Hepatic transcriptome analyses of juvenile white bass (Morone chrysops) when fed diets where fish meal is partially or totally replaced by alternative protein sources
White bass (Morone chrysops) are a popular sportfish throughout the southern United States, and one parent of the commercially-successful hybrid striped bass (M. chrysops ♂ x M. saxatilis ♀). Currently, white bass are cultured using diets formulated for other carnivorous fish, such as largemouth bas...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1308690/full |
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| author | S. Adam Fuller Jason W. Abernathy Nithin Muliya Sankappa Nithin Muliya Sankappa Benjamin H. Beck Steven D. Rawles Bartholomew W. Green Kurt A. Rosentrater Matthew E. McEntire George Huskey Carl D. Webster |
| author_facet | S. Adam Fuller Jason W. Abernathy Nithin Muliya Sankappa Nithin Muliya Sankappa Benjamin H. Beck Steven D. Rawles Bartholomew W. Green Kurt A. Rosentrater Matthew E. McEntire George Huskey Carl D. Webster |
| author_sort | S. Adam Fuller |
| collection | DOAJ |
| description | White bass (Morone chrysops) are a popular sportfish throughout the southern United States, and one parent of the commercially-successful hybrid striped bass (M. chrysops ♂ x M. saxatilis ♀). Currently, white bass are cultured using diets formulated for other carnivorous fish, such as largemouth bass (Micropterus salmoides) or hybrid striped bass and contain a significant percentage of marine fish meal. Since there are no studies regarding the utilization of alternative proteins in this species, we evaluated the global gene expression of white bass fed diets in which fish meal was partially or totally replaced by various combinations of soybean meal, poultry by-product meal, canola meal, soy protein concentrate, wheat gluten, or a commercial protein blend (Pro-Cision™). Six isonitrogenous (40% protein), isolipidic (11%), and isocaloric (17.1 kJ/g) diets were formulated to meet the known nutrient and energy requirements of largemouth bass and hybrid striped bass using nutrient availability data for most of the dietary ingredients. One of the test diets consisted exclusively of plant protein sources. Juvenile white bass (40.2 g initial weight) were stocked into a flow-through aquaculture system (three tanks/diet; 10 fish/tank) and fed the test diets twice daily to satiation for 60 days. RNA sequencing and bioinformatic analyses revealed significant differentially expressed genes between all test diets when compared to fish meal control. A total of 1,260 differentially expressed genes were identified, with major ontology relating to cell cycle and metabolic processes as well as immune gene functions. This data will be useful as a resource for future refinements to moronid diet formulation, as marine fish meal becomes limiting and plant ingredients are increasingly added as a reliable protein source. |
| first_indexed | 2024-03-08T14:08:41Z |
| format | Article |
| id | doaj.art-1b3fa5dbf50a49cdb520410abaa8f475 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-03-08T14:08:41Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-1b3fa5dbf50a49cdb520410abaa8f4752024-01-15T04:20:19ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2024-01-011410.3389/fphys.2023.13086901308690Hepatic transcriptome analyses of juvenile white bass (Morone chrysops) when fed diets where fish meal is partially or totally replaced by alternative protein sourcesS. Adam Fuller0Jason W. Abernathy1Nithin Muliya Sankappa2Nithin Muliya Sankappa3Benjamin H. Beck4Steven D. Rawles5Bartholomew W. Green6Kurt A. Rosentrater7Matthew E. McEntire8George Huskey9Carl D. Webster10USDA-ARS Harry K. Dupree Stuttgart National Aquaculture Research Center (HKDSNARC), Stuttgart, AR, United StatesUSDA-ARS Aquatic Animal Health Research Unit (AAHRU), Auburn, AL, United StatesUSDA-ARS Aquatic Animal Health Research Unit (AAHRU), Auburn, AL, United StatesOak Ridge Institute for Science and Education (ORISE), ARS Research Participation Program, Oak Ridge, TN, United StatesUSDA-ARS Aquatic Animal Health Research Unit (AAHRU), Auburn, AL, United StatesUSDA-ARS Harry K. Dupree Stuttgart National Aquaculture Research Center (HKDSNARC), Stuttgart, AR, United StatesUSDA-ARS Harry K. Dupree Stuttgart National Aquaculture Research Center (HKDSNARC), Stuttgart, AR, United StatesIowa State University, Agricultural and Biosystems Engineering, Ames, IA, United StatesUSDA-ARS Harry K. Dupree Stuttgart National Aquaculture Research Center (HKDSNARC), Stuttgart, AR, United StatesUSDA-ARS Harry K. Dupree Stuttgart National Aquaculture Research Center (HKDSNARC), Stuttgart, AR, United StatesUSDA-ARS Harry K. Dupree Stuttgart National Aquaculture Research Center (HKDSNARC), Stuttgart, AR, United StatesWhite bass (Morone chrysops) are a popular sportfish throughout the southern United States, and one parent of the commercially-successful hybrid striped bass (M. chrysops ♂ x M. saxatilis ♀). Currently, white bass are cultured using diets formulated for other carnivorous fish, such as largemouth bass (Micropterus salmoides) or hybrid striped bass and contain a significant percentage of marine fish meal. Since there are no studies regarding the utilization of alternative proteins in this species, we evaluated the global gene expression of white bass fed diets in which fish meal was partially or totally replaced by various combinations of soybean meal, poultry by-product meal, canola meal, soy protein concentrate, wheat gluten, or a commercial protein blend (Pro-Cision™). Six isonitrogenous (40% protein), isolipidic (11%), and isocaloric (17.1 kJ/g) diets were formulated to meet the known nutrient and energy requirements of largemouth bass and hybrid striped bass using nutrient availability data for most of the dietary ingredients. One of the test diets consisted exclusively of plant protein sources. Juvenile white bass (40.2 g initial weight) were stocked into a flow-through aquaculture system (three tanks/diet; 10 fish/tank) and fed the test diets twice daily to satiation for 60 days. RNA sequencing and bioinformatic analyses revealed significant differentially expressed genes between all test diets when compared to fish meal control. A total of 1,260 differentially expressed genes were identified, with major ontology relating to cell cycle and metabolic processes as well as immune gene functions. This data will be useful as a resource for future refinements to moronid diet formulation, as marine fish meal becomes limiting and plant ingredients are increasingly added as a reliable protein source.https://www.frontiersin.org/articles/10.3389/fphys.2023.1308690/fullalternative dietscarnivorous fishfish mealplant mealtranscriptomicsRNAseq |
| spellingShingle | S. Adam Fuller Jason W. Abernathy Nithin Muliya Sankappa Nithin Muliya Sankappa Benjamin H. Beck Steven D. Rawles Bartholomew W. Green Kurt A. Rosentrater Matthew E. McEntire George Huskey Carl D. Webster Hepatic transcriptome analyses of juvenile white bass (Morone chrysops) when fed diets where fish meal is partially or totally replaced by alternative protein sources Frontiers in Physiology alternative diets carnivorous fish fish meal plant meal transcriptomics RNAseq |
| title | Hepatic transcriptome analyses of juvenile white bass (Morone chrysops) when fed diets where fish meal is partially or totally replaced by alternative protein sources |
| title_full | Hepatic transcriptome analyses of juvenile white bass (Morone chrysops) when fed diets where fish meal is partially or totally replaced by alternative protein sources |
| title_fullStr | Hepatic transcriptome analyses of juvenile white bass (Morone chrysops) when fed diets where fish meal is partially or totally replaced by alternative protein sources |
| title_full_unstemmed | Hepatic transcriptome analyses of juvenile white bass (Morone chrysops) when fed diets where fish meal is partially or totally replaced by alternative protein sources |
| title_short | Hepatic transcriptome analyses of juvenile white bass (Morone chrysops) when fed diets where fish meal is partially or totally replaced by alternative protein sources |
| title_sort | hepatic transcriptome analyses of juvenile white bass morone chrysops when fed diets where fish meal is partially or totally replaced by alternative protein sources |
| topic | alternative diets carnivorous fish fish meal plant meal transcriptomics RNAseq |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1308690/full |
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