Establishment and Optimization of Micropropagation System for Southern Highbush Blueberry
The optimization of micropropagation for blueberries is crucial due to the growing blueberry industry and demand for plantlets. This study focused on four stages: explant sterilization, in vitro establishment, in vitro proliferation, and ex vitro rooting, aiming to establish an efficient in vitro pr...
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| Format: | Article |
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MDPI AG
2023-08-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/8/893 |
| _version_ | 1797584537188827136 |
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| author | Yuting Wang Xiaoyun Zhang Zhehao Jiang Xiaolong Yang Xiaojuan Liu Xi Ou Wei Su Riyuan Chen |
| author_facet | Yuting Wang Xiaoyun Zhang Zhehao Jiang Xiaolong Yang Xiaojuan Liu Xi Ou Wei Su Riyuan Chen |
| author_sort | Yuting Wang |
| collection | DOAJ |
| description | The optimization of micropropagation for blueberries is crucial due to the growing blueberry industry and demand for plantlets. This study focused on four stages: explant sterilization, in vitro establishment, in vitro proliferation, and ex vitro rooting, aiming to establish an efficient in vitro propagation system for southern highbush blueberry cultivar ‘ZY09’. The most effective explant sterilization method was a 60 s treatment with 75% ethanol and a 5 min treatment with 4% NaClO. During the establishment of the in vitro culture, the modified woody plant medium was found to be suitable. The replacement of NH<sub>4</sub>NO<sub>3</sub> in woody plant medium with (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> facilitated the proliferation of blueberry microshoots. The optimal combination of plant growth regulators for the in vitro proliferation of blueberry microshoots was indole-3-butyric acid (0.1 mg·L<sup>−1</sup>), thidiazuron (0.0005 mg·L<sup>−1</sup>), and zeatin (1 mg·L<sup>−1</sup>). Perlite was the most suitable substrate for ex vitro rooting. The best ex vitro rooting performance was observed without immersion in growth regulators. Indole-3-butyric acid enhances root formation and suppresses root elongation in blueberries. The findings of this study can be applied to large-scale in vitro propagation of southern highbush blueberry and provide a reference for the genetic transformation of blueberries. |
| first_indexed | 2024-03-10T23:53:05Z |
| format | Article |
| id | doaj.art-1b3d57f1fd674f749dbb06f6c103a41b |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T23:53:05Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-1b3d57f1fd674f749dbb06f6c103a41b2023-11-19T01:21:19ZengMDPI AGHorticulturae2311-75242023-08-019889310.3390/horticulturae9080893Establishment and Optimization of Micropropagation System for Southern Highbush BlueberryYuting Wang0Xiaoyun Zhang1Zhehao Jiang2Xiaolong Yang3Xiaojuan Liu4Xi Ou5Wei Su6Riyuan Chen7College of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaGuangzhou Zhiyuan Agricultural Technology Development Co., Ltd., Guangzhou 510507, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaCollege of Horticulture, South China Agricultural University, Guangzhou 510642, ChinaThe optimization of micropropagation for blueberries is crucial due to the growing blueberry industry and demand for plantlets. This study focused on four stages: explant sterilization, in vitro establishment, in vitro proliferation, and ex vitro rooting, aiming to establish an efficient in vitro propagation system for southern highbush blueberry cultivar ‘ZY09’. The most effective explant sterilization method was a 60 s treatment with 75% ethanol and a 5 min treatment with 4% NaClO. During the establishment of the in vitro culture, the modified woody plant medium was found to be suitable. The replacement of NH<sub>4</sub>NO<sub>3</sub> in woody plant medium with (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> facilitated the proliferation of blueberry microshoots. The optimal combination of plant growth regulators for the in vitro proliferation of blueberry microshoots was indole-3-butyric acid (0.1 mg·L<sup>−1</sup>), thidiazuron (0.0005 mg·L<sup>−1</sup>), and zeatin (1 mg·L<sup>−1</sup>). Perlite was the most suitable substrate for ex vitro rooting. The best ex vitro rooting performance was observed without immersion in growth regulators. Indole-3-butyric acid enhances root formation and suppresses root elongation in blueberries. The findings of this study can be applied to large-scale in vitro propagation of southern highbush blueberry and provide a reference for the genetic transformation of blueberries.https://www.mdpi.com/2311-7524/9/8/893blueberrymicropropagationwoody plant medium(NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>growth regulatorsex vitro rooting |
| spellingShingle | Yuting Wang Xiaoyun Zhang Zhehao Jiang Xiaolong Yang Xiaojuan Liu Xi Ou Wei Su Riyuan Chen Establishment and Optimization of Micropropagation System for Southern Highbush Blueberry Horticulturae blueberry micropropagation woody plant medium (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> growth regulators ex vitro rooting |
| title | Establishment and Optimization of Micropropagation System for Southern Highbush Blueberry |
| title_full | Establishment and Optimization of Micropropagation System for Southern Highbush Blueberry |
| title_fullStr | Establishment and Optimization of Micropropagation System for Southern Highbush Blueberry |
| title_full_unstemmed | Establishment and Optimization of Micropropagation System for Southern Highbush Blueberry |
| title_short | Establishment and Optimization of Micropropagation System for Southern Highbush Blueberry |
| title_sort | establishment and optimization of micropropagation system for southern highbush blueberry |
| topic | blueberry micropropagation woody plant medium (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> growth regulators ex vitro rooting |
| url | https://www.mdpi.com/2311-7524/9/8/893 |
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