A recombinase polymerase amplification–based assay for rapid detection of Chlamydia psittaci
Chlamydia psittaci is a zoonotic agent of systemic wasting disease in birds and atypical pneumonia in mammalians including humans, constituting a public health risk. A rapid diagnostic assay would be beneficial in screening C. psittaci in the field. In this study, we developed a probe-based recombin...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-02-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S003257912030883X |
| _version_ | 1819296190300160000 |
|---|---|
| author | Yanling Pang Feng Cong Xinheng Zhang Hongxin Li Yung-Fu Chang Qingmei Xie Wencheng Lin |
| author_facet | Yanling Pang Feng Cong Xinheng Zhang Hongxin Li Yung-Fu Chang Qingmei Xie Wencheng Lin |
| author_sort | Yanling Pang |
| collection | DOAJ |
| description | Chlamydia psittaci is a zoonotic agent of systemic wasting disease in birds and atypical pneumonia in mammalians including humans, constituting a public health risk. A rapid diagnostic assay would be beneficial in screening C. psittaci in the field. In this study, we developed a probe-based recombinase polymerase amplification (RPA) assay for the rapid detection of C. psittaci. The specific primer pairs and probe targeting the conserved region of the outer membrane protein A gene were designed and applied to the real-time real-time RPA assay. The test can be performed at 39°C for 20 min using a portable device, with sensitivities approaching 100 copies of DNA molecules per reaction, with no cross-reaction with other pathogens. The clinical performance of the RPA assay was evaluated in an outbreak of C. psittaci and has high accuracy levels in field applications. The epidemic C. psittaci strains were classed into 2 genotypes: A and C. Collectively, this study offers a promising approach in screening for C. psittaci both in a laboratory setting and in field settings, and RPA can be used as an effective clinical test to monitor outbreaks in domestic fowl populations. |
| first_indexed | 2024-12-24T04:54:10Z |
| format | Article |
| id | doaj.art-1b4410b7a07e4021a7ec507a1d7cc2fb |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-24T04:54:10Z |
| publishDate | 2021-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-1b4410b7a07e4021a7ec507a1d7cc2fb2022-12-21T17:14:27ZengElsevierPoultry Science0032-57912021-02-011002585591A recombinase polymerase amplification–based assay for rapid detection of Chlamydia psittaciYanling Pang0Feng Cong1Xinheng Zhang2Hongxin Li3Yung-Fu Chang4Qingmei Xie5Wencheng Lin6Guangdong Provincial Animal Virus Vector Vaccine Engineering Technology Research Center, College of Animal Science, South China Agricultural University, Guangzhou 510642, P.R. ChinaGuangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute, Guangzhou 510633, P.R. ChinaGuangdong Provincial Animal Virus Vector Vaccine Engineering Technology Research Center, College of Animal Science, South China Agricultural University, Guangzhou 510642, P.R. ChinaGuangdong Provincial Animal Virus Vector Vaccine Engineering Technology Research Center, College of Animal Science, South China Agricultural University, Guangzhou 510642, P.R. ChinaDepartment of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USAGuangdong Provincial Animal Virus Vector Vaccine Engineering Technology Research Center, College of Animal Science, South China Agricultural University, Guangzhou 510642, P.R. ChinaGuangdong Provincial Animal Virus Vector Vaccine Engineering Technology Research Center, College of Animal Science, South China Agricultural University, Guangzhou 510642, P.R. China; Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA; Corresponding author:Chlamydia psittaci is a zoonotic agent of systemic wasting disease in birds and atypical pneumonia in mammalians including humans, constituting a public health risk. A rapid diagnostic assay would be beneficial in screening C. psittaci in the field. In this study, we developed a probe-based recombinase polymerase amplification (RPA) assay for the rapid detection of C. psittaci. The specific primer pairs and probe targeting the conserved region of the outer membrane protein A gene were designed and applied to the real-time real-time RPA assay. The test can be performed at 39°C for 20 min using a portable device, with sensitivities approaching 100 copies of DNA molecules per reaction, with no cross-reaction with other pathogens. The clinical performance of the RPA assay was evaluated in an outbreak of C. psittaci and has high accuracy levels in field applications. The epidemic C. psittaci strains were classed into 2 genotypes: A and C. Collectively, this study offers a promising approach in screening for C. psittaci both in a laboratory setting and in field settings, and RPA can be used as an effective clinical test to monitor outbreaks in domestic fowl populations.http://www.sciencedirect.com/science/article/pii/S003257912030883XChlamydia psittacidetectionrecombinase polymerase amplification |
| spellingShingle | Yanling Pang Feng Cong Xinheng Zhang Hongxin Li Yung-Fu Chang Qingmei Xie Wencheng Lin A recombinase polymerase amplification–based assay for rapid detection of Chlamydia psittaci Poultry Science Chlamydia psittaci detection recombinase polymerase amplification |
| title | A recombinase polymerase amplification–based assay for rapid detection of Chlamydia psittaci |
| title_full | A recombinase polymerase amplification–based assay for rapid detection of Chlamydia psittaci |
| title_fullStr | A recombinase polymerase amplification–based assay for rapid detection of Chlamydia psittaci |
| title_full_unstemmed | A recombinase polymerase amplification–based assay for rapid detection of Chlamydia psittaci |
| title_short | A recombinase polymerase amplification–based assay for rapid detection of Chlamydia psittaci |
| title_sort | recombinase polymerase amplification based assay for rapid detection of chlamydia psittaci |
| topic | Chlamydia psittaci detection recombinase polymerase amplification |
| url | http://www.sciencedirect.com/science/article/pii/S003257912030883X |
| work_keys_str_mv | AT yanlingpang arecombinasepolymeraseamplificationbasedassayforrapiddetectionofchlamydiapsittaci AT fengcong arecombinasepolymeraseamplificationbasedassayforrapiddetectionofchlamydiapsittaci AT xinhengzhang arecombinasepolymeraseamplificationbasedassayforrapiddetectionofchlamydiapsittaci AT hongxinli arecombinasepolymeraseamplificationbasedassayforrapiddetectionofchlamydiapsittaci AT yungfuchang arecombinasepolymeraseamplificationbasedassayforrapiddetectionofchlamydiapsittaci AT qingmeixie arecombinasepolymeraseamplificationbasedassayforrapiddetectionofchlamydiapsittaci AT wenchenglin arecombinasepolymeraseamplificationbasedassayforrapiddetectionofchlamydiapsittaci AT yanlingpang recombinasepolymeraseamplificationbasedassayforrapiddetectionofchlamydiapsittaci AT fengcong recombinasepolymeraseamplificationbasedassayforrapiddetectionofchlamydiapsittaci AT xinhengzhang recombinasepolymeraseamplificationbasedassayforrapiddetectionofchlamydiapsittaci AT hongxinli recombinasepolymeraseamplificationbasedassayforrapiddetectionofchlamydiapsittaci AT yungfuchang recombinasepolymeraseamplificationbasedassayforrapiddetectionofchlamydiapsittaci AT qingmeixie recombinasepolymeraseamplificationbasedassayforrapiddetectionofchlamydiapsittaci AT wenchenglin recombinasepolymeraseamplificationbasedassayforrapiddetectionofchlamydiapsittaci |