MICROPROPAGATION AND START CODON TARGETED CHARACTERIZATION OF FOUR STEVIA CULTIVARS IN EGYPT
calorie crop and commercially used as a non-caloric sweetener for diabetic patients. It is also used as cosmetic ingredient, pickling agent, and dentifrice. Four cultivars (Spantia, Shou2A3, China, and High Sugar) of stevia were included to optimize in vitro micropropagation. Four different combinat...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Arabic |
| Published: |
The Union of Arab Universities
2018-09-01
|
| Series: | Arab Universities Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://ajs.journals.ekb.eg/article_15968_af7b54b25f29008eb3fe9695cfe48aef.pdf |
| _version_ | 1797269716145799168 |
|---|---|
| author | Reham Abd ElHamid F. Abd ElTawab A. Abdel Razik A. Allam A. ELDoliefy |
| author_facet | Reham Abd ElHamid F. Abd ElTawab A. Abdel Razik A. Allam A. ELDoliefy |
| author_sort | Reham Abd ElHamid |
| collection | DOAJ |
| description | calorie crop and commercially used as a non-caloric sweetener for diabetic patients. It is also used as cosmetic ingredient, pickling agent, and dentifrice. Four cultivars (Spantia, Shou2A3, China, and High Sugar) of stevia were included to optimize in vitro micropropagation. Four different combinations of hormonal treatments were investigated [6-benzylamino purine (BAP) + Kinetin (Kin) (0.25 + 0.25 mg/l); Forchloefenuron (Cppu) + Kin (0.25 + 0.25 mg/l); Cppu+ Kin (0.5+0.25 mg/l); and the control medium (hormone-free)]. Out of the different media components, the hormone-free medium produced the best performance of explants. The analysis of variance showed that the control treatment was the most significant for all traits except the number of branches per cutting. Hardening of rooted plants was performed in plastic pots with 70% survival percentage during acclimatization. Molecular characterization, of the four stevia cultivars, was conducted using 11 SCoT primers. The SCoT analysis resulted in 122 amplicons, of which, 62 amplicons (51%) were polymorphic. The range of polymorphism was between 6 % and 91 %. The range of polymorphic amplicons per primer was between one and 12 amplicons. The SCoT-16 produced the highest number of polymorphic bands (12). Meanwhile, the SCoT-24 produced the least polymorphism (6 %). The current study provides a new micropropagation system with low cost, high efficiency, and hormone-free application. Additionally, the study provides the first molecular characterization of stevia using SCoT marker system. Finally, SCoT markers associated with cultivars having high and low contents of stevioside can further be validated by marker-assisted breeding studies. |
| first_indexed | 2024-04-25T01:52:47Z |
| format | Article |
| id | doaj.art-1b4251799b764576b1ca5db11c714106 |
| institution | Directory Open Access Journal |
| issn | 1110-2675 2636-3585 |
| language | Arabic |
| last_indexed | 2024-04-25T01:52:47Z |
| publishDate | 2018-09-01 |
| publisher | The Union of Arab Universities |
| record_format | Article |
| series | Arab Universities Journal of Agricultural Sciences |
| spelling | doaj.art-1b4251799b764576b1ca5db11c7141062024-03-07T17:39:14ZaraThe Union of Arab UniversitiesArab Universities Journal of Agricultural Sciences1110-26752636-35852018-09-0126263564510.21608/ajs.2018.1596815968MICROPROPAGATION AND START CODON TARGETED CHARACTERIZATION OF FOUR STEVIA CULTIVARS IN EGYPTReham Abd ElHamid0F. Abd ElTawab1A. Abdel Razik2A. Allam3A. ELDoliefy4Tissue culture Dept., Sugar Crops Research Institute, Agric. Research Center (ARC), Giza, EgyptGenetics Dept., Fac. of Agric., Ain Shams Univ., Cairo, EgyptGenetics Dept., Fac. of Agric., Ain Shams Univ., Cairo, EgyptTissue culture Dept., Sugar