Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from <i>Panax Ginseng</i> C. A. Meyer
<i>Panax ginseng</i> (<i>P. ginseng</i>) is the most widely consumed herbal plant in Asia and is well-known for its various pharmacological properties. Many studies have been devoted to this natural product. However, polysaccharide’s components of ginseng and their...
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MDPI AG
2020-02-01
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| author | Hyung Min Kim Yanxue Song Gyu Hwan Hyun Nguyen Phuoc Long Jeong Hill Park Yves S.Y. Hsieh Sung Won Kwon |
| author_facet | Hyung Min Kim Yanxue Song Gyu Hwan Hyun Nguyen Phuoc Long Jeong Hill Park Yves S.Y. Hsieh Sung Won Kwon |
| author_sort | Hyung Min Kim |
| collection | DOAJ |
| description | <i>Panax ginseng</i> (<i>P. ginseng</i>) is the most widely consumed herbal plant in Asia and is well-known for its various pharmacological properties. Many studies have been devoted to this natural product. However, polysaccharide’s components of ginseng and their biological effects have not been widely studied. In this study, white ginseng neutral polysaccharide (WGNP) and white ginseng acidic polysaccharide (WGAP) fractions were purified from <i>P. ginseng</i> roots. The chemical properties of WGNP and WGAP were investigated using various chromatography and spectroscopy techniques, including high-performance gel permeation chromatography, Fourier-transform infrared spectroscopy, and high-performance liquid chromatography with an ultra-violet detector. The antioxidant, anti-radical, and hydrogen peroxide scavenging activities were evaluated in vitro and in vivo using <i>Caenorhabditis elegans</i> as the model organism. Our in vitro data by ABTS (2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), reducing power, ferrous ion chelating, and hydroxyl radical scavenging activity suggested that the WGAP with significantly higher uronic acid content and higher molecular weight exhibits a much stronger antioxidant effect as compared to that of WGNP. Similar antioxidant activity of WGAP was also confirmed in vivo by evaluating internal reactive oxygen species (ROS) concentration and lipid peroxidation. In conclusion, WGAP may be used as a natural antioxidant with potent scavenging and metal chelation properties. |
| first_indexed | 2024-12-10T16:30:00Z |
| format | Article |
| id | doaj.art-1b3f5b5ae6714d0a8bdacad3c18d872a |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T16:30:00Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1b3f5b5ae6714d0a8bdacad3c18d872a2022-12-22T01:41:33ZengMDPI AGMolecules1420-30492020-02-0125479110.3390/molecules25040791molecules25040791Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from <i>Panax Ginseng</i> C. A. MeyerHyung Min Kim0Yanxue Song1Gyu Hwan Hyun2Nguyen Phuoc Long3Jeong Hill Park4Yves S.Y. Hsieh5Sung Won Kwon6College of Pharmacy, Seoul National University, Seoul 08826, KoreaCollege of Pharmacy, Seoul National University, Seoul 08826, KoreaCollege of Pharmacy, Seoul National University, Seoul 08826, KoreaCollege of Pharmacy, Seoul National University, Seoul 08826, KoreaCollege of Pharmacy, Seoul National University, Seoul 08826, KoreaDivision of Glycoscience, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, Royal Institute of Technology (KTH), SE-106 91 Stockholm, SwedenCollege of Pharmacy, Seoul National University, Seoul 08826, Korea<i>Panax ginseng</i> (<i>P. ginseng</i>) is the most widely consumed herbal plant in Asia and is well-known for its various pharmacological properties. Many studies have been devoted to this natural product. However, polysaccharide’s components of ginseng and their biological effects have not been widely studied. In this study, white ginseng neutral polysaccharide (WGNP) and white ginseng acidic polysaccharide (WGAP) fractions were purified from <i>P. ginseng</i> roots. The chemical properties of WGNP and WGAP were investigated using various chromatography and spectroscopy techniques, including high-performance gel permeation chromatography, Fourier-transform infrared spectroscopy, and high-performance liquid chromatography with an ultra-violet detector. The antioxidant, anti-radical, and hydrogen peroxide scavenging activities were evaluated in vitro and in vivo using <i>Caenorhabditis elegans</i> as the model organism. Our in vitro data by ABTS (2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), reducing power, ferrous ion chelating, and hydroxyl radical scavenging activity suggested that the WGAP with significantly higher uronic acid content and higher molecular weight exhibits a much stronger antioxidant effect as compared to that of WGNP. Similar antioxidant activity of WGAP was also confirmed in vivo by evaluating internal reactive oxygen species (ROS) concentration and lipid peroxidation. In conclusion, WGAP may be used as a natural antioxidant with potent scavenging and metal chelation properties.https://www.mdpi.com/1420-3049/25/4/791<i>panax ginseng</i>acidic polysaccharideneutral polysaccharide<i>caenorhabditis elegans</i>antioxidant effect |
| spellingShingle | Hyung Min Kim Yanxue Song Gyu Hwan Hyun Nguyen Phuoc Long Jeong Hill Park Yves S.Y. Hsieh Sung Won Kwon Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from <i>Panax Ginseng</i> C. A. Meyer Molecules <i>panax ginseng</i> acidic polysaccharide neutral polysaccharide <i>caenorhabditis elegans</i> antioxidant effect |
| title | Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from <i>Panax Ginseng</i> C. A. Meyer |
| title_full | Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from <i>Panax Ginseng</i> C. A. Meyer |
| title_fullStr | Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from <i>Panax Ginseng</i> C. A. Meyer |
| title_full_unstemmed | Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from <i>Panax Ginseng</i> C. A. Meyer |
| title_short | Characterization and Antioxidant Activity Determination of Neutral and Acidic Polysaccharides from <i>Panax Ginseng</i> C. A. Meyer |
| title_sort | characterization and antioxidant activity determination of neutral and acidic polysaccharides from i panax ginseng i c a meyer |
| topic | <i>panax ginseng</i> acidic polysaccharide neutral polysaccharide <i>caenorhabditis elegans</i> antioxidant effect |
| url | https://www.mdpi.com/1420-3049/25/4/791 |
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