Starvation causes female-to-male sex reversal through lipid metabolism in the teleost fish, medaka (Olyzias latipes)
The teleost fish, medaka (Oryzias latipes), employs the XX/XY genetic sex determination system. We show here that the phenotypic sex of medaka is affected by changes in lipid metabolism. Medaka larvae subjected to 5 days of starvation underwent female-to-male sex reversal. Metabolomic and RT-qPCR an...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2020-04-01
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| Series: | Biology Open |
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| Online Access: | http://bio.biologists.org/content/9/4/bio050054 |
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| author | Yuta Sakae Akira Oikawa Yuki Sugiura Masatoshi Mita Shuhei Nakamura Toshiya Nishimura Makoto Suematsu Minoru Tanaka |
| author_facet | Yuta Sakae Akira Oikawa Yuki Sugiura Masatoshi Mita Shuhei Nakamura Toshiya Nishimura Makoto Suematsu Minoru Tanaka |
| author_sort | Yuta Sakae |
| collection | DOAJ |
| description | The teleost fish, medaka (Oryzias latipes), employs the XX/XY genetic sex determination system. We show here that the phenotypic sex of medaka is affected by changes in lipid metabolism. Medaka larvae subjected to 5 days of starvation underwent female-to-male sex reversal. Metabolomic and RT-qPCR analyses indicated that pantothenate metabolism was suppressed by starvation. Consistently, inhibiting the pantothenate metabolic pathway caused sex reversal. The final metabolite in this pathway is coenzyme A, an essential factor for lipogenesis. Inhibiting fatty acid synthesis, the first step of lipogenesis, also caused sex reversal. The expression of dmrt1, a critical gene for male development, was suppressed by starvation, and a dmrt1 (Δ13) mutant did not show sex reversal under starvation. Collectively, these results indicate that fatty acid synthesis is involved in female-to-male sex reversal through ectopic expression of male gene dmrt1 under starvation. |
| first_indexed | 2024-12-21T18:26:59Z |
| format | Article |
| id | doaj.art-1b3e1f7aa7974ff095e0af4bc478e26d |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-21T18:26:59Z |
| publishDate | 2020-04-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-1b3e1f7aa7974ff095e0af4bc478e26d2022-12-21T18:54:22ZengThe Company of BiologistsBiology Open2046-63902020-04-019410.1242/bio.050054050054Starvation causes female-to-male sex reversal through lipid metabolism in the teleost fish, medaka (Olyzias latipes)Yuta Sakae0Akira Oikawa1Yuki Sugiura2Masatoshi Mita3Shuhei Nakamura4Toshiya Nishimura5Makoto Suematsu6Minoru Tanaka7 Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan RIKEN Center for Sustainable Resource Science, Metabolomics Research Group, Yokohama 230-0045, Japan Department of Biochemistry, Keio University School of Medicine, Tokyo 160-8582, Japan Department of Biochemistry, Showa University School of Medicine, Tokyo 142-8555, Japan Institute for Advanced Co-Creation Studies, Osaka University, Osaka 565-0871, Japan Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan Department of Biochemistry, Keio University School of Medicine, Tokyo 160-8582, Japan Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan The teleost fish, medaka (Oryzias latipes), employs the XX/XY genetic sex determination system. We show here that the phenotypic sex of medaka is affected by changes in lipid metabolism. Medaka larvae subjected to 5 days of starvation underwent female-to-male sex reversal. Metabolomic and RT-qPCR analyses indicated that pantothenate metabolism was suppressed by starvation. Consistently, inhibiting the pantothenate metabolic pathway caused sex reversal. The final metabolite in this pathway is coenzyme A, an essential factor for lipogenesis. Inhibiting fatty acid synthesis, the first step of lipogenesis, also caused sex reversal. The expression of dmrt1, a critical gene for male development, was suppressed by starvation, and a dmrt1 (Δ13) mutant did not show sex reversal under starvation. Collectively, these results indicate that fatty acid synthesis is involved in female-to-male sex reversal through ectopic expression of male gene dmrt1 under starvation.http://bio.biologists.org/content/9/4/bio050054sex reversalmetabolomepantothenate metabolismfatty acid synthesis |
| spellingShingle | Yuta Sakae Akira Oikawa Yuki Sugiura Masatoshi Mita Shuhei Nakamura Toshiya Nishimura Makoto Suematsu Minoru Tanaka Starvation causes female-to-male sex reversal through lipid metabolism in the teleost fish, medaka (Olyzias latipes) Biology Open sex reversal metabolome pantothenate metabolism fatty acid synthesis |
| title | Starvation causes female-to-male sex reversal through lipid metabolism in the teleost fish, medaka (Olyzias latipes) |
| title_full | Starvation causes female-to-male sex reversal through lipid metabolism in the teleost fish, medaka (Olyzias latipes) |
| title_fullStr | Starvation causes female-to-male sex reversal through lipid metabolism in the teleost fish, medaka (Olyzias latipes) |
| title_full_unstemmed | Starvation causes female-to-male sex reversal through lipid metabolism in the teleost fish, medaka (Olyzias latipes) |
| title_short | Starvation causes female-to-male sex reversal through lipid metabolism in the teleost fish, medaka (Olyzias latipes) |
| title_sort | starvation causes female to male sex reversal through lipid metabolism in the teleost fish medaka olyzias latipes |
| topic | sex reversal metabolome pantothenate metabolism fatty acid synthesis |
| url | http://bio.biologists.org/content/9/4/bio050054 |
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