In vivo calcium imaging reveals disordered interictal network dynamics in epileptic stxbp1b zebrafish
Summary: STXBP1 mutations are associated with encephalopathy, developmental delay, intellectual disability, and epilepsy. While neural networks are known to operate at a critical state in the healthy brain, network behavior during pathological epileptic states remains unclear. Examining activity dur...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-06-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221005265 |
| _version_ | 1819083557126012928 |
|---|---|
| author | Jing Liu Kathryn A. Salvati Scott C. Baraban |
| author_facet | Jing Liu Kathryn A. Salvati Scott C. Baraban |
| author_sort | Jing Liu |
| collection | DOAJ |
| description | Summary: STXBP1 mutations are associated with encephalopathy, developmental delay, intellectual disability, and epilepsy. While neural networks are known to operate at a critical state in the healthy brain, network behavior during pathological epileptic states remains unclear. Examining activity during periods between well-characterized ictal-like events (i.e., interictal period) could provide a valuable step toward understanding epileptic networks. To study these networks in the context of STXBP1 mutations, we combine a larval zebrafish model with in vivo fast confocal calcium imaging and extracellular local field potential recordings. Stxbp1b mutants display transient periods of elevated activity among local clusters of interacting neurons. These network “cascade” events were significantly larger in size and duration in mutants. At mesoscale resolution, cascades exhibit neurodevelopmental abnormalities. At single-cell scale, we describe spontaneous hyper-synchronized neuronal ensembles. That calcium imaging reveals uniquely disordered brain states during periods between pathological ictal-like seizure events is striking and represents a potential interictal biomarker. |
| first_indexed | 2024-12-21T20:34:27Z |
| format | Article |
| id | doaj.art-1b3fd895552e4e51ab633cc03b8ad321 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-21T20:34:27Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-1b3fd895552e4e51ab633cc03b8ad3212022-12-21T18:51:08ZengElsevieriScience2589-00422021-06-01246102558In vivo calcium imaging reveals disordered interictal network dynamics in epileptic stxbp1b zebrafishJing Liu0Kathryn A. Salvati1Scott C. Baraban2Epilepsy Research Laboratory and Weill Institute for Neuroscience, Department of Neurological Surgery, University of California San Francisco, San Francisco, CA 94122, USAEpilepsy Research Laboratory and Weill Institute for Neuroscience, Department of Neurological Surgery, University of California San Francisco, San Francisco, CA 94122, USAEpilepsy Research Laboratory and Weill Institute for Neuroscience, Department of Neurological Surgery, University of California San Francisco, San Francisco, CA 94122, USA; Corresponding authorSummary: STXBP1 mutations are associated with encephalopathy, developmental delay, intellectual disability, and epilepsy. While neural networks are known to operate at a critical state in the healthy brain, network behavior during pathological epileptic states remains unclear. Examining activity during periods between well-characterized ictal-like events (i.e., interictal period) could provide a valuable step toward understanding epileptic networks. To study these networks in the context of STXBP1 mutations, we combine a larval zebrafish model with in vivo fast confocal calcium imaging and extracellular local field potential recordings. Stxbp1b mutants display transient periods of elevated activity among local clusters of interacting neurons. These network “cascade” events were significantly larger in size and duration in mutants. At mesoscale resolution, cascades exhibit neurodevelopmental abnormalities. At single-cell scale, we describe spontaneous hyper-synchronized neuronal ensembles. That calcium imaging reveals uniquely disordered brain states during periods between pathological ictal-like seizure events is striking and represents a potential interictal biomarker.http://www.sciencedirect.com/science/article/pii/S2589004221005265Optical imagingMolecular neuroscienceCellular neuroscience |
| spellingShingle | Jing Liu Kathryn A. Salvati Scott C. Baraban In vivo calcium imaging reveals disordered interictal network dynamics in epileptic stxbp1b zebrafish iScience Optical imaging Molecular neuroscience Cellular neuroscience |
| title | In vivo calcium imaging reveals disordered interictal network dynamics in epileptic stxbp1b zebrafish |
| title_full | In vivo calcium imaging reveals disordered interictal network dynamics in epileptic stxbp1b zebrafish |
| title_fullStr | In vivo calcium imaging reveals disordered interictal network dynamics in epileptic stxbp1b zebrafish |
| title_full_unstemmed | In vivo calcium imaging reveals disordered interictal network dynamics in epileptic stxbp1b zebrafish |
| title_short | In vivo calcium imaging reveals disordered interictal network dynamics in epileptic stxbp1b zebrafish |
| title_sort | in vivo calcium imaging reveals disordered interictal network dynamics in epileptic stxbp1b zebrafish |
| topic | Optical imaging Molecular neuroscience Cellular neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221005265 |
| work_keys_str_mv | AT jingliu invivocalciumimagingrevealsdisorderedinterictalnetworkdynamicsinepilepticstxbp1bzebrafish AT kathrynasalvati invivocalciumimagingrevealsdisorderedinterictalnetworkdynamicsinepilepticstxbp1bzebrafish AT scottcbaraban invivocalciumimagingrevealsdisorderedinterictalnetworkdynamicsinepilepticstxbp1bzebrafish |