michaelis-Menten kinetics with regard to benzoylcholine. Through inhibition studies, indole-3-acetic acid was found to be a noncompetitive inhibitor for human serum butyrylcholinesterase with benzoylcholineas substrate. The Ki value was calculated as 1.86 ± 0.27 mM. In our previous study on human serum butyrylcholinesterase using butyrylthiocholine as substrate, indole-3-acetic acid was found to be a linear-mixed type inhibitor.This difference in kinetic behavior with regards to two different substrates could arise from the tighter binding of the more bulky and hydrophobic benzoyl group to the hydrophobic pocket of the active site gorge rather than the butyryl group and that the binding of this butyryl group could be sterically hindered by the indole-3-acetic acid. |
| first_indexed | 2024-04-10T14:59:32Z |
| format | Article |
| id | doaj.art-1b3cbd9d771b457d90d83d6c37a43fa7 |
| institution | Directory Open Access Journal |
| issn | 0250-4685 1303-829X |
| language | English |
| last_indexed | 2024-04-10T14:59:32Z |
| publishDate | 2006-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Türk Biyokimya Dergisi |
| spelling | doaj.art-1b3cbd9d771b457d90d83d6c37a43fa72023-02-15T16:07:06ZengDe GruyterTürk Biyokimya Dergisi0250-46851303-829X2006-12-01314182186Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase InteractionsEbru Bodur,A. Neşe ÇokuğraşIn this study we studied the kinetic interaction of indole-3- acetic acid with human serum butyrylcholinesterase. Butyrylcholinesterase was purified from human serum and the benzoylcholine kinetics was analyzed. The enzyme had a Km value of 14.22 ± 2.97 μM. The Hill plot was linear with a value of nH = 0.95, displaying that the enzyme follows Michaelis-Menten kinetics with regard to benzoylcholine. Through inhibition studies, indole-3-acetic acid was found to be a noncompetitive inhibitor for human serum butyrylcholinesterase with benzoylcholineas substrate. The Ki value was calculated as 1.86 ± 0.27 mM. In our previous study on human serum butyrylcholinesterase using butyrylthiocholine as substrate, indole-3-acetic acid was found to be a linear-mixed type inhibitor.This difference in kinetic behavior with regards to two different substrates could arise from the tighter binding of the more bulky and hydrophobic benzoyl group to the hydrophobic pocket of the active site gorge rather than the butyryl group and that the binding of this butyryl group could be sterically hindered by the indole-3-acetic acid.http://www.turkjbiochem.com/2006/182.pdfBenzoylcholinebutyrylcholinesteraseindole-acetic acidinhibition |
| spellingShingle | Ebru Bodur, A. Neşe Çokuğraş Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions Türk Biyokimya Dergisi Benzoylcholine butyrylcholinesterase indole-acetic acid inhibition |
| title | Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions |
| title_full | Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions |
| title_fullStr | Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions |
| title_full_unstemmed | Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions |
| title_short | Benzoylcholine, Indole-3-acetic And Human Serum Butyrylcholinesterase Interactions |
| title_sort | benzoylcholine indole 3 acetic and human serum butyrylcholinesterase interactions |
| topic | Benzoylcholine butyrylcholinesterase indole-acetic acid inhibition |
| url | http://www.turkjbiochem.com/2006/182.pdf |
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