Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications
Polyethylene Glycol Diacrylate (PEGDA) tissue scaffolds having a thickness higher than 1 mm and without the presence of nutrient conduit networks were shown to have limited applications in tissue engineering due to the inability of cells to adhere and migrate within the scaffold. The PEGDA scaffold...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-09-01
|
| Series: | Journal of Functional Biomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4983/8/3/39 |
| _version_ | 1798002545007788032 |
|---|---|
| author | Hari Kotturi Alaeddin Abuabed Haris Zafar Elaine Sawyer Bipin Pallipparambil Harsha Jamadagni Morshed Khandaker |
| author_facet | Hari Kotturi Alaeddin Abuabed Haris Zafar Elaine Sawyer Bipin Pallipparambil Harsha Jamadagni Morshed Khandaker |
| author_sort | Hari Kotturi |
| collection | DOAJ |
| description | Polyethylene Glycol Diacrylate (PEGDA) tissue scaffolds having a thickness higher than 1 mm and without the presence of nutrient conduit networks were shown to have limited applications in tissue engineering due to the inability of cells to adhere and migrate within the scaffold. The PEGDA scaffold has been coated with polycaprolactone (PCL) electrospun nanofiber (ENF) membrane on both sides to overcome these limitations, thereby creating a functional PEGDA-PCL scaffold. This study examined the physical, mechanical, and biological properties of the PEGDA and PEGDA-PCL scaffolds to determine the effect of PCL coating on PEGDA. The physical characterization of PEGDA-PCL samples demonstrated the effectiveness of combining PCL with a PEGDA scaffold to expand its applications in tissue engineering. This study also found a significant improvement of elasticity of PEGDA due to the addition of PCL layers. This study shows that PEGDA-PCL scaffolds absorb nutrients with time and can provide an ideal environment for the survival of cells. Furthermore, cell viability tests indicate that the cell adhered, proliferated, and migrated in the PEGDA-PCL scaffold. Therefore, PCL ENF coating has a positive influence on PEGDA scaffold. |
| first_indexed | 2024-04-11T11:53:48Z |
| format | Article |
| id | doaj.art-1b462dd2a47a4f6ea7bec66c642a3fed |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-04-11T11:53:48Z |
| publishDate | 2017-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-1b462dd2a47a4f6ea7bec66c642a3fed2022-12-22T04:25:14ZengMDPI AGJournal of Functional Biomaterials2079-49832017-09-01833910.3390/jfb8030039jfb8030039Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering ApplicationsHari Kotturi0Alaeddin Abuabed1Haris Zafar2Elaine Sawyer3Bipin Pallipparambil4Harsha Jamadagni5Morshed Khandaker6Department of Biology, University of Central Oklahoma, Edmond, OK 73034, USADepartment of Engineering & Physics, University of Central Oklahoma, Edmond, OK 73034, USADepartment of Biology, University of Central Oklahoma, Edmond, OK 73034, USADepartment of Biology, University of Central Oklahoma, Edmond, OK 73034, USADepartment of Engineering & Physics, University of Central Oklahoma, Edmond, OK 73034, USADepartment of Engineering & Physics, University of Central Oklahoma, Edmond, OK 73034, USADepartment of Engineering & Physics, University of Central Oklahoma, Edmond, OK 73034, USAPolyethylene Glycol Diacrylate (PEGDA) tissue scaffolds having a thickness higher than 1 mm and without the presence of nutrient conduit networks were shown to have limited applications in tissue engineering due to the inability of cells to adhere and migrate within the scaffold. The PEGDA scaffold has been coated with polycaprolactone (PCL) electrospun nanofiber (ENF) membrane on both sides to overcome these limitations, thereby creating a functional PEGDA-PCL scaffold. This study examined the physical, mechanical, and biological properties of the PEGDA and PEGDA-PCL scaffolds to determine the effect of PCL coating on PEGDA. The physical characterization of PEGDA-PCL samples demonstrated the effectiveness of combining PCL with a PEGDA scaffold to expand its applications in tissue engineering. This study also found a significant improvement of elasticity of PEGDA due to the addition of PCL layers. This study shows that PEGDA-PCL scaffolds absorb nutrients with time and can provide an ideal environment for the survival of cells. Furthermore, cell viability tests indicate that the cell adhered, proliferated, and migrated in the PEGDA-PCL scaffold. Therefore, PCL ENF coating has a positive influence on PEGDA scaffold.https://www.mdpi.com/2079-4983/8/3/39polyethylene glycol diacrylatepolycaprolactonescaffoldelectrospunnanofiber |
| spellingShingle | Hari Kotturi Alaeddin Abuabed Haris Zafar Elaine Sawyer Bipin Pallipparambil Harsha Jamadagni Morshed Khandaker Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications Journal of Functional Biomaterials polyethylene glycol diacrylate polycaprolactone scaffold electrospun nanofiber |
| title | Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications |
| title_full | Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications |
| title_fullStr | Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications |
| title_full_unstemmed | Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications |
| title_short | Evaluation of Polyethylene Glycol Diacrylate-Polycaprolactone Scaffolds for Tissue Engineering Applications |
| title_sort | evaluation of polyethylene glycol diacrylate polycaprolactone scaffolds for tissue engineering applications |
| topic | polyethylene glycol diacrylate polycaprolactone scaffold electrospun nanofiber |
| url | https://www.mdpi.com/2079-4983/8/3/39 |
| work_keys_str_mv | AT harikotturi evaluationofpolyethyleneglycoldiacrylatepolycaprolactonescaffoldsfortissueengineeringapplications AT alaeddinabuabed evaluationofpolyethyleneglycoldiacrylatepolycaprolactonescaffoldsfortissueengineeringapplications AT hariszafar evaluationofpolyethyleneglycoldiacrylatepolycaprolactonescaffoldsfortissueengineeringapplications AT elainesawyer evaluationofpolyethyleneglycoldiacrylatepolycaprolactonescaffoldsfortissueengineeringapplications AT bipinpallipparambil evaluationofpolyethyleneglycoldiacrylatepolycaprolactonescaffoldsfortissueengineeringapplications AT harshajamadagni evaluationofpolyethyleneglycoldiacrylatepolycaprolactonescaffoldsfortissueengineeringapplications AT morshedkhandaker evaluationofpolyethyleneglycoldiacrylatepolycaprolactonescaffoldsfortissueengineeringapplications |