Crops Research Institute, Agric. Research Center (ARC), Giza, EgyptPlant Molecular Biology Dept., Agric. Genetic Engineering Research Institute, Giza, Egyptcalorie crop and commercially used as a non-caloric sweetener for diabetic patients. It is also used as cosmetic ingredient, pickling agent, and dentifrice. Four cultivars (Spantia, Shou2A3, China, and High Sugar) of stevia were included to optimize in vitro micropropagation. Four different combinations of hormonal treatments were investigated [6-benzylamino purine (BAP) + Kinetin (Kin) (0.25 + 0.25 mg/l); Forchloefenuron (Cppu) + Kin (0.25 + 0.25 mg/l); Cppu+ Kin (0.5+0.25 mg/l); and the control medium (hormone-free)]. Out of the different media components, the hormone-free medium produced the best performance of explants. The analysis of variance showed that the control treatment was the most significant for all traits except the number of branches per cutting. Hardening of rooted plants was performed in plastic pots with 70% survival percentage during acclimatization. Molecular characterization, of the four stevia cultivars, was conducted using 11 SCoT primers. The SCoT analysis resulted in 122 amplicons, of which, 62 amplicons (51%) were polymorphic. The range of polymorphism was between 6 % and 91 %. The range of polymorphic amplicons per primer was between one and 12 amplicons. The SCoT-16 produced the highest number of polymorphic bands (12). Meanwhile, the SCoT-24 produced the least polymorphism (6 %). The current study provides a new micropropagation system with low cost, high efficiency, and hormone-free application. Additionally, the study provides the first molecular characterization of stevia using SCoT marker system. Finally, SCoT markers associated with cultivars having high and low contents of stevioside can further be validated by marker-assisted breeding studies.https://ajs.journals.ekb.eg/article_15968_af7b54b25f29008eb3fe9695cfe48aef.pdfms mediumnon-caloric sweetenerstevia rebaudianamicropropagationscot markercppukinbap |
| spellingShingle | Reham Abd ElHamid F. Abd ElTawab A. Abdel Razik A. Allam A. ELDoliefy MICROPROPAGATION AND START CODON TARGETED CHARACTERIZATION OF FOUR STEVIA CULTIVARS IN EGYPT Arab Universities Journal of Agricultural Sciences ms medium non-caloric sweetener stevia rebaudiana micropropagation scot marker cppu kin bap |
| title | MICROPROPAGATION AND START CODON TARGETED CHARACTERIZATION OF FOUR STEVIA CULTIVARS IN EGYPT |
| title_full | MICROPROPAGATION AND START CODON TARGETED CHARACTERIZATION OF FOUR STEVIA CULTIVARS IN EGYPT |
| title_fullStr | MICROPROPAGATION AND START CODON TARGETED CHARACTERIZATION OF FOUR STEVIA CULTIVARS IN EGYPT |
| title_full_unstemmed | MICROPROPAGATION AND START CODON TARGETED CHARACTERIZATION OF FOUR STEVIA CULTIVARS IN EGYPT |
| title_short | MICROPROPAGATION AND START CODON TARGETED CHARACTERIZATION OF FOUR STEVIA CULTIVARS IN EGYPT |
| title_sort | micropropagation and start codon targeted characterization of four stevia cultivars in egypt |
| topic | ms medium non-caloric sweetener stevia rebaudiana micropropagation scot marker cppu kin bap |
| url | https://ajs.journals.ekb.eg/article_15968_af7b54b25f29008eb3fe9695cfe48aef.pdf |
| work_keys_str_mv | AT rehamabdelhamid micropropagationandstartcodontargetedcharacterizationoffoursteviacultivarsinegypt AT fabdeltawab micropropagationandstartcodontargetedcharacterizationoffoursteviacultivarsinegypt AT aabdelrazik micropropagationandstartcodontargetedcharacterizationoffoursteviacultivarsinegypt AT aallam micropropagationandstartcodontargetedcharacterizationoffoursteviacultivarsinegypt AT aeldoliefy micropropagationandstartcodontargetedcharacterizationoffoursteviacultivarsinegypt |