A comparative study of preservation and storage of Haemophilus influenzae
The aim of this study was to compare the efficacy of conservation by freezing the strains of Haemophilus influenzae at -20ºC and -70ºC. Skim milk supplemented with glucose, yeast extract and glycerol allowed highest viability of H. influenzae both at -20ºC and -70ºC from the media analyzed. Tryptica...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2001-05-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000400022 |
| _version_ | 1797757093225168896 |
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| author | Olga C Aulet de Saab Marta C de Castillo Aida P de Ruiz Holgado Olga M de Nader |
| author_facet | Olga C Aulet de Saab Marta C de Castillo Aida P de Ruiz Holgado Olga M de Nader |
| author_sort | Olga C Aulet de Saab |
| collection | DOAJ |
| description | The aim of this study was to compare the efficacy of conservation by freezing the strains of Haemophilus influenzae at -20ºC and -70ºC. Skim milk supplemented with glucose, yeast extract and glycerol allowed highest viability of H. influenzae both at -20ºC and -70ºC from the media analyzed. Trypticase soy broth and brain heart infusion broth supplemented with glycerol, allowed excellent recovery. Use of cotton swaps as supporting material, with or without addition of cryoprotective agents, did not modify H. influenzae viability after six months of storage. Concentration of the initial inoculum positively affected viability when stored at -20ºC. Initial concentration did not influence survival after storage at -70ºC. Thawing at room temperature should not exceed 3 h as to get highest survival percentage. |
| first_indexed | 2024-03-12T18:10:59Z |
| format | Article |
| id | doaj.art-1b4b5509e1cf45d186e68b7fcd851575 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:10:59Z |
| publishDate | 2001-05-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-1b4b5509e1cf45d186e68b7fcd8515752023-08-02T09:15:29ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602001-05-0196458358610.1590/S0074-02762001000400022A comparative study of preservation and storage of Haemophilus influenzaeOlga C Aulet de SaabMarta C de CastilloAida P de Ruiz HolgadoOlga M de NaderThe aim of this study was to compare the efficacy of conservation by freezing the strains of Haemophilus influenzae at -20ºC and -70ºC. Skim milk supplemented with glucose, yeast extract and glycerol allowed highest viability of H. influenzae both at -20ºC and -70ºC from the media analyzed. Trypticase soy broth and brain heart infusion broth supplemented with glycerol, allowed excellent recovery. Use of cotton swaps as supporting material, with or without addition of cryoprotective agents, did not modify H. influenzae viability after six months of storage. Concentration of the initial inoculum positively affected viability when stored at -20ºC. Initial concentration did not influence survival after storage at -70ºC. Thawing at room temperature should not exceed 3 h as to get highest survival percentage.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000400022Haemophilus influenzaestorageculture media |
| spellingShingle | Olga C Aulet de Saab Marta C de Castillo Aida P de Ruiz Holgado Olga M de Nader A comparative study of preservation and storage of Haemophilus influenzae Memorias do Instituto Oswaldo Cruz Haemophilus influenzae storage culture media |
| title | A comparative study of preservation and storage of Haemophilus influenzae |
| title_full | A comparative study of preservation and storage of Haemophilus influenzae |
| title_fullStr | A comparative study of preservation and storage of Haemophilus influenzae |
| title_full_unstemmed | A comparative study of preservation and storage of Haemophilus influenzae |
| title_short | A comparative study of preservation and storage of Haemophilus influenzae |
| title_sort | comparative study of preservation and storage of haemophilus influenzae |
| topic | Haemophilus influenzae storage culture media |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000400022 |
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