EGR2, IGF1 and IL6 Expression Are Elevated in the Intervertebral Disc of Patients Suffering from Diffuse Idiopathic Skeletal Hyperostosis (DISH) Compared to Degenerative or Trauma Discs
Diffuse idiopathic skeletal hyperostosis (DISH) is characterised by ectopic ossification along the anterior spine and the outer intervertebral discs (IVD). However, the centre of the IVD, i.e., the nucleus pulposus, always remains unaffected, which could be due to the inhibition of the bone morphoge...
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MDPI AG
2021-04-01
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| author | Benjamin Gantenbein Rahel D. May Paola Bermudez-Lekerika Katharina A. C. Oswald Lorin M. Benneker Christoph E. Albers |
| author_facet | Benjamin Gantenbein Rahel D. May Paola Bermudez-Lekerika Katharina A. C. Oswald Lorin M. Benneker Christoph E. Albers |
| author_sort | Benjamin Gantenbein |
| collection | DOAJ |
| description | Diffuse idiopathic skeletal hyperostosis (DISH) is characterised by ectopic ossification along the anterior spine and the outer intervertebral discs (IVD). However, the centre of the IVD, i.e., the nucleus pulposus, always remains unaffected, which could be due to the inhibition of the bone morphogenetic protein (BMP) pathway. In this study, we investigated the transcriptome for the BMP pathway of DISH-IVD cells versus disc cells of traumatic or degenerative discs. The disc cells originated from nucleus pulposus (NP), annulus fibrosus (AF) and from cartilaginous endplate (CEP) tissue. Here, ninety genes of the transforming growth factor β-BMP signalling pathway were screened by qPCR. Furthermore, the protein expression of genes of interest was further investigated by immune-staining and semi-quantitative microscopy. IVDs of three DISH patients were tested against three control patients (same disc level and similar age). Early Growth Response 2 (<i>EGR2</i>) and Interleukin 6 (<i>IL6</i>) were both significantly up-regulated in DISH-IVD cells compared to controls (12.8 ± 7.6-fold and 54.0 ± 46.5-fold, respectively, means ± SEM). Furthermore, Insulin-like Growth Factor 1 (IGF1) tended to be up-regulated in DISH-IVD donors, i.e., 174.13 ± 120.6-fold. IGF1 was already known as a serum marker for DISH and other rheumatoid diseases and is confirmed here to play a possible key role in DISH-IVD. |
| first_indexed | 2024-03-10T11:49:53Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-10T11:49:53Z |
| publishDate | 2021-04-01 |
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| series | Applied Sciences |
| spelling | doaj.art-1b4a12e6f7d649208276b6cc805e65752023-11-21T17:48:19ZengMDPI AGApplied Sciences2076-34172021-04-01119407210.3390/app11094072EGR2, IGF1 and IL6 Expression Are Elevated in the Intervertebral Disc of Patients Suffering from Diffuse Idiopathic Skeletal Hyperostosis (DISH) Compared to Degenerative or Trauma DiscsBenjamin Gantenbein0Rahel D. May1Paola Bermudez-Lekerika2Katharina A. C. Oswald3Lorin M. Benneker4Christoph E. Albers5Tissue Engineering for Orthopaedics and Mechanobiology, Department for BioMedical Research (DBMR), University of Bern, CH-3008 Bern, SwitzerlandTissue Engineering for Orthopaedics and Mechanobiology, Department for BioMedical Research (DBMR), University of Bern, CH-3008 Bern, SwitzerlandTissue Engineering for Orthopaedics and Mechanobiology, Department for BioMedical Research (DBMR), University of Bern, CH-3008 Bern, SwitzerlandDepartment of Orthopaedic Surgery and Traumatology, Inselspital, University of Bern, CH-3010 Bern, SwitzerlandDepartment of Orthopaedic Surgery and Traumatology, Inselspital, University of Bern, CH-3010 Bern, SwitzerlandDepartment of Orthopaedic Surgery and Traumatology, Inselspital, University of Bern, CH-3010 Bern, SwitzerlandDiffuse idiopathic skeletal hyperostosis (DISH) is characterised by ectopic ossification along the anterior spine and the outer intervertebral discs (IVD). However, the centre of the IVD, i.e., the nucleus pulposus, always remains unaffected, which could be due to the inhibition of the bone morphogenetic protein (BMP) pathway. In this study, we investigated the transcriptome for the BMP pathway of DISH-IVD cells versus disc cells of traumatic or degenerative discs. The disc cells originated from nucleus pulposus (NP), annulus fibrosus (AF) and from cartilaginous endplate (CEP) tissue. Here, ninety genes of the transforming growth factor β-BMP signalling pathway were screened by qPCR. Furthermore, the protein expression of genes of interest was further investigated by immune-staining and semi-quantitative microscopy. IVDs of three DISH patients were tested against three control patients (same disc level and similar age). Early Growth Response 2 (<i>EGR2</i>) and Interleukin 6 (<i>IL6</i>) were both significantly up-regulated in DISH-IVD cells compared to controls (12.8 ± 7.6-fold and 54.0 ± 46.5-fold, respectively, means ± SEM). Furthermore, Insulin-like Growth Factor 1 (IGF1) tended to be up-regulated in DISH-IVD donors, i.e., 174.13 ± 120.6-fold. IGF1 was already known as a serum marker for DISH and other rheumatoid diseases and is confirmed here to play a possible key role in DISH-IVD.https://www.mdpi.com/2076-3417/11/9/4072low back paindiffuse idiopathic skeletal hyperostosisRNA extractionqPCRTGFβ-pathwayimmune-histochemistry |
| spellingShingle | Benjamin Gantenbein Rahel D. May Paola Bermudez-Lekerika Katharina A. C. Oswald Lorin M. Benneker Christoph E. Albers EGR2, IGF1 and IL6 Expression Are Elevated in the Intervertebral Disc of Patients Suffering from Diffuse Idiopathic Skeletal Hyperostosis (DISH) Compared to Degenerative or Trauma Discs Applied Sciences low back pain diffuse idiopathic skeletal hyperostosis RNA extraction qPCR TGFβ-pathway immune-histochemistry |
| title | EGR2, IGF1 and IL6 Expression Are Elevated in the Intervertebral Disc of Patients Suffering from Diffuse Idiopathic Skeletal Hyperostosis (DISH) Compared to Degenerative or Trauma Discs |
| title_full | EGR2, IGF1 and IL6 Expression Are Elevated in the Intervertebral Disc of Patients Suffering from Diffuse Idiopathic Skeletal Hyperostosis (DISH) Compared to Degenerative or Trauma Discs |
| title_fullStr | EGR2, IGF1 and IL6 Expression Are Elevated in the Intervertebral Disc of Patients Suffering from Diffuse Idiopathic Skeletal Hyperostosis (DISH) Compared to Degenerative or Trauma Discs |
| title_full_unstemmed | EGR2, IGF1 and IL6 Expression Are Elevated in the Intervertebral Disc of Patients Suffering from Diffuse Idiopathic Skeletal Hyperostosis (DISH) Compared to Degenerative or Trauma Discs |
| title_short | EGR2, IGF1 and IL6 Expression Are Elevated in the Intervertebral Disc of Patients Suffering from Diffuse Idiopathic Skeletal Hyperostosis (DISH) Compared to Degenerative or Trauma Discs |
| title_sort | egr2 igf1 and il6 expression are elevated in the intervertebral disc of patients suffering from diffuse idiopathic skeletal hyperostosis dish compared to degenerative or trauma discs |
| topic | low back pain diffuse idiopathic skeletal hyperostosis RNA extraction qPCR TGFβ-pathway immune-histochemistry |
| url | https://www.mdpi.com/2076-3417/11/9/4072 |
